Welcome to Chinese Agricultural Science Bulletin,
Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (36): 84-92.doi: 10.11924/j.issn.1000-6850.casb20200100045
Special Issue: 生物技术
Previous Articles Next Articles
Yang Dandan1(
), Yang Chuanlun1, Zhang Xinqing1, Pan Dongmei1, Li Jiaming2, Chen Zhenfa1
Received:2020-01-14
Revised:2020-02-23
Online:2020-12-25
Published:2020-12-23
CLC Number:
Yang Dandan, Yang Chuanlun, Zhang Xinqing, Pan Dongmei, Li Jiaming, Chen Zhenfa. Response Surface Methodology Applied to Spores Production by Solid-state Fermentation of Trichoderma Viride[J]. Chinese Agricultural Science Bulletin, 2020, 36(36): 84-92.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb20200100045
| 编号 | A | B | D | E | G | H | 孢子数/（×109个/g） |
|---|---|---|---|---|---|---|---|
| 1 | -1 | 1 | -1 | 1 | 1 | -1 | 6.20 |
| 2 | -1 | -1 | -1 | -1 | -1 | -1 | 5.10 |
| 3 | 1 | 1 | -1 | -1 | 1 | -1 | 5.41 |
| 4 | 1 | -1 | -1 | 1 | 1 | 1 | 6.19 |
| 5 | 1 | -1 | 1 | -1 | 1 | 1 | 5.50 |
| 6 | -1 | 1 | 1 | 1 | 1 | 1 | 4.52 |
| 7 | -1 | -1 | 1 | -1 | 1 | -1 | 6.75 |
| 8 | 1 | -1 | 1 | 1 | -1 | -1 | 4.40 |
| 9 | 1 | 1 | -1 | -1 | -1 | 1 | 3.23 |
| 10 | -1 | 1 | 1 | -1 | -1 | 1 | 2.30 |
| 11 | 1 | 1 | 1 | 1 | -1 | -1 | 3.38 |
| 12 | -1 | -1 | -1 | 1 | -1 | 1 | 4.13 |
| 编号 | A | B | D | E | G | H | 孢子数/（×109个/g） |
|---|---|---|---|---|---|---|---|
| 1 | -1 | 1 | -1 | 1 | 1 | -1 | 6.20 |
| 2 | -1 | -1 | -1 | -1 | -1 | -1 | 5.10 |
| 3 | 1 | 1 | -1 | -1 | 1 | -1 | 5.41 |
| 4 | 1 | -1 | -1 | 1 | 1 | 1 | 6.19 |
| 5 | 1 | -1 | 1 | -1 | 1 | 1 | 5.50 |
| 6 | -1 | 1 | 1 | 1 | 1 | 1 | 4.52 |
| 7 | -1 | -1 | 1 | -1 | 1 | -1 | 6.75 |
| 8 | 1 | -1 | 1 | 1 | -1 | -1 | 4.40 |
| 9 | 1 | 1 | -1 | -1 | -1 | 1 | 3.23 |
| 10 | -1 | 1 | 1 | -1 | -1 | 1 | 2.30 |
| 11 | 1 | 1 | 1 | 1 | -1 | -1 | 3.38 |
| 12 | -1 | -1 | -1 | 1 | -1 | 1 | 4.13 |
| 试验号 | 碳酸钙/% | 硫酸铵/% | 氯化锰/% | 孢子数/（×109个/g） |
|---|---|---|---|---|
| 1 | 0.30 | 2.0 | 0.60 | 3.20 |
| 2 | 0.40 | 1.7 | 0.52 | 5.05 |
| 3 | 0.50 | 1.4 | 0.44 | 6.20 |
| 4 | 0.60 | 1.1 | 0.36 | 7.52 |
| 5 | 0.70 | 0.8 | 0.28 | 5.86 |
| 6 | 0.80 | 0.5 | 0.20 | 4.05 |
| 试验号 | 碳酸钙/% | 硫酸铵/% | 氯化锰/% | 孢子数/（×109个/g） |
|---|---|---|---|---|
| 1 | 0.30 | 2.0 | 0.60 | 3.20 |
| 2 | 0.40 | 1.7 | 0.52 | 5.05 |
| 3 | 0.50 | 1.4 | 0.44 | 6.20 |
| 4 | 0.60 | 1.1 | 0.36 | 7.52 |
| 5 | 0.70 | 0.8 | 0.28 | 5.86 |
| 6 | 0.80 | 0.5 | 0.20 | 4.05 |
| 试验号 | 碳酸钙(A) | 硫酸铵(B) | 氯化锰(C) | 孢子数/（×109个/g） |
|---|---|---|---|---|
| 1 | -1 | -1 | 1 | 7.05 |
| 2 | 0 | 0 | 0 | 8.40 |
| 3 | 1 | -1 | 1 | 6.40 |
| 4 | 0 | 0 | 0 | 8.40 |
| 5 | 0 | 0 | 1.681793 | 7.97 |
| 6 | -1 | -1 | -1 | 5.95 |
| 7 | -1 | 1 | -1 | 5.72 |
| 8 | -1 | 1 | 1 | 6.72 |
| 9 | 1.681793 | 0 | 0 | 5.60 |
| 10 | 1 | 1 | -1 | 4.50 |
| 11 | 1 | 1 | 1 | 6.06 |
| 12 | 0 | 0 | 0 | 8.40 |
| 13 | 0 | 0 | 0 | 8.40 |
| 14 | 0 | 1.681793 | 0 | 7.02 |
| 15 | 0 | 0 | -1.68179 | 6.98 |
| 16 | 0 | 0 | 0 | 8.40 |
| 17 | 0 | 0 | 0 | 8.40 |
| 18 | 0 | -1.68179 | 0 | 7.30 |
| 19 | 1 | -1 | -1 | 7.39 |
| 20 | -1.68179 | 0 | 0 | 6.27 |
| 试验号 | 碳酸钙(A) | 硫酸铵(B) | 氯化锰(C) | 孢子数/（×109个/g） |
|---|---|---|---|---|
| 1 | -1 | -1 | 1 | 7.05 |
| 2 | 0 | 0 | 0 | 8.40 |
| 3 | 1 | -1 | 1 | 6.40 |
| 4 | 0 | 0 | 0 | 8.40 |
| 5 | 0 | 0 | 1.681793 | 7.97 |
| 6 | -1 | -1 | -1 | 5.95 |
| 7 | -1 | 1 | -1 | 5.72 |
| 8 | -1 | 1 | 1 | 6.72 |
| 9 | 1.681793 | 0 | 0 | 5.60 |
| 10 | 1 | 1 | -1 | 4.50 |
| 11 | 1 | 1 | 1 | 6.06 |
| 12 | 0 | 0 | 0 | 8.40 |
| 13 | 0 | 0 | 0 | 8.40 |
| 14 | 0 | 1.681793 | 0 | 7.02 |
| 15 | 0 | 0 | -1.68179 | 6.98 |
| 16 | 0 | 0 | 0 | 8.40 |
| 17 | 0 | 0 | 0 | 8.40 |
| 18 | 0 | -1.68179 | 0 | 7.30 |
| 19 | 1 | -1 | -1 | 7.39 |
| 20 | -1.68179 | 0 | 0 | 6.27 |
| 变异来源 | 自由度 | 平方和 | 均方值 | F值 | Prob>F |
|---|---|---|---|---|---|
| 模型 | 23.33 | 9 | 2.59 | 11.13 | 0.0004 |
| A-碳酸钙 | 0.36 | 1 | 0.36 | 1.54 | 0.2423 |
| B-硫酸铵 | 1.33 | 1 | 1.33 | 5.70 | 0.0381 |
| C-氯化锰 | 1.37 | 1 | 1.37 | 5.90 | 0.0355 |
| AB | 8.91 | 1 | 8.91 | 3.82 | 0.0790 |
| AC | 2.93 | 1 | 2.93 | 1.26 | 0.2887 |
| BC | 7.50 | 1 | 7.50 | 3.22 | 0.1030 |
| A^2 | 1.40 | 1 | 1.40 | 60.04 | < 0.0001 |
| B^2 | 4.40 | 1 | 4.40 | 18.86 | 0.0015 |
| C^2 | 2.80 | 1 | 2.80 | 12.02 | 0.0061 |
| 残差 | 2.33 | 10 | 2.33 | --- | --- |
| 失拟项 | 2.33 | 5 | 4.67 | --- | --- |
| 纯误差 | 0.00 | 5 | 0.00 | --- | --- |
| 总和 | 25.66 | 19 | --- | --- | |
| R2 | 90.92% | --- | --- | --- | |
| R2Adj | 82.75% | --- | --- | --- |
| 变异来源 | 自由度 | 平方和 | 均方值 | F值 | Prob>F |
|---|---|---|---|---|---|
| 模型 | 23.33 | 9 | 2.59 | 11.13 | 0.0004 |
| A-碳酸钙 | 0.36 | 1 | 0.36 | 1.54 | 0.2423 |
| B-硫酸铵 | 1.33 | 1 | 1.33 | 5.70 | 0.0381 |
| C-氯化锰 | 1.37 | 1 | 1.37 | 5.90 | 0.0355 |
| AB | 8.91 | 1 | 8.91 | 3.82 | 0.0790 |
| AC | 2.93 | 1 | 2.93 | 1.26 | 0.2887 |
| BC | 7.50 | 1 | 7.50 | 3.22 | 0.1030 |
| A^2 | 1.40 | 1 | 1.40 | 60.04 | < 0.0001 |
| B^2 | 4.40 | 1 | 4.40 | 18.86 | 0.0015 |
| C^2 | 2.80 | 1 | 2.80 | 12.02 | 0.0061 |
| 残差 | 2.33 | 10 | 2.33 | --- | --- |
| 失拟项 | 2.33 | 5 | 4.67 | --- | --- |
| 纯误差 | 0.00 | 5 | 0.00 | --- | --- |
| 总和 | 25.66 | 19 | --- | --- | |
| R2 | 90.92% | --- | --- | --- | |
| R2Adj | 82.75% | --- | --- | --- |
| [1] | 王磊, 皇传华, 李秀深, 等. 绿色木霉改良菌株在蔬菜生产中的应用研究[J]. 安徽农业科学, 2010,38(23):12545-12547. |
| [2] | 葛文中, 李楠. 绿色木霉应用的研究进展[J]. 黑龙江八一农垦大学学报, 2005,17(4):75-80. |
| [3] |
Verma M, Brar S K, Tyagi R D, et al. Antagonistic fungi,Trichoderma spp.: Panoply of biological control[J]. Biochemical Engineering Journal, 2007,37(1):1-20.
doi: 10.1016/j.bej.2007.05.012 URL |
| [4] | 王勇, 王万立, 刘春艳, 等. 绿色木霉Tr9701对多种病原菌的抑制作用及其抑病机理[J]. 中国农学通报, 2008,24(1):371-374. |
| [5] | 刘路宁, 屠艳拉, 张敬泽. 绿木霉菌株TY009防治纹枯病等水稻主要真菌病害的潜力[J]. 中国农业科学, 2010,43(10):2031-2038. |
| [6] |
陆楚月, 马艳, 王巧, 等. 绿色木霉TV41对尖孢镰刀菌FW0在西瓜植株空间分布和枯萎病防控效果的影响[J]. 微生物学通报, 2015,42(11):2159-2167.
doi: 10.13344/j.microbiol.china.150405 URL |
| [7] | 辛鑫, 刘磊, 潘江禹, 等. 绿色木H6对香蕉枯萎病的诱导抗性作用[J]. 广东农业科学, 2013,40(7):83-85. |
| [8] | 潘亚妮, 惠有为. 绿色木霉防治温室灰霉病研究[J]. 安徽农业科学, 2010,38(15):7913-7914,7964. |
| [9] | 马娜娜, 马韵升, 张心青, 等. 一株产胶霉菌素菌株的ARTP诱变及筛选[J]. 中国农学通报, 2017,33(29):36-41. |
| [10] | Fravel D R. Role of antibiotics in the biocontrol of plant diseases. Annual Review of Phytopathology, 1988,26:75-91. |
| [11] | 王晓明, 孙玉辉, 张欢, 等. 绿色木霉固态发酵生产纤维素酶条件优化与酶的固定化[J]. 浙江农业学报, 2014,26(1):186-193. |
| [12] | 刘华, 李崇高, 黄建初. 响应面法对绿色木霉产纤维素酶固态发酵条件优化[J]. 安徽农业大学学报, 2017,44(6):980-985. |
| [13] | 武金霞, 武建, 朱晓. 绿色木霉JD-1固态发酵玉米芯产纤维素酶条件优化[J]. 中国酿造, 2015,34(34):79-83. |
| [14] |
Yang Y H, Wang B C, Wang Q H, et al. Research on solid-state fermentation on rice chaff with a microbial consortium[J]. Colloid Surface B, 2004,34(1):1-6.
doi: 10.1016/j.colsurfb.2003.10.009 URL |
| [15] | 肖龙龙, 张崇玉, 付责中. 绿色木霉产孢条件的优化[J]. 山地农业生物学报, 2012,31(1):036-039. |
| [16] | 刘时轮, 李勇, 傅俊范, 等. 绿色木霉菌株Tv04-2固体发酵条件研究[J]. 华北农学报, 2008,23(增刊):244-247. |
| [17] | 李运华, 王晓彬, 李振. 利用固体发酵工艺生产绿色木霉孢子粉的研究[J]. 黑龙江科技信息, 2016,2:229-230. |
| [18] | 田泱源, 李瑞芳. 响应面法在生物过程优化中的应用[J]. 食品工程, 2010,9(2):8-11,53. |
| [19] |
Miller A, Sitter R R. Usingthe folded-over122-run plachett-burman design to consider interactions[J]. Technometrics, 2001,43(1):44-54.
doi: 10.1198/00401700152404318 URL |
| [20] | Montgomery D C. Design and analysis of experiments(3nd ed)[M]. New York :John Wily＆Sons, 1991,32(1):35-41. |
| [21] | 王允祥, 吕风霞, 陆兆新. 杯伞发酵培养基的响应曲面法优化研究[J]. 南京农业大学学报, 2004,27(3):89-94. |
| [22] | 邢欢, 许文宗, 李婕, 等. 绿色木霉自絮凝产纤维素酶发酵条件的优化[J]. 食品工业, 2015,36(6):228-231. |
| [23] | 陈晓萍, 孙付保, 陈晓旭, 等. 响应面法优化康宁木霉产纤维素酶固态发酵培养基[J]. 食品与生物技术学报, 2011,30(1):106-112. |
| [24] | 郭清华, 熊本涛, 陈少林. 粗糙脉孢菌产纤维素酶培养条件的优化[J]. 西北林学院学报. 2018,33(2), 151-155. |
| [25] | 张良, 纪明山术, 张玉芬, 等. 绿色木霉TR-8发酵工艺条件筛选[J]. 沈阳农业大学学报, 2005,36(4):494-496. |
| [26] | 王君君, 谭显东, 羊依金. 三七渣固态发酵生产生防菌绿色木霉的初步研究[J]. 中国酿造, 2012,31(11):62-65. |
| [27] | 张双玺, 张兴. 利用植物农药残渣生产绿色木霉孢子的研究[J]. 西北农林科技大学学报:自然科学版, 2008,36(4):175-180. |
| [28] | 周莲, 谢小林, 吴天福, 等. 淡紫拟青霉M-1固体发酵工艺优化及基于自动化种曲机的中试生产[J]. 中国生物防治学报, 2019,35(5):793-804. |
| [29] | 付跃, 柳雨珠, 韦秋艳, 等. 绿色木霉和米曲霉混合固体发酵产纤维素酶的工艺条件[J]. 贵州农业科学, 2019,47(5):61-65. |
| [30] | 蔡守平. 绿僵菌MaZPTR-01菌株固体发酵条件筛选研究[J]. 西南林业大学学报, 2016,36(5):100-105. |
| [1] | WANG Wenxuan, LI Yunpeng, XU Hening, ZHAO Xin, MA Rou, ZHANG Lina, ZHANG Yingying. Ultrasonic Extraction Process of Total Saponins from Leaves of Solanum torvum Swartz. [J]. Chinese Agricultural Science Bulletin, 2022, 38(30): 118-125. |
| [2] | LIU Lilan, QIU Qinqin, FAN Wenrong, TANG Jiao, HU Xiaobing, XIAO Wei, CHEN Ke. The Removal Process of Organophosphorus Pesticide Residues on the Surface of Commercial Fruits by Alkaline Electrolyzed Water [J]. Chinese Agricultural Science Bulletin, 2022, 38(2): 133-140. |
| [3] | JIANG Wenqiang, MIAO Linghong, GAO Liang, ZHU Yuejie, LIN Yan, LUO Chenhao, QIAN Linjie, CHEN Shiyou, ZHANG Weina, SHI Dalin, LIU Bo, SHEN Huaishun, GE Xianping. Study on Nutrients and Quality Improvement of Liquid Fermented Mulberry Leaf Meal by Bacillus zhangzhouensis Using Response Surface Methodology [J]. Chinese Agricultural Science Bulletin, 2022, 38(16): 145-154. |
| [4] | Xu Dandan, Xu Yaqin, Juan Xing, Zhou Shoubiao, Wang Changbao, Hang Hua. Optimization of Extraction Process of Se-enriched Jerusalem artichoke Polysaccharides and Its Antioxidant Activity [J]. Chinese Agricultural Science Bulletin, 2021, 37(30): 121-127. |
| [5] | Xiao Qinjian, Zhang Xiaohu, Gao Mengdie, Zhang Junwei. Extraction and Separation of Schisandrin B and Its Compound Preservative [J]. Chinese Agricultural Science Bulletin, 2021, 37(12): 126-135. |
| [6] | Pan Dongmei, Yang Chuanlun, Zhang Xinqing, Ni Jianlong, Wang Xiuzhi, Wang Chun. BBD-response Surface Methodology Optimizes the Cultivation Conditions of Producing Gliotoxin by Trichoderma viride [J]. Chinese Agricultural Science Bulletin, 2020, 36(3): 111-116. |
| [7] | Gao Xiang, Fan Xiaoxu, Zhang Shuzhen, Song Fuqiang. The Effect of Herbicide Application on Arbuscular Mycorrhizal Fungi in Farmland: A Review [J]. Chinese Agricultural Science Bulletin, 2020, 36(27): 129-134. |
| [8] | Lin Fan, Hou Xiaoxiao, Liu Jingyu, Chang Mingchang, Meng Junlong. Extraction Technology and Antioxidant Activity of Polysaccharides from Cordyceps militaris Fruiting Body [J]. Chinese Agricultural Science Bulletin, 2020, 36(2): 122-128. |
| [9] | Yang Dandan, Wang Jianping, Ma Nana, Zhang Xinqing, Ni Jianlong, Pan Dongmei. Optimization of Solid-state Fermentation for Spores Production by Trichoderma viride [J]. Chinese Agricultural Science Bulletin, 2020, 36(12): 111-119. |
| [10] | . Optimization of Brown Rice Instant Porridge Processing Technology Based on Response Surface Methodology [J]. Chinese Agricultural Science Bulletin, 2019, 35(8): 106-113. |
| [11] | . Flavonoids from Seeds of Mirabilis jalapa: Extraction Technology Optimized by Response Surface Methodology and Antioxidant Activity [J]. Chinese Agricultural Science Bulletin, 2019, 35(34): 127-133. |
| [12] | . Research Progress on Isolating Microspores Culture of Tomato [J]. Chinese Agricultural Science Bulletin, 2019, 35(30): 42-48. |
| [13] | . Optimization of Suspension Culture Condition for Producing Rosmarinic Acid from Perilla Cells [J]. Chinese Agricultural Science Bulletin, 2019, 35(28): 32-37. |
| [14] | . Wogonin Extraction from Shangluo Scutellaria baicalensis and Application of Its Compound Preservative Solution [J]. Chinese Agricultural Science Bulletin, 2019, 35(22): 147-155. |
| [15] | . Chlorogenic Acid and Rutin from Inferior and Waste Tobacco: Extraction Process [J]. Chinese Agricultural Science Bulletin, 2019, 35(1): 57-62. |
| Viewed | ||||||
|
Full text |
|
|||||
|
Abstract |
|
|||||
