Extraction Technology and Antioxidant Activity of Polysaccharides from Cordyceps militaris Fruiting Body

doi:10.11924/j.issn.1000-6850.casb18120014

Abstract

Abstract:

The paper aims to obtain the optimal condition for extracting polysaccharide from Cordyceps militaris fruiting body by hot water and to provide reference for the study of its immune activity. Taking C. militaris fruiting body as the research object, using the new deproteinization method, based on the single factor experiment and the response surface method, we optimized the extraction process parameters of the polysaccharide of C. militaris fruiting body, and studied the antioxidant activity of the polysaccharide. The optimum extraction condition is: the extraction temperature of 80℃, the extraction time of 3.1 h, the ratio of liquid to material of 41:1 (mL:g), and the extraction of 2 times. Under this condition, the actual yield of polysaccharide is 6.3328%, and the relative error from the predicted value of 6.5877% is less than 5%, indicating that the optimization parameters of extraction process are reliable. The scavenging ability of hydroxyl radical is 53.8%, the reduction ability of iron ion is 1.133 and the total antioxidant activity is 0.807 when the polysaccharide obtained by this method is 8 mg/mL. The scavenging ability of 50% inhibiting concentration is 1.449 mg/mL. The method of extraction of polysaccharide with high extraction rate and high antioxidant activity is obtained, which could lay a foundation for the separation, purification and bioactivity of polysaccharide from C. militaris fruiting body.

Key words: polysaccharide, hot water extraction, response surface methodology, Cordyceps militaris fruiting body, in vitro antioxidant activity

CLC Number:

R284.2

Lin Fan, Hou Xiaoxiao, Liu Jingyu, Chang Mingchang, Meng Junlong. Extraction Technology and Antioxidant Activity of Polysaccharides from Cordyceps militaris Fruiting Body[J]. Chinese Agricultural Science Bulletin, 2020, 36(2): 122-128.

Figures/Tables 12

References 26

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因素	编码水平
因素	-1	0	1
X₁浸提温度	70	80	90
X₂浸提时间	2	3	4
X₃液料比	30:1	40:1	50:1
X₄浸提次数	1	2	3

因素	编码水平
因素	-1	0	1
X₁浸提温度	70	80	90
X₂浸提时间	2	3	4
X₃液料比	30:1	40:1	50:1
X₄浸提次数	1	2	3

来源	平方和	自由度	均差	F	P	显著性	模型系数
模型	3.920	14	0.280	39.11	< 0.0001	^**
X₁	0.028	1	0.028	3.970	0.0695
X₂	0.094	1	0.094	13.10	0.0035	^**
X₃	0.062	1	0.062	8.620	0.0125	^*
X₄	0.770	1	0.770	107.98	< 0.0001	^**
X₁X₂	0.00378	1	0.00378	0.530	0.4811
X₁X₃	0.00141	1	0.00141	0.200	0.6654
X₁X₄	0.000361	1	0.000361	0.050	0.8260
X₂X₃	0.0017	1	0.0017	0.240	0.6345
X₂X₄	0.024	1	0.024	3.360	0.0918
X₃X₄	0.076	1	0.076	10.67	0.0068	^**
X₁²	1.720	1	1.720	240.43	< 0.0001	^**
X₂²	0.820	1	0.820	115.11	< 0.0001	^**
X₃²	1.290	1	1.290	180.66	< 0.0001	^**
X₄²	1.660	1	1.660	232.5	< 0.0001	^**
残差	0.086	12	0.007154
失拟误差	0.064	10	0.0064	0.590	0.7691	不显著
纯误差	0.022	2	0.011
总值	4	26
R²							0.9786
Adj R²							0.9535

来源	平方和	自由度	均差	F	P	显著性	模型系数
模型	3.920	14	0.280	39.11	< 0.0001	^**
X₁	0.028	1	0.028	3.970	0.0695
X₂	0.094	1	0.094	13.10	0.0035	^**
X₃	0.062	1	0.062	8.620	0.0125	^*
X₄	0.770	1	0.770	107.98	< 0.0001	^**
X₁X₂	0.00378	1	0.00378	0.530	0.4811
X₁X₃	0.00141	1	0.00141	0.200	0.6654
X₁X₄	0.000361	1	0.000361	0.050	0.8260
X₂X₃	0.0017	1	0.0017	0.240	0.6345
X₂X₄	0.024	1	0.024	3.360	0.0918
X₃X₄	0.076	1	0.076	10.67	0.0068	^**
X₁²	1.720	1	1.720	240.43	< 0.0001	^**
X₂²	0.820	1	0.820	115.11	< 0.0001	^**
X₃²	1.290	1	1.290	180.66	< 0.0001	^**
X₄²	1.660	1	1.660	232.5	< 0.0001	^**
残差	0.086	12	0.007154
失拟误差	0.064	10	0.0064	0.590	0.7691	不显著
纯误差	0.022	2	0.011
总值	4	26
R²							0.9786
Adj R²							0.9535