Response Surface: Optimizing the Medium for Gliotoxin Produced by Trichoderma viride

doi:10.11924/j.issn.1000-6850.casb18080086

Abstract

Abstract: Response surface method was used to optimize the culture medium for gliotoxin production by Trichoderma viride. Evaluation of the effects of the 6 factors relating to the basic medium was carried out through Min Run Res IV design, which revealed the significant influence factors. Steepest ascent design(SAD) and central composite design(CCD) were applied to determine the midpoint and the optimal value respectively, and the optimized culture medium was obtained. Glucose, soymeal, KH2PO4 and ZnSO4 were the most significant influence factors screened by Min Run Res IV. Response surface analysis of the SAD and CCD indicated that the optimal culture medium was composed of 26.54 g/L glucose,10 g/L yeast powder,15.72 g/L soymeal, 0.452g/L KH2PO4, 0.0582g/L ZnSO4 and 0.1g/L FeSO4, which resulted in a 59.71% increase of the titer of gliotoxin compared with the original medium.

CLC Number:

Q815

韩立霞 and 刘海玉. Response Surface: Optimizing the Medium for Gliotoxin Produced by Trichoderma viride[J]. Chinese Agricultural Science Bulletin, 2019, 35(22): 42-46.

References

[1] 周万青,沈瀚,张之烽,等.烟曲霉胶霉毒素的研究进展[J].中国真菌学杂志,2011,6(2)：118-121.
[2]Auvin-Guette C,Rebuffot S,Prigent Y,Bodo B.1992.Triehogin A IV,an 11-residue 1ipopeptaibol from Trichoderma1ignorum.Journal of the American Chemieal Soeiety,114:2170-2174
[3]Avent A G,Hanson J R,Truneh A.1993.Metabolites of Gliocladium flavofuscum.Phytoehemistry 32:197-198.
[4]刘路宁,屠艳拉,张敬泽.绿木霉菌株 TY009防治纹枯病等水稻主要真菌病害的潜力[J].中国农业科学,2010,43(10)：2031-2038.
[5] Lee F N,Rush M C.Rice sheath blight:A major rice disease[J].Plant Disease,1983,67:829-832.
[6]焦亚楠,陈敏.胶霉毒素毒性机制研究进展[J].中国公共卫生,2013,29(11)：1656-1658.
[7]Hof H, Kupfahl C. Gliotoxin in Aspergillus fumigatus:an example that mycotoxins are potential virulence factors[J]. Mycotoxin Research,2009,25( 3):123-131.
[8] Kosalec I, Pepeljnjak S. Mycotoxigenicity of clinical and environmental Aspergillus fumigatus and A.flavus isolates[J]. Acta Pharmaceutica,2005,55(4):365-375.
[9] 朱廷恒,邢小平,孙顺娣．木霉T97菌株对几种植物病原真菌的拮抗作用机制和温室防治试验[J]. 植物保护学报,2004,31(2)：139-144．
[10]姚彦坡,王雅玲,吕国忠．木霉对草坪上2种重要土传病害生防效果的研究[J]．草业科学,2007,24(8)：96-99.
[11] JONES R W, HANCOCK J G. Mechanism of gliotoxin action and factors mediating gliotoxin sensitivity[J].J GenMicrobiol,1988,134:2067-2075.
[12] 孙勇．绿色木霉的拮抗机理、发酵条件、生物防治的研究[D]．西安：西北大学,2003：20－21．
[13] PAPVIZAS G C.Evaluation of new biotypes of Trichdermaharziaum for tolerance tobenomyl enhanced capabilities[J].Phytopathology,1982,72:126-132．
[14]李余生,朱镇,张亚东,等.水稻稻曲病抗性基因+多基因混合遗传模型分析[J].作物学报,2008,34(10)：1728-1733.
[15] Grsgorv K F, Allen O N, Riker A J, Peterson W H. Antibiotics asagents for the control of certain damping-off fungi. Botanical Society of America, 1952, 39: 405-415.
[16] Howell C R, Stipanovic R D, Lumsden R D. Antibiotic production by strains of Gliocladium virens and its relation to the biocontrol of cotton seedling diseases. Biocontrol Science and Technology, 1993, 3:435-441.
[17]田泱源,李瑞芳. 响应面法在生物过程优化中的应用[J]. 食品工程,2010(2)：8－11.
[18]Montgomery D C.Design and analysis of experiments[M].New York:John Wiley Sons,2000:46-51.
[19] 汪彬彬, 车振明. Plackett-Burman和Box-Benhnken Design实验设计法优化华根霉产糖化酶发酵培养基的研究[J]. 食品科技,2011(5)： 41－45.
[20] 李勇昊,姜永生,周长海,等.响应面法优化里氏木霉Rut C-30产纤维素酶液体培养基[J]. 中国酿造,2012(4)：29-32.