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Chinese Agricultural Science Bulletin ›› 2019, Vol. 35 ›› Issue (1): 45-49.doi: 10.11924/j.issn.1000-6850.casb18070067

Special Issue: 园艺

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Wild Almond (Prunus tenella Batsch.) in Xinjiang: Screening of TP-M13-SSR Primers in Capillary Fluorescent Electrophoresis

  

  • Received:2018-07-14 Revised:2018-09-28 Accepted:2018-10-12 Online:2019-01-02 Published:2019-01-02

Abstract: The paper aims to observe the non-special peak appeared in capillary fluorescent electrophoresis and discuss its shape and the occurrence reason. We selected 2 samples of leaves from 5 populations of wild almond respectively to extract the genome DNA, and analyzed the amplification production by using the capillary fluorescent electrophoresis. The results showed that: there were 4 kinds of fluorescent primers in the capillary fluorescent electrophoresis; the peak of fluorescent primer FAM was within a normal range, with the special peak being obvious and the non-special peak did not occur frequently; by contrast, the non-special peak of fluorescent primer ROX had the most varieties, and the special peak was difficult to discern; in 6 exceptional cases, N+1 peak occurred the most and different peaks occurred the least. The fluorescent primer FAM is more suitable for wild almond to amplify.