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Chinese Agricultural Science Bulletin ›› 2021, Vol. 37 ›› Issue (27): 132-138.doi: 10.11924/j.issn.1000-6850.casb2020-0729

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Optimization of Ultrasonic Extraction Process of Red Pigment from ‘Carmine Radish’ by Orthogonal Method

Peng Hua1,2(), Song Xia2, Zhang Chun3()   

  1. 1Department of Tourism and Cultural Industry, Sichuan Tourism University, Chengdu 610100
    2Research Center for Tourism Agricultural Development, Sichuan Tourism University, Chengdu 610100
    3Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou Sichuan 646000
  • Received:2020-12-08 Revised:2021-05-10 Online:2021-09-25 Published:2021-10-28
  • Contact: Zhang Chun E-mail:penghua623@foxmail.com;zc83good@126.com

Abstract:

To optimize the extraction process of red pigment from ‘carmine radish’, the ‘Yanzhihong radish 2’ was used as the raw material to analyze the red pigment extraction yield under different solvents by using ultrasonic technology, and to study the effects of different types and ratios of extraction liquid, different solid-liquid ratios, extraction temperature and extraction time on the absorbance value. The results showed that: (1) the absorbance value of the red pigment solution extracted by acetone hydrochloride was the highest; (2) the effect of extraction liquid ratio on the absorbance value was significant, and the effects of solid-liquid ratio and extraction temperature on the absorbance value were extremely significant; the effects of extraction temperature, solid-liquid ratio and the ratio of extraction solution on the absorbance value were in a descending order; (3) the content of red pigment in different radish varieties were measured by the most optimal extraction condition, showing that the absorbance value of the red pigment extract of ‘Yanzhihong radish 1’ and ‘Yanzhihong radish 2’ were the highest. In general, the absorbance value of red pigment extraction is the highest under the condition of using acetone hydrochloride as the extracting solution, the ratio of 10.8% hydrochloric acid: 30% acetone is 1:1, the solid-liquid ratio is 1:8 (g/mL), the extraction temperature is 50℃ and the extracting duration is 20 min.

Key words: ‘carmine radish’, red pigment, ultrasonic extraction, orthogonal design method, absorbance value

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