Abstract: To select suitable cultivation medium for‘Sweet Charlie’strawberry soilless culture under south sub-tropical climate, we studied the effect of six cultivation substrates on the growth and fruit quality of the strawberry. The results showed no significant difference of A (turf: vermiculite: pearlite: sand=7:1:1:1), B (turf: coco coir: vermiculite: pearlite: sand=5:2:1:1:1), C (turf: coco coir: vermiculite: pearlite: sand=(3.5:3.5:1:1:1) and CK on fruit quality and output, and they all performed well. The plant height, petiole length, petiole roughness and leaf area of A, B and C treatment were higher than those of CK. So, A, B and C could be used as the substrates of soilless culture to reduce the production cost.