Under-forest Tetrastigma hemsleyanum: Polysaccharide Fingerprint in Different Producing Areas

doi:10.11924/j.issn.1000-6850.casb2021-0372

Abstract

Abstract:

The evaluation system of polysaccharide fingerprint was established to identify the source and control the quality of Tetrastigma hemsleyanum. T. hemsleyanum from different areas were used as materials, the polysaccharide spectrum, monosaccharide content and composition of T. hemsleyanum from different areas were determined by PMP pre-column derivatization-HPLC and chemometric analysis. The polysaccharide fingerprint and its common pattern were established for different origins, and 8 monosaccharide chromatographic peaks such as glucose, rhamnose and arabinose were determined. The similarity of each batch of samples was high, ranging from 0.934 to 0.997. According to the score of principal component factor, the quality of polysaccharide from Suichang was the best, followed by that from Wenzhou. The composition of its monosaccharides was determined, and high content of mannose, glucose, galactose and arabinose were obtained, which was 9.094%, 11.451%, 56.695% and 11.030%, respectively, with galactose being the main component of the polysaccharide of T. hemsleyanum. In this study, we established the polysaccharides fingerprint of T. hemsleyanum and determined the composition and content of monosaccharides. The method is stable and reliable, and can be used for the source identification and quality control of T. hemsleyanum.

Key words: Tetrastigma hemsleyanum, polysaccharide, pre-column derivatization, PMP, fingerprints

CLC Number:

S567
R931.5

Zi Haoran, Xu Haishun, Wu Shengnan, Zhang Danning, Xu Juan, Bai Yan, Wang Hongzhen, Wu Xueqian. Under-forest Tetrastigma hemsleyanum: Polysaccharide Fingerprint in Different Producing Areas[J]. Chinese Agricultural Science Bulletin, 2021, 37(27): 139-145.

Figures/Tables 13

References 28

[1]	何文, 李瀚鑫, 王晰雯, 等. 三叶青不同萃取部位抗氧化活性[J]. 食品工业, 2020, 41(2):151-155.
[2]	张晓芹, 刘帅英, 王美红, 等. 畲药三叶青饮片的HPLC指纹图谱研究[J]. 中药材, 2018, 41(09):2139-2142.
[3]	徐硕, 金鹏飞, 徐文峰, 等. 民间中药三叶青的研究进展[J]. 中南药学, 2016, 14(12):1336-1341.
[4]	蔡韦炜, 陈丹, 范世明, 等. 三叶青地上部分化学成分分析[J]. 福建中医药大学学报, 2013, 23(05):34-35,42.
[5]	何伯伟, 姜娟萍, 徐丹彬. 道地药材“浙八味”与新“浙八味”[J]. 新农村, 2020,(05):21-22.
[6]	许海顺, 吴学谦, 熊科辉, 等. 三叶青不同洗脱组分的抗氧化活性研究[J]. 中华中医药学刊, 2015, 33(8):1968-1971,2068.
[7]	Ru Y, Chen X, Wang J, et al. Structural characterization, hypoglycemic effects and mechanism of a novel polysaccharide from Tetrastigma hemsleyanum Diels et Gilg[J]. International Journal of Biological Macromolecules, 2019, 123:775-783. doi: 10.1016/j.ijbiomac.2018.11.085 URL
[8]	Ru Y, Chen X, Wang J, et al. Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg: Extraction optimization, structural characterizations, antioxidant and antihyperlipidemic activities in hyperlipidemic mice[J]. International Journal of Biological Macromolecules, 2019, 125:1033-1041. doi: 10.1016/j.ijbiomac.2018.11.236 URL
[9]	郭南生, 江和栋, 张兵, 等. 三叶青根多糖提取工艺优化、分离纯化及结构表征[J]. 食品与机械, 2018, 34(07):143-147,210.
[10]	黄有强. 微波辅助提取三叶青多糖工艺优化及其拮抗炎症细胞的研究[J]. 浙江中医杂志, 2017, 52(05):384-385.
[11]	饶君凤, 吕伟德, 倪承珠, 等. 离子色谱法测定三叶青多糖中单糖组成[J]. 亚太传统医药, 2016, 12(12):42-44.
[12]	吕兰, 陈红梅, 吴敏. 三叶青多糖提取工艺优化及单糖组成分析[J]. 南方农业, 2020, 14(01):6-10.
[13]	马丹丹, 李伟平, 马哲龙, 等. 三叶青多糖抗肝损伤作用的研究[J]. 医学研究杂志, 2012, 41(01):33-36.
[14]	韩邦兴, 陈凌霄, 邓勇, 等. 糖谱法结合多元色谱分析比较铁皮石斛功能性多糖结构特征[J]. 药物分析杂志, 2018, 38(01):41-49.
[15]	周勇, 易延逵, 杨晓敏, 等. 香菇中多糖含量测定方法的比较研究[J]. 食品研究与开发, 2016, 37(13):124-128.
[16]	黄小兰, 何旭峰, 杨勤, 等. PMP柱前衍生化HPLC法测定地参多糖的单糖组成[J]. 食品与发酵工业, 2020, 46(07):250-256.
[17]	周彦强, 吴光斌, 陈发河. PMP柱前衍生化HPLC法测定黄秋葵多糖的单糖组成[J]. 食品科学, 2019, 40(04):266-271.
[18]	万强, 吴学昊, 范华均, 等. HPLC衍生化法分析决明子多糖水解产物中单糖组分及其多糖组成特征的研究[J]. 分析测试学报, 2014, 33(11):1231-1236.
[19]	陈两绵, 高慧敏, 朱晶晶, 等. 不同采收及加工方式对穿心莲药材质量的影响[J]. 中国中药杂志, 2020, 45(8):1717-1725.
[20]	聂韡, 朱培林, 房海灵, 等. HPLC指纹图谱结合化学计量学评价不同产地广东紫珠药材的质量[J]. 中草药, 2017, 48(01):185-191.
[21]	林林, 于凤蕊, 徐丽华, 等. 女金丸HPLC指纹图谱建立及聚类分析和主成分分析[J]. 中国药房, 2019, 30(10):1339-1343.
[22]	李绍平, 吴定涛, 赵静. 糖谱及其在中药多糖质量控制中的应用[J]. 中国中药杂志, 2015, 40(17):3505-3513.
[23]	郭菁菁, 赵英杰, 李伟, 等. 三叶青地上部分多糖对乳腺癌小鼠的抗肿瘤作用[J]. 中成药, 2019, 41(04):916-919.
[24]	Zhu B, Qian C, Zhou F, et al. Antipyretic and antitumor effects of a purified polysaccharide from aerial parts of Tetrastigma hemsleyanum[J]. Journal of Ethnopharmacology, 2020, 253:112663. doi: 10.1016/j.jep.2020.112663 URL
[25]	Yi R, Xiao C, Jie X, et al. Hypoglycemic effects of a polysaccharide from Tetrastigma hemsleyanum diels et gilg in alloxan-induced diabetic mice[J]. Chemistry & Biodiversity, 2018, 132:765-773.
[26]	Hu D J, Cheong K L, Zhao J, et al. Chromatography in characterization of polysaccharides from medicinal plants and fungi[J]. Journal of Separation Science, 2013, 36(1):1-19. doi: 10.1002/jssc.201200874 pmid: 23225747
[27]	杜泽飞, 陶爱恩, 夏从龙, 等. 基于PMP-HPLC和化学计量学的黄精基原物种多糖差异分析[J]. 中国实验方剂学杂志, 2019, 25(15):25-29.
[28]	李运, 邱国玉, 李晓春, 等. 基于主成分分析的前胡类药材HPLC特征图谱研究[J]. 药物分析杂志, 2019, 39(07):1323-1329.

编号	来源	编号	来源
S1	浙江临安	S6	浙江温州
S2	浙江遂昌	S7	贵州毕节
S3	浙江淳安	S8	贵州都匀
S4	浙江宁波	S9	广西桂林
S5	浙江台州	S10	广西河池

编号	来源	编号	来源
S1	浙江临安	S6	浙江温州
S2	浙江遂昌	S7	贵州毕节
S3	浙江淳安	S8	贵州都匀
S4	浙江宁波	S9	广西桂林
S5	浙江台州	S10	广西河池

样品	含量/(mg/g)	RSD/%	纯度/%	样品	含量/(mg/g)	RSD/%	纯度/%
S1	29.030	0.872	83.42	S6	32.926	0.561	74.05
S2	35.586	0.901	87.36	S7	19.021	1.105	82.67
S3	18.182	0.758	80.11	S8	22.528	0.678	80.42
S4	17.005	0.350	78.93	S9	15.081	0.395	86.13
S5	24.895	0.076	73.83	S10	20.015	0.797	75.28

样品	含量/(mg/g)	RSD/%	纯度/%	样品	含量/(mg/g)	RSD/%	纯度/%
S1	29.030	0.872	83.42	S6	32.926	0.561	74.05
S2	35.586	0.901	87.36	S7	19.021	1.105	82.67
S3	18.182	0.758	80.11	S8	22.528	0.678	80.42
S4	17.005	0.350	78.93	S9	15.081	0.395	86.13
S5	24.895	0.076	73.83	S10	20.015	0.797	75.28

主成分因子	特征值	方差贡献率/%	累积方差贡献率/%
1	3.476	43.452	43.452
2	2.893	36.166	79.618