Abstract:

To enrich the technical parameters of Ganoderma lucidum cultivation with mulberry chips and determine the optimum particle size of mulberry chips, the G. lucidum cultivar ‘Chuanyuanzhi No.1' was cultivated with different-particle-size mulberry chips (20, 18, 14, 12 and 8 mm) in bags and the mycelial growth rate, morphological characters and active components of the fruiting bodies and yield of different treatments were compared in this study. The substrates consisted of 45% mulberry chips, 45% corncob, 5% bran, 4% corn flour and 1% gypsum. Results showed that the mycelia grew fastest in the substrates with medium-particle-size mulberry chips (8-14 mm) with the growth rate of 6.27 mm/d. However, G. lucidum cultivated with 18 mm-particle-size mulberry chips (D18) had the biggest fresh single weight (95.59 g) and yield (34.10 g/bag). In addition, treatment D18 had higher content of triterpenoid acid and polysaccharide in the fruiting bodies, reaching 1.33 and 1.20 g/100 g, respectively. Conclusively, G. lucidum cultivated with 18 mm-particle-size mulberry chips had higher yield and better quality.

Key words: mulberry chips, particle size, Ganoderma lucidum, yield, triterpenoid acid, polysaccharide