Current issue

• 2019 Vol.35 No.1 Published:02 January 2019

  Characteristics of New Wheat Variety‘Yannong 173’with High Yield, Multi-resistance and Wide Adaptability 2019, 35 (1):  1-5.  DOI: 10.11924/j.issn.1000-6850.casb17110104 Abstract + ( 2591 )   PDF ( 1072KB )   ( 620 ) According to the regional and production test in Shandong Province during 2012- 2015, the differences of yield, yield components, quality and other aspects between ‘Yannong173’ and the control ‘Jimai 22’are analyzed. Methods of high stability coefficient (HSC) and coefficient of variation (CV) are used to determine the high and stable yield of‘Yannong173’. The results showed that the average yield of the variety was 8894.6 kg/hm2 in the tests, 5.68% more than that of the control, the difference was significant. The mean values of its high stability coefficient (HSC) and coefficient of variation (CV) of was 11.08 and 7.33, respectively, lower than the mean value (16.22 and 7.74) of the control, showing high and stable yield duality. Its grain was cutinization, had good commodity and good medium gluten quality.‘Yannong 173’has the advantages of high and stable yield, strong resistance and wide adaptability, and was a new wheat variety with wide application prospects.

  Winter Wheat Varieties Under Low Temperature: Glycine Betaine and Its Related Metabolic Pathways 2019, 35 (1):  6-10.  DOI: 10.11924/j.issn.1000-6850.casb17090080 Abstract + ( 1336 )   PDF ( 1058KB )   ( 298 ) The paper aims to study glycine betaine (GB) and the protection effect of betaine aldehyde dehydrogenase (BADH), which is the key synthetase, on wheat. The author planted two winter wheat varieties with different cold resistance,‘Dongnongdongmai1’(DM1) and‘Jimai22’(J22) under natural condition, and analyzed the relative electrical conductivity, malondialdehyde (MDA) content, BADH activity, the expression of BADH, and the content of GB at the seedling stage. The results showed that: DM1 presented a great increase in BADH activity and GB content, but a decrease in MDA content under freezing condition compared with J22, indicating that the expression of BADH promoted the accumulation of glycine betaine and increased the stability of cell membrane. The results of this study not only confirm the role of glycine betaine in cold resistance of DM1, but also provide a new research direction for improving the cold resistance of winter wheat in the future.

  Breeding of Naked Barley Variety‘Fumimai No. 2’ 2019, 35 (1):  12-16.  DOI: 10.11924/j.issn.1000-6850.casb17090046 Abstract + ( 1276 )   PDF ( 1025KB )   ( 336 ) The objective of this study is to breed a new naked barley variety that suitable for the ecological conditions in Fujian Province. The male parent was obtained from‘Zhu No.96005’induced by colchicine at tillering stage,‘No.04M85’introduced from Centro Internacional de Mejoramientode Maizy Trigo (CIMMYT) was used as the female parent, then the new naked barley variety‘Fumimai No.2’was obtained by cross breeding.‘Fumimai No.2’has high yield, excellent quality, big ears, big grain, stronger resistance to powdery mildew, and high economic benefit and so on, and it is suitable for planting in southeast Fujian Province. It is approved by Crop Variety Approval Committee of Fujian Province in 2016. The results indicate that it is feasible to breed new barley varieties induced by colchicine.

  Water Stress at Jointing and Tasseling Stage: Effect on Growth and Yield of Spring Maize 2019, 35 (1):  17-22.  DOI: 10.11924/j.issn.1000-6850.casb17100010 Abstract + ( 1347 )   PDF ( 1017KB )   ( 412 ) We designed 3 water treatments including CK (normal irrigation treatment), T1 (water stress since jointing stage) and T2 (water stress since tasseling stage) to discuss the effect of water stress on maize growth and yield. The results showed that: plant height and LAI of maize were sensitive to water stress under T1 treatment and the effects under T2 treatment were not significant; the contribution rate of the accumulated dry matter of vegetative organ to grain decreased under water stress especially the accumulated dry matter of leaves and stems decreased significantly; the accumulated dry matter of leaf sheaths was only sensitive to T1 treatment; water stress had great influence on the ear characteristics of maize and finally caused the yield under T1 and T2 treatment decreased by 62.8% and 37.8% compared with CK. Under water stress treatment, the influence factors on yield have an order as grain number> ear length> 100- grain weight> dry matter transport rate> ear diameter> LAI> biomass.

  Rapid and Large Scale Cultivation of Vigorous Seedlings of Small-grain Crops: Technique System Construction and Application 2019, 35 (1):  23-33.  DOI: 10.11924/j.issn.1000-6850.casb18080067 Abstract + ( 1239 )   PDF ( 1265KB )   ( 239 ) The paper aims at the difficulty to obtain a large number of vigorous seedlings of small-grain crops, such as foxtail millet, for genetic material extraction and purity identification by using traditional methods of breeding seedling. According to the rule of crop water requirement, we took loam as the matrix and created a breeding environment with higher moisture content in the low part and lower moisture content in the upper part to make seedlings grow vigorously and healthily. The results showed that: (1) a new technique system was set up for breeding seedlings, including“the choice of matrix, preparation of matrix with higher water content in the lower side, formation of seedling breeding matrix with higher water content in the lower side and lower water content in the upper side, controllable sowing and simple systematic management”; (2) 7 better and 1 optimum matrix ratios, and water content ranges of each part of matrix were screened out; (3) a large quantity of vigorous seedlings were cultivated by controllable sowing and simple systematic management at seedling stage. These methods are used in seedling breeding practice, and have obtained good results, which can provide technical support for the research of small-grain crops.

  Effects of Mixed Saline Stress on Photosynthetic Characteristics and Antioxidant Enzymes Activity of Cassava Seedlings 2019, 35 (1):  34-39.  DOI: 10.11924/j.issn.1000-6850.casb17090097 Abstract + ( 615 )   PDF ( 1108KB )   ( 223 ) The aim is to study the effects of mixed saline stress on photosynthetic characteristics and antioxidant enzymes of cassava seedling. We chose‘SC8’and‘GR911’as representative test materials and used potted method. The seedlings were treated with mixed saline treatment at 0 (CK), 0.05, 0.1, 0.15 and 0.2 mol/L. With different concentrations of mixed saline, chlorophyll contents, photosynthetic parameters and antioxidant enzymes activity of the cassava seedlings were determined and analyzed. The results showed that the chlorophyll contents in the leaves of‘SC8’and‘GR911’seedling went up first and then dropped under mixed saline stress, reached the highest when NaCl-Na2SO4 concentration was 0.15 mol/L, which was 2.52 and 2.35 mg/g, respectively. The photosynthetic parameters Pn, Tr, Gs, Ci, WUE and Ls increased first and then decreased, and the photosynthetic rate was‘SC8’＞’GR911’under low mixed saline stress, but the difference between‘SC8’and‘GR911’was not significant under high mixed saline stress. The enzyme activities of SOD, POD and CAT in‘GR911’were higher than those in‘SC8’under the same concentration of mixed saline stress. Comprehensive consideration of the effects of NaCl- Na2SO4 stress on photosynthetic indexes and antioxidant enzyme activities of‘SC8’and‘GR911’, NaCl-Na2SO4 tolerance of‘GR911’is higher than that of‘SC8’.

  Nutrient Change Rules of Nitrogen, Phosphorus and Potassium in Cassava Stalk Cultivation Substrate 2019, 35 (1):  40-44.  DOI: 10.11924/j.issn.1000-6850.casb17090113 Abstract + ( 504 )   PDF ( 981KB )   ( 230 ) The aim of this study is to clarify the nutrient change rules of nitrogen, phosphorus and potassium in cassava stalk cultivation substrate, so as to provide a scientific basis for the popularization and application of cassava stalk cultivation substrate and nutrient control in organic substrate cultivation. Under the condition of no adding exogenous nutrient, the change rules of nitrogen, phosphorus, potassium, pH value and EC value of cassava stalk substrate at different growth stages of tomato were compared under two conditions of planting tomato and no planting tomato by pot method. During the whole growth period of tomato, the cassava stalk substrate pH value was 7.28-7.86, and the EC value was 3.28-1432 μs/cm, both pH value and EC value conformed to the requirements of cultivation substrate. The changes of total nitrogen and alkaline hydrolysis nitrogen were“down- up- down”. The contents of total phosphorus and available phosphorus showed an increased trend. Before tomato seedling stage, the contents of total potassium and available potassium decreased rapidly due to leaching, but they kept a stable level after tomato seedling stage. The nutrient change rules of nitrogen, phosphorus and potassium in“cassava stalk cultivation substrate + tomato”were similar to the nutrient change rules of“cassava stalk cultivation substrate”. The nutrient contents of nitrogen, phosphorus and potassium with the two treatments were almost at the same level. The seasonal tomato adsorbed few nutrient of nitrogen, phosphorus and potassium from cassava stalk cultivation substrate. Therefore, when we cultivate plants in cassava stalk cultivation substrate, we must supply fertilizer in time to ensure the healthy growth of plants.

  Wild Almond (Prunus tenella Batsch.) in Xinjiang: Screening of TP-M13-SSR Primers in Capillary Fluorescent Electrophoresis 2019, 35 (1):  45-49.  DOI: 10.11924/j.issn.1000-6850.casb18070067 Abstract + ( 587 )   PDF ( 1555KB )   ( 228 ) The paper aims to observe the non-special peak appeared in capillary fluorescent electrophoresis and discuss its shape and the occurrence reason. We selected 2 samples of leaves from 5 populations of wild almond respectively to extract the genome DNA, and analyzed the amplification production by using the capillary fluorescent electrophoresis. The results showed that: there were 4 kinds of fluorescent primers in the capillary fluorescent electrophoresis; the peak of fluorescent primer FAM was within a normal range, with the special peak being obvious and the non-special peak did not occur frequently; by contrast, the non-special peak of fluorescent primer ROX had the most varieties, and the special peak was difficult to discern; in 6 exceptional cases, N+1 peak occurred the most and different peaks occurred the least. The fluorescent primer FAM is more suitable for wild almond to amplify.

  Development of Plant Leaf Margin: Advances in Research 2019, 35 (1):  50-56.  DOI: 10.11924/j.issn.1000-6850.casb18070050 Abstract + ( 784 )   PDF ( 949KB )   ( 283 ) Leaf margin serration is one of morphological characteristics, which is controlled by various leaf margin factors. We reviewed domestic and foreign research results, introduced in detail the effect of plant hormone, CUC2(CUP SHAPED COTYLEDONE2) and MicroRNA on leaf margin serration development, summarized the expression and function of leaf margin factors, discussed the molecular mechanism and significance of leaf margin. Analysis showed that auxin and CUC2 had significant function in the leaf margin regulatory network and most leaf margin factors rely on auxin and CUC2. In the end, we proposed future research direction of leaf margin serration development and forecasted its application fields.

  Chlorogenic Acid and Rutin from Inferior and Waste Tobacco: Extraction Process 2019, 35 (1):  57-62.  DOI: 10.11924/j.issn.1000-6850.casb17120090 Abstract + ( 545 )   PDF ( 1233KB )   ( 230 ) The paper aims to find an appropriate extraction process of chlorogenic acid and rutin from inferior and waste tobacco to improve its economic added value. The authors adopted the single factor experiment and response surface design, detected extraction yield by the high performance liquid chromatography (HPLC), optimized the parameters of the extraction process by ethanol concentration, liquid-solid ratio, extraction time and extraction temperature. The results showed that: the optimized condition for extraction of chlorogenic acid and rutin from inferior and waste tobacco was ethanol concentration of 67%, liquid-solid ratio of 1:16 (g:mL), extraction temperature of 61℃ and the extraction time of 150 min. Under this modified condition, the sum of the theoretical extraction yield of chlorogenic acid and rutin was 43.59 mg/g, and the relative error was 0.36%. Analysis of the Design-Expert showed that: ethanol concentration>extraction time>liquid-solid ratio>extraction temperature. The technological condition of extracting chlorogenic acid and rutin from inferior and waste tobacco obtained by the study could provide a new way for better utilization of waste tobacco.

  Nutrient Concentrations Affect the Biomass and Nitrate Content of Brassica campestris L. 2019, 35 (1):  63-68.  DOI: 10.11924/j.issn.1000-6850.casb18070052 Abstract + ( 646 )   PDF ( 1050KB )   ( 330 ) To find the best water-manure supply method of reducing nitrate content without affecting biomass of Brassica campestris L., and provide reference for the economic and environmental fertilizing method with low nitrate residue, the effect of different EC value nutrient solutions on the biomass, soakage and nitrate content of B. campestris L. was studied. The results showed that in the 10 days of field setting, the concentration of nutrient solution rarely influenced the physical indexes. After 10 days, the physical indexes increased with the increase of nutrient solution concentration below 2.4 dS/m, and it had no significant effect on fresh weight when the concentration was higher than or equal to 2.4 dS/m. The nitrate content obviously increased in harvest time when EC value was more than or equal to 2.4 dS/m. The water absorbing ability of B. campestris L. was positively correlated with the concentration of nutrient solution, and water use efficiency reached the highest under nutrient solution concentration of 2.4 dS/m. The best culture condition is: 1.2 dS/m in the first 10 days of field setting and then 2.4 dS/m instead.

  SRAP Analysis of 26 Volvariella volvacea Strains Cultivated in Shandong 2019, 35 (1):  69-74.  DOI: 10.11924/j.issn.1000-6850.casb18010168 Abstract + ( 546 )   PDF ( 1022KB )   ( 227 ) To fully utilize and protect the germplasm resources of Volvariella volvacea, the authors analyzed the genetic diversity of 26 V. volvacea strains from different provinces and regions by using SRAP-PCR, and extracted DNA adopting the improved SDS method. Among 153 pairs of primers composed of 9 positive primers and 17 reverse primers, 11 pairs of polymorphic primers were screened and 148 polymorphic loci were amplified, of which 142 were polymorphic loci, the fragment size was between 0.11- 2.51 kb, the ratio of polymorphic loci was between 75.0%-100%, and the polymorphism ratio was 95.5%. Cluster analysis of 26 V. volvacea varieties was carried out by NTSYS-PC2.1e software, the results showed that: when the similarity coefficient was 0.76, 26 strains of V. volvacea could be divided into 3 groups; among them, 19 strains of Tianda V971, Gaoyou grass V112 and single V. volvacea were clustered into one class, and 6 strains of the second group included V23, V11, 931, V23, Gaoyou V11, and V23, and silver fillet V. volvacea was clustered into a class. It indicates that after being introduced to each other, V. volvacea is re-preserved and named. At the same time, V. volvacea has regional gene differentiation to some extent. Exploring the molecular gene pool of V. volvacea could provide basic data for the establishment of V. volvacea germplasm resource bank, and the breeding and genetic improvement of V. volvacea in Shandong.

  Molecular Characteristics and Temporal and Spatial Expression Patterns of Soluble Starch Synthase III-1 Gene (MaSSIII-1) in Banana (Musa acuminata) Fruit 2019, 35 (1):  75-79.  DOI: 10.11924/j.issn.1000-6850.casb18070072 Abstract + ( 578 )   PDF ( 1866KB )   ( 259 ) To clarify the molecular characteristics and temporal and spatial expression patterns of soluble starch synthase III-1 gene (MaSSIII-1), using the‘Brazilian’banana pulps as the test material, the MaSSIII-1 gene was cloned by PCR method. Quantitative real-time PCR (qPCR) and Western blot technology were used to analyze MaSSIII- 1 gene and its protein expression pattern. The results showed that the cDNA full-length of MaSSIII-1 gene (accession No. KU757067) was 2397 bp encoding 798 amino acids with four typical domains. The expression level of MaSSIII-1 was low in roots, bulbs, flowers, and bracts, but it exhibited high expression in leaves, peels, and pulps. Moreover, MaSSIII- 1 expression increased gradually with banana fruit development, and then reached the highest expression in 50-60 d after emergence from the pseudostem. The expression level of MaSSIII-1 reached the maximum value in 5 d after harvest and then decreased gradually during postharvest ripening in banana fruit. The expression pattern of MaSSIII-1 protein was consistent with the expression trend at mRNA level during banana fruit development and ripening. These results suggested that the expression of MaSSIII- 1 gene may involve not only amylopectin biosynthesis but also early fruit ripening or amylopectin degradation in banana.

  Interspecific relationships and niche of dominant herbaceous plants under different plantations on the reclaimed coal gob pile in the Yangquan mining area of Shanxi Province, China 2019, 35 (1):  80-87.  DOI: 10.11924/j.issn.1000-6850.casb18060080 Abstract + ( 721 )   PDF ( 1112KB )   ( 298 ) To reveal the interspecific relationship and niche characteristics of the dominant species of herb communities under different vegetations on the reclaimed coal gob pile, based on the plant plot sampling, we analyzed the interspecific relationships of 12 dominant species under different vegetations revegetated in 2003 on the reclaimed coal gob pile in No. 5 mine in the Yangquan mining area of Shanxi by the methods of χ2 test, Pearson correlation coefficient, Spearman rank correlation coefficient, niche breadth and overlap. The results showed that: (1) the positive and negative correlation ratio of the 12 dominant species of herb community under different vegetations was less than 1, and there were negative correlation among the whole 12 dominant species, and there were few species-pairs with very significant and significant correlations, the relationship between the species was loose and the correlation was poor; (2) the 12 dominant species under different vegetations were classified into 3 ecological species groups, and soil moisture was the key environmental factor resulting in the differentiation of these species groups; (3) the interspecific relationship was consistent with the value of niche overlap, while there were no direct linear relationship between their niche breadth and overlap; (4) the naturally colonized plants such as Bothriochloa ischaemum, Artemisia capillaris and Heteropappus altaicus had strong ability of ecological adaptability and survival competitiveness to bear the poor habitat of coal gob pile. The stability of the herb communities on the reclaimed coal gob pile in the Yangquan mining area of Shanxi is poor, and the herb communities are still in the early or middle stage of succession. The naturally colonized herbaceous plant B. ischaemum, as a best pioneer species, could be recommended for revegetation of local coal gob piles, but it has negatively interspecific relationship with the planted Festuca elata, and thus the two could not be recommended as species-pairs for mixed planting revegetation of local coal gob piles.

  The Special and Temporal Evaluation and Correlation Analysis of Ecological Civilization of Henan Province 李小弯 and 李青松 2019, 35 (1):  88-94.  DOI: 10.11924/j.issn.1000-6850.casb18050059 Abstract + ( 588 )   PDF ( 1175KB )   ( 636 ) To understand the level of regional ecological civilization and its evolutionary characteristics and influencing factors, we constructed an evaluation system of ecological civilization from four aspects of ecological status, ecological protection, ecological economy and ecological culture. Then, we evaluated the ecological civilization level of Henan Province from 2005 to 2015 by grey correlation model. The results showed that: (1) the average value of ecological civilization index was 0.460, and the average annual growth rate was 1.92% ; the overall level was gradually improved, but the regional growth was not balanced; the number of cities with high-level ecological civilization increased from 2005 to 2015, and the higher the level, the faster the growth rate; (2) there were significant regional differences of ecological civilization in Henan, the spatial characteristics were of higher levels in southwestern Henan and of lower levels in northern Henan; (3) the ecological civilization index of each subsystem was following the order of ecological status> ecological culture> ecological protection> ecological economy; the correlation between ecological civilization index (C1), ecological status (C2) and ecological culture (C5) were the highest, and the correlation coefficient was 0.9454 and 0.9210, respectively, with significant correlation (P<0.05); (4) the factors affecting the level of ecological civilization in Henan were per capita regional production (X25), per capita arable land (X6), per capita park green area (X8), the share of environmental pollution treatment in GDP (X17), and the share of the tertiary industry in GDP (X 18).

  The Climatic Suitability Regionalization of Two-line Hybrid Rice Seed Production in ChengduSuitability Regionalization of Two-line Hybrid Rice Seed Production in Chengdu 王芬,亢强, and 2019, 35 (1):  95-103.  DOI: 10.11924/j.issn.1000-6850.casb17090115 Abstract + ( 497 )   PDF ( 1814KB )   ( 349 ) The paper aims to solve the problem of two-line hybrid rice seed in situ production in Chengdu. Using meteorological diagnosis method based on risk analysis, the authors analyzed the security and suitability in the 3 key periods of two-line hybrid rice seed production (thermo-sensitive period of the two-line male sterile lines, heading and flowering period of the parents, seeds maturation and harvesting period) of 13 districts, cities or counties in Chengdu, and got the suitable areas and time ranges for the two-line hybrid rice seed production. The results showed that: the comprehensive risk index of heading and flowering in most of the time ranges analyzed was less than 4.0, the time intervals were 44-95 days, which provided sufficient time intervals for the selection of safe time ranges during heading and flowering period for two-line hybrid rice seed production. So, the suitability of the seed production base was mainly determined by the thermo-sensitive period. At the temperature threshold of 22.5℃, the most suitable seed production areas for two-line hybrid rice were Jintang, Longquanyi, Qionglai, Pujiang, Shuangliu and Xinjin; at the temperature threshold of 23℃, the suitable areas were Xinjin, Jintang, Xindu, Dayi and Shuangliu; at the temperature threshold of 23.5℃ and 24.0℃, the areas suitable were very rare in Chengdu. If seed production of two-line hybrid rice is conducted in Chengdu, to lower the seed purity risk, the producer should choose those two-line male sterile lines with the critical sterility inducing temperature (CSIT) lower than 23℃ and relatively short thermo-sensitive period.

  Extraction of Rice Planting Area in Dabie Mountain by Remote Sensing Image: Taking Landsat 8 as an Example 2019, 35 (1):  104-111.  DOI: 10.11924/j.issn.1000-6850.casb17090096 Abstract + ( 752 )   PDF ( 1904KB )   ( 362 ) We selected Dabie Mountain in Anqing of Anhui Province as the study area, including Taihu, Yuexi, Susong and Qianshan, and analyzed the rice planting area distribution, and conducted the dynamic realtime monitoring of rice planting area by 6 Landsat 8 satellite remote sensing images from 2016 and 2017. We extracted the planting area of late rice in the study area by remote sensing interpretation method, and verified the accuracy by random point test and Kappa coefficient. The results showed that: the extraction accuracy of rice planting area in 2017 and 2016 was 93.44%, 93.78%, respectively, and the Kappa coefficient was 0.86, 0.83, respectively, proving that the extraction result was accurate; we compared the planting area of late rice in 2017 and 2016, found that the change rate of rice planting area in Taihu, Qianshan and Yuexi was less than 5%, which was a normal one; since some farmland in Susong was flooded in 2016, so its rice planting area in 2017 increased by 13.01% . Compared with traditional statistical methods of crop acreage, the method of remote sensing can save manpower, material and financial resources, and can achieve real-time and dynamic monitoring of crops accurately and quickly.

  First, Last Frost Dates and Frost-free Period During 1961-2015 in Shouguang: Variation Characteristics and Its Effect on Agriculture 秦瑜蓬 2019, 35 (1):  112-117.  DOI: 10.11924/j.issn.1000-6850.casb18020031 Abstract + ( 786 )   PDF ( 1193KB )   ( 312 ) The paper aims to provide reference for disaster prevention and mitigation of agricultural production in Shouguang, based on the climatic data (first, last frost date and frost-free period) from 1961 to 2015 in Shouguang by using modern climate diagnostic analysis method, we analyzed the variation characteristics of first, last frost date and frost-free period, and evaluated the influence of first, last frost date on yield of winterwheat and cotton. The results showed that: (1) first frost date showed a delayed trend, but last frost date was ahead of time, leading to an increase in frost-free period; (2) first frost date was postponed and the climate trend rate was 2.23 d/10 a, a mutation existed in 1991; (3) last frost date was advanced with the tendency rate of -1.35 d/10 a; (4) frost-free period increased with the tendency rate of 3.58 d/10 a and had a mution in 1994; (5) first frost day and frost-free period were positively correlated with the yield of wheat and cotton, and last frost date was negatively correlated with the yield of wheat and cotton, and the delay of first frost day made more contribution to the yield of wheat and cotton than that of the advacing of last frost day.

  Sweetpotato Virus Diseases (SPVD): Research Progress 2019, 35 (1):  118-126.  DOI: 10.11924/j.issn.1000-6850.casb18060093 Abstract + ( 551 )   PDF ( 1582KB )   ( 372 ) Sweetpotato virus disease (SPVD), which is caused by the dual infection and synergistic interaction of Sweetpotato feathery mottle virus (SPFMV) and Sweetpotato chlorotic stunt virus (SPCSV), is a devastating viral disease of sweetpotato. Since reported in China in 2012, it has been expended rapidly, and in 2015 it caused huge economic losses to sweetpotato production in Zhanjiang of Guangdong. In order to strengthen the prevention and control of sweetpotato virus disease and reduce losses, the distribution and harm of SPVD are summarized, and the gene structure and function, transmission and detecting means of SPFMV and SPCSV are reviewed respectively. It is suggested that in serious disease areas, detection of virus strains should be a key point, and molecular mechanism study about synergism between SPFMV and SPCSV should be further explored, thus to achieve the timely detection, early prevention and non-toxic cultivation of sweetpotato.

  Potato-staple Food: Nutrition Cognition and Consumption Behavior of Beijing Residents 2019, 35 (1):  127-133.  DOI: 10.11924/j.issn.1000-6850.casb17090152 Abstract + ( 614 )   PDF ( 1019KB )   ( 301 ) The paper aims to ascertain the influence factors of consumers’nutrition cognition and purchase choice of potato staple food products. Based on the data of the consumption guide experience of potato staple food collected from Beijing communities in 2016, the authors deeply discussed the influence of consumer’s individual characteristics, dietary habits, socio- economic status and cognition levels on the acceptance to potato staple food and the willingness to buy and consume. The results showed that: consumers’age, weight, dietary habits, nutritional knowledge levels, food safety risk perception, and price and so on had significant effects on their acceptance of potato staple food, but the influence directions were different, indicating that the degree of awareness and understanding of potato staple food was still limited and there was misunderstood. Therefore, the study proposes to actively carry out various kinds of food nutrition popularization activities, in order to promote the public nutrition cognition level of potato staple food, and initiate more active acceptance and consumption of potato staple food.

  The Determination of Xylooligosaccharide in Brewer''s Grains Hydrolysate Based on HPLC-ELSD Method 2019, 35 (1):  134-143.  DOI: 10.11924/j.issn.1000-6850.casb17100070 Abstract + ( 500 )   PDF ( 1817KB )   ( 216 ) The objective is to establish the HPLC-ELSD detection method of xylooligosaccharide in brewer''s enzymatic hydrolysis. Brewer''s grains hydrolysate was extracted with pure water, chromatographic column was COSMOSIL Sugar-Danalytical column (250 mm×4.6mm, 5 μm), the mobile phase was acetonitrile-water (69: 31, V/V), the flow rate was 0.9 mL/min, the column temperature was 40℃, the injection volume was 3 μL, the evaporator temperature was 78℃, flow rate of carrier gas was 1.6 L/min and carrier gas pressure was 0.5 MPa, and the content of xylooligosaccharide was determined by using the peak area external standard method. The results showed that Xylobiose-Xylopentaose had good linear relation in the range of 0.024-0.245 mg/mL, the detection limits were 0.0245, 0.0245, 0.02425, 0.024 mg/mL, and the limits of quantification were 0.0907, 0.0907, 0.08981, 0.8889 mg/mL. The relative standard deviations (RSD) were 0.203%, 0.224%, 0.233% and 0.252% and the spiked recoveries rates were 97.31%-99.83%. The results indicated that this method had a high sensitivity and good repeatability and could be applied to detect xylooligosaccharide in brewer''s grains.

  The Inhibitory Effect of Extracts from Platycodon grandiflorum and Hedyotis diffusa Willd on Human Lung Cancer Cells A549 2019, 35 (1):  144-148.  DOI: 10.11924/j.issn.1000-6850.casb18070049 Abstract + ( 501 )   PDF ( 1093KB )   ( 272 ) To screen the effective antitumor components of Platycodon grandiflorum (Jacq.)A.DC. and Hedyotis diffusa Willd, total flavonoids and total saponins of Platycodon grandiflorum, total flavonoids and total anthraquinones of Hedyotis diffusa Willd were prepared, the content of total flavonoids was determined by sodium nitrite - aluminum nitrate colorimetric method, the content of total saponins was determined by vanillin assay, the content of total anthraquinones was determined by ultraviolet spectromter method, the inhibitory effects of four extracts on human lung cancer cells A549 were determined by methyl thiazolyl tetrazolium (MTT) assay. The results indicated that, contents of total flavonoids from Platycodon grandiflorum and Hedyotis diffusa Willd were 5.51% and 1.19%, content of total saponins from Platycodon grandiflorum was 1.71%，content of total anthraquinones from Hedyotis diffusa Willd was 7.81%。The inhibition rate of 0.04 mg/mL total saponins from Platycodon grandiflorum on human lung cancer cells A549 was 87.59%，the inhibition rate of 0.5 mg/mL total flavonoids from Platycodon grandiflorum was 89.16%，the inhibition rate of 1.2 mg/mL total anthraquinones from Hedyotis diffusa Willd was 34.76%，the inhibition rate of 0.3 mg/mL total flavonoids from Hedyotis diffusa Willd was 24.33%。Total flavonoids and saponins from Platycodon grandiflorum have strong antitumor activity，which provides some powerful reference for the development of related antitumor products.

  Safe Utilization of Prometryne in Sea Cucumber Culture Pond: From Nutrient Diffusion Flux Across Biodeposition-water Interface Aspect 2019, 35 (1):  149-153.  DOI: 10.11924/j.issn.1000-6850.casb18060081 Abstract + ( 590 )   PDF ( 1003KB )   ( 292 ) Sea cucumber is cultured generally in temperate zone. Macroalgae may outbreak in sea cucumber culture ponds, which influence its survival and growth. Macroalgae are killed by prometryne generally. It is supposed that prometryne might affect the nutrients regeneration in biodeposition because of herbicide toxicity. To evaluate the hypothesis, the sea cucumber feces were exposed to prometryne with different concentrations for 7 days and the nutrients diffusion fluxes across the biodeposition-water interface were studied. The results showed that the nutrients diffusion fluxes across the biodeposition-water interface and the construction of nutrients released from the biodeposition were not influenced by prometryne of normal dosage used to control macroalgae, hence, the prometryne could not affect the regeneration/accumulation of nutrients in biodeposition. It is no necessary to worry about the utilization of prometryne in sea cucumber culture ponds. Results of the present work may shed light on evaluating effects of herbicide on nutrients cycle in seawaters.

  Influencing Factors and Forecasting Methods of Grain Yield in Henan Province 2019, 35 (1):  154-158.  DOI: 10.11924/j.issn.1000-6850.casb17070066 Abstract + ( 657 )   PDF ( 1011KB )   ( 378 ) Grain production is an important part of the national economy, and grain yield is significant for guaranteeing grain security in China. In order to improve the scientific and accurate prediction of grain yield, based on the analysis of the existing prediction methods, the grey theory and neural network were combined organically, the main and objective factor indexes were determined from many influencing factors on grain yield by grey correlation analysis. These indexes were applied in the prediction of grain yield with the artificial neural network. The results showed that the maximum error of the artificial neural network prediction was 1.21%, the average error was 0.63%, and the prediction accuracy was relatively high. This study provides a scientific and effective forecasting method for grain yield.

  The Competitiveness and Complementarity of Agricultural Products Trade Between China and Thailand 2019, 35 (1):  159-164.  DOI: 10.11924/j.issn.1000-6850.casb17090077 Abstract + ( 759 )   PDF ( 993KB )   ( 456 ) In order to study the competitiveness and complementarity of agricultural products trade between China and Thailand, the RCA index and TCI index were used to analyze its competitiveness and complementarity. The results showed that the trade volume of agricultural products between China and Thailand expanded from 2006 to 2015, and the trade volume of vegetables, fruits, aquatic products, cotton, grain and sugar were large. The RCA index of Chinese agricultural products was less than 1, and the RCA index of agricultural products in Thailand was greater than 1; TCI index on China''s agricultural exports to Thailand agricultural imports was less than 1, TCI index on Thailand agricultural exports to China''s agricultural imports was greater than 1 from 2008 to 2015.