Chinese Agricultural Science Bulletin ›› 2021, Vol. 37 ›› Issue (27): 132-138.doi: 10.11924/j.issn.1000-6850.casb2020-0729
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Peng Hua1,2(), Song Xia2, Zhang Chun3(
)
Received:
2020-12-08
Revised:
2021-05-10
Online:
2021-09-25
Published:
2021-10-28
Contact:
Zhang Chun
E-mail:penghua623@foxmail.com;zc83good@126.com
CLC Number:
Peng Hua, Song Xia, Zhang Chun. Optimization of Ultrasonic Extraction Process of Red Pigment from ‘Carmine Radish’ by Orthogonal Method[J]. Chinese Agricultural Science Bulletin, 2021, 37(27): 132-138.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb2020-0729
仪器名称 | 型号 | 生产厂家 |
---|---|---|
电热恒温干燥箱 | 202 A-2 | 北京中兴伟业仪器有限公司 |
离心机 | ST40 | 美国赛默飞世尔科技公司 |
粉碎机 | XFB-200 | 湖南中诚制药机械厂 |
电子天平 | FA 2204 B | 上海超平科学仪器有限公司 |
分光光度计 | UV2600i | 岛津仪器(苏州)有限公司 |
超声波清洗机 | JM-10D-45 | 深圳市洁盟清洗设备有限公司 |
仪器名称 | 型号 | 生产厂家 |
---|---|---|
电热恒温干燥箱 | 202 A-2 | 北京中兴伟业仪器有限公司 |
离心机 | ST40 | 美国赛默飞世尔科技公司 |
粉碎机 | XFB-200 | 湖南中诚制药机械厂 |
电子天平 | FA 2204 B | 上海超平科学仪器有限公司 |
分光光度计 | UV2600i | 岛津仪器(苏州)有限公司 |
超声波清洗机 | JM-10D-45 | 深圳市洁盟清洗设备有限公司 |
试验号 | 料液比/(g/mL) | 提取温度/℃ | 料液比×温度 | 提取液配比 | 料液比×提取液配比 | 吸光值Ⅰ | 吸光值Ⅱ | 吸光值Ⅲ |
---|---|---|---|---|---|---|---|---|
1 | 1:4 | 30 | 1 | 3:7 | 1 | 1.494 | 1.530 | 1.329 |
2 | 1:4 | 40 | 2 | 4:6 | 2 | 1.655 | 1.497 | 1.769 |
3 | 1:4 | 50 | 3 | 5:5 | 3 | 1.915 | 1.919 | 2.077 |
4 | 1:4 | 60 | 4 | 6:4 | 4 | 1.296 | 1.427 | 1.426 |
5 | 1:6 | 30 | 2 | 5:5 | 4 | 1.804 | 1.890 | 1.656 |
6 | 1:6 | 40 | 1 | 6:4 | 3 | 1.975 | 1.861 | 2.080 |
7 | 1:6 | 50 | 4 | 3:7 | 2 | 2.287 | 2.141 | 2.394 |
8 | 1:6 | 60 | 3 | 4:6 | 1 | 1.328 | 1.257 | 1.462 |
9 | 1:8 | 30 | 3 | 6:4 | 2 | 1.993 | 1.916 | 1.837 |
10 | 1:8 | 40 | 4 | 5:5 | 1 | 2.231 | 2.206 | 2.297 |
11 | 1:8 | 50 | 1 | 4:6 | 4 | 2.27 | 2.197 | 2.228 |
12 | 1:8 | 60 | 2 | 3:7 | 3 | 1.379 | 1.235 | 1.412 |
13 | 1:10 | 30 | 4 | 4:6 | 3 | 1.914 | 1.991 | 1.870 |
14 | 1:10 | 40 | 3 | 3:7 | 4 | 1.887 | 2.003 | 1.781 |
15 | 1:10 | 50 | 2 | 6:4 | 1 | 2.114 | 2.128 | 1.957 |
16 | 1:10 | 60 | 1 | 5:5 | 2 | 1.334 | 1.427 | 1.323 |
试验号 | 料液比/(g/mL) | 提取温度/℃ | 料液比×温度 | 提取液配比 | 料液比×提取液配比 | 吸光值Ⅰ | 吸光值Ⅱ | 吸光值Ⅲ |
---|---|---|---|---|---|---|---|---|
1 | 1:4 | 30 | 1 | 3:7 | 1 | 1.494 | 1.530 | 1.329 |
2 | 1:4 | 40 | 2 | 4:6 | 2 | 1.655 | 1.497 | 1.769 |
3 | 1:4 | 50 | 3 | 5:5 | 3 | 1.915 | 1.919 | 2.077 |
4 | 1:4 | 60 | 4 | 6:4 | 4 | 1.296 | 1.427 | 1.426 |
5 | 1:6 | 30 | 2 | 5:5 | 4 | 1.804 | 1.890 | 1.656 |
6 | 1:6 | 40 | 1 | 6:4 | 3 | 1.975 | 1.861 | 2.080 |
7 | 1:6 | 50 | 4 | 3:7 | 2 | 2.287 | 2.141 | 2.394 |
8 | 1:6 | 60 | 3 | 4:6 | 1 | 1.328 | 1.257 | 1.462 |
9 | 1:8 | 30 | 3 | 6:4 | 2 | 1.993 | 1.916 | 1.837 |
10 | 1:8 | 40 | 4 | 5:5 | 1 | 2.231 | 2.206 | 2.297 |
11 | 1:8 | 50 | 1 | 4:6 | 4 | 2.27 | 2.197 | 2.228 |
12 | 1:8 | 60 | 2 | 3:7 | 3 | 1.379 | 1.235 | 1.412 |
13 | 1:10 | 30 | 4 | 4:6 | 3 | 1.914 | 1.991 | 1.870 |
14 | 1:10 | 40 | 3 | 3:7 | 4 | 1.887 | 2.003 | 1.781 |
15 | 1:10 | 50 | 2 | 6:4 | 1 | 2.114 | 2.128 | 1.957 |
16 | 1:10 | 60 | 1 | 5:5 | 2 | 1.334 | 1.427 | 1.323 |
变异来源 | 平方和 | 自由度 | 均方 | F值 |
---|---|---|---|---|
料液比 | 0.6666 | 3 | 0.2222 | 24.6869** |
提取温度 | 3.9244 | 3 | 1.3081 | 145.3285** |
料液比×温度 | 0.4258 | 3 | 0.1419 | 15.7689** |
提取液配比 | 0.0794 | 3 | 0.0265 | 2.9407* |
料液比×提取液配比 | 0.0119 | 3 | 0.004 | 0.4418 |
误差 | 0.288 | 32 | 0.009 |
变异来源 | 平方和 | 自由度 | 均方 | F值 |
---|---|---|---|---|
料液比 | 0.6666 | 3 | 0.2222 | 24.6869** |
提取温度 | 3.9244 | 3 | 1.3081 | 145.3285** |
料液比×温度 | 0.4258 | 3 | 0.1419 | 15.7689** |
提取液配比 | 0.0794 | 3 | 0.0265 | 2.9407* |
料液比×提取液配比 | 0.0119 | 3 | 0.004 | 0.4418 |
误差 | 0.288 | 32 | 0.009 |
因子 | 水平1 | 水平2 | 水平3 | 水平4 |
---|---|---|---|---|
料液比 | 1.6114 | 1.8449 | 1.9336 | 1.8107 |
提取温度 | 1.7689 | 1.937 | 2.1358 | 1.3589 |
料液比×温度 | 1.7542 | 1.7082 | 1.7813 | 1.9569 |
提取液配比 | 1.7395 | 1.7866 | 1.8401 | 1.8343 |
料液比×提取液配比 | 1.7779 | 1.7979 | 1.8024 | 1.8223 |
因子 | 水平1 | 水平2 | 水平3 | 水平4 |
---|---|---|---|---|
料液比 | 1.6114 | 1.8449 | 1.9336 | 1.8107 |
提取温度 | 1.7689 | 1.937 | 2.1358 | 1.3589 |
料液比×温度 | 1.7542 | 1.7082 | 1.7813 | 1.9569 |
提取液配比 | 1.7395 | 1.7866 | 1.8401 | 1.8343 |
料液比×提取液配比 | 1.7779 | 1.7979 | 1.8024 | 1.8223 |
因子 | 极小值 | 极大值 | 极差R | 调整R' |
---|---|---|---|---|
料液比 | 1.6114 | 1.9336 | 0.3222 | 0.29 |
提取温度 | 1.3589 | 2.1358 | 0.7769 | 0.6992 |
料液比×温度 | 1.7082 | 1.9569 | 0.2487 | 0.2238 |
提取液配比 | 1.7395 | 1.8401 | 0.1005 | 0.0905 |
料液比×提取液配比 | 1.7779 | 1.8223 | 0.0444 | 0.0399 |
因子 | 极小值 | 极大值 | 极差R | 调整R' |
---|---|---|---|---|
料液比 | 1.6114 | 1.9336 | 0.3222 | 0.29 |
提取温度 | 1.3589 | 2.1358 | 0.7769 | 0.6992 |
料液比×温度 | 1.7082 | 1.9569 | 0.2487 | 0.2238 |
提取液配比 | 1.7395 | 1.8401 | 0.1005 | 0.0905 |
料液比×提取液配比 | 1.7779 | 1.8223 | 0.0444 | 0.0399 |
品种 | 吸光值 | |
---|---|---|
萝卜根皮 | 萝卜肉质根 | |
罐罐萝卜 | 2.656±0.013 c B | 2.432±0.035 B b |
胭脂红1号 | 2.953±0.047 b A | 2.812±0.022 a A |
胭脂红2号 | 3.041±0.073 a A | 2.842±0.040 A a |
大红袍 | 0.676±0.010 e D | 0.421±0.012 D d |
红心1号 | 2.381±0.049 d C | 2.297±0.008 c C |
西昌白萝卜 | 0.158±0.025 f E | 0.145±0.008 f F |
野生型萝卜 | 0.585±0.029 f D | 0.245±0.021 e E |
品种 | 吸光值 | |
---|---|---|
萝卜根皮 | 萝卜肉质根 | |
罐罐萝卜 | 2.656±0.013 c B | 2.432±0.035 B b |
胭脂红1号 | 2.953±0.047 b A | 2.812±0.022 a A |
胭脂红2号 | 3.041±0.073 a A | 2.842±0.040 A a |
大红袍 | 0.676±0.010 e D | 0.421±0.012 D d |
红心1号 | 2.381±0.049 d C | 2.297±0.008 c C |
西昌白萝卜 | 0.158±0.025 f E | 0.145±0.008 f F |
野生型萝卜 | 0.585±0.029 f D | 0.245±0.021 e E |
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