Antifungal Activity Against Rhizoctonia solani: Mixtures of Phenolic Monoterpenes and Difenoconazole or Thifluzamide

doi:10.11924/j.issn.1000-6850.casb18100030

Abstract

Abstract:

The aim is to explore the control effect of the mixtures of phenolic monoterpenes and fungicides against Rhizoctonia solani and screen new fungicides to control corn sheath blight and rice sheath blight. In this study, thymol and carvacrol were mixed with difenoconazole and thifluzamide respectively, and the in vitro antifungal activity of the mixtures against R. solani was determined by mycelium growth rate method. Meanwhile, the synergistic effects of the mixture of phenolic monoterpenes with fungicides were evaluated according to Sun-Yunpei’s method. The results showed that there was synergism between phenolic monoterpenes and thifluzamide in ratios of 1:5, 1:4, 2:3, 3:2, 4:1 and 5:1. The carvacrol and thifluzamide mixture in the ratio of 4:1 exhibited the highest antifungal activity among all of the mixtures, and the EC₅₀ value and CTC value was 0.928 mg/L and 1397.39, respectively. Similarly, the carvacrol and difenoconazole mixtures in the ratio of 3:2, 4:1 and 5:1, as well as thymol and difenoconazole mixtures in the ratio of 2:3, 4:1 and 5:1, exhibited significant synergistic effect. Thymol and carvacrol could significantly enhance the antifungal activity of difenoconazole or thifluzamide. The carvacrol and thifluzamide mixture in the ratio of 4:1 exhibited high antifungal activity against R. solani, and this mixture could be potentially used to control corn sheath blight and rice sheath blight. When mixed with some fungicides at a certain ratio, the two natural phenolic monoterpenes could significantly enhance their antifungal activity, which has a high research value to the development and screening of fungicide.

Key words: carvacrol, thymol, synergistic effect, Rhizoctonia solani

CLC Number:

S482.2

Wang Kaibo, Wu Wenwei, Wang Qun, Yin Mei, Zhang Xingfu, Yang Congdang, Fu Zhaocong, Li Qiongxian, Pu Jun, Huang Jimei, He Chengxing. Antifungal Activity Against Rhizoctonia solani: Mixtures of Phenolic Monoterpenes and Difenoconazole or Thifluzamide[J]. Chinese Agricultural Science Bulletin, 2020, 36(8): 80-84.

Figures/Tables 1

References 28

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供试药剂*	毒力回归方程	EC₅₀值/(mg/L)	置信区间/(mg/L)	ATI	TTI	CTC
苯醚甲环唑(D)	y=0.704x-0.308	3.466	2.274-5.049	100.00
噻呋酰胺(F)	y=1.846x-1.299	5.058	2.957-10.018	68.53
百里香酚(T)	y=1.945x-2.390	16.94	13.546-23.231	20.46
香芹酚(C)	y=1.993x-2.647	21.292	16.476-31.286	16.28
C:D 1:5	y=1.033x-0.741	5.206	4.038-6.945	66.58	86.05	77.37
C:D 1:4	y=1.036x-0.745	5.342	4.146-7.143	64.88	83.26	77.93
C:D 2:3	y=0.983x-0.656	4.647	3.552-6.208	74.59	66.51	112.14
C:D 3:2	y=0.879x-0.581	4.576	3.389-6.336	75.74	49.77	152.20
C:D 4:1	y=1.175x-0.725	4.138	3.287-5.226	83.76	33.02	253.64
C:D 5:1	y=1.246x-0.805	5.009	3.563-2.783	69.20	30.23	228.88
C:F 1:5	y=1.344x-0.100	1.188	0.813-1.552	291.75	59.82	487.74
C:F 1:4	y=1.598x-0.075	1.114	0.801-1.414	311.13	58.08	535.73
C:F 2:3	y=1.301x-0.138	1.276	0.878-1.664	271.63	47.63	570.33
C:F 3:2	y=1.252x-0.102	1.206	0.804-1.598	287.40	37.18	773.05
C:F 4:1	y=1.009x+0.033	0.928	0.498-1.358	373.49	26.73	1397.39
C:F 5:1	y=1.224x-0.013	1.025	0.644-1.398	338.15	24.99	1353.33
T:D 1:5	y=1.009x-1.070	11.488	8.361-18.518	30.17	86.74	34.78
T:D 1:4	y=0.993x-0.973	9.546	7.077-14.700	36.31	84.09	43.18
T:D 2:3	y=0.857x-0.512	3.954	2.873-5.431	87.66	68.18	128.56
T:D 3:2	y=1.815x-1.472	6.476	5.524-7.738	53.52	52.28	102.38
T:D 4:1	y=1.301x-0.863	4.602	3.744-5.721	75.32	36.37	207.09
T:D 5:1	y=1.437x-1.005	5.009	2.714-11.157	69.20	33.72	205.22
T:F 1:5	y=1.296x-0.494	2.406	1.865-2.976	144.06	60.51	238.05
T:F 1:4	y=1.313x-0.573	2.732	2.157-3.357	126.87	58.91	215.35
T:F 2:3	y=1.295x-0.478	2.341	1.805-2.901	148.06	49.30	300.32
T:F 3:2	y=1.421x-0.661	2.918	2.363-3.533	118.78	39.69	299.30
T:F 4:1	y=1.593x-0.724	2.846	2.351-3.386	121.78	30.07	404.96
T:F 5:1	y=1.579x-0.737	2.929	2.420-3.488	118.33	28.47	415.63

供试药剂*	毒力回归方程	EC₅₀值/(mg/L)	置信区间/(mg/L)	ATI	TTI	CTC
苯醚甲环唑(D)	y=0.704x-0.308	3.466	2.274-5.049	100.00
噻呋酰胺(F)	y=1.846x-1.299	5.058	2.957-10.018	68.53
百里香酚(T)	y=1.945x-2.390	16.94	13.546-23.231	20.46
香芹酚(C)	y=1.993x-2.647	21.292	16.476-31.286	16.28
C:D 1:5	y=1.033x-0.741	5.206	4.038-6.945	66.58	86.05	77.37
C:D 1:4	y=1.036x-0.745	5.342	4.146-7.143	64.88	83.26	77.93
C:D 2:3	y=0.983x-0.656	4.647	3.552-6.208	74.59	66.51	112.14
C:D 3:2	y=0.879x-0.581	4.576	3.389-6.336	75.74	49.77	152.20
C:D 4:1	y=1.175x-0.725	4.138	3.287-5.226	83.76	33.02	253.64
C:D 5:1	y=1.246x-0.805	5.009	3.563-2.783	69.20	30.23	228.88
C:F 1:5	y=1.344x-0.100	1.188	0.813-1.552	291.75	59.82	487.74
C:F 1:4	y=1.598x-0.075	1.114	0.801-1.414	311.13	58.08	535.73
C:F 2:3	y=1.301x-0.138	1.276	0.878-1.664	271.63	47.63	570.33
C:F 3:2	y=1.252x-0.102	1.206	0.804-1.598	287.40	37.18	773.05
C:F 4:1	y=1.009x+0.033	0.928	0.498-1.358	373.49	26.73	1397.39
C:F 5:1	y=1.224x-0.013	1.025	0.644-1.398	338.15	24.99	1353.33
T:D 1:5	y=1.009x-1.070	11.488	8.361-18.518	30.17	86.74	34.78
T:D 1:4	y=0.993x-0.973	9.546	7.077-14.700	36.31	84.09	43.18
T:D 2:3	y=0.857x-0.512	3.954	2.873-5.431	87.66	68.18	128.56
T:D 3:2	y=1.815x-1.472	6.476	5.524-7.738	53.52	52.28	102.38
T:D 4:1	y=1.301x-0.863	4.602	3.744-5.721	75.32	36.37	207.09
T:D 5:1	y=1.437x-1.005	5.009	2.714-11.157	69.20	33.72	205.22
T:F 1:5	y=1.296x-0.494	2.406	1.865-2.976	144.06	60.51	238.05
T:F 1:4	y=1.313x-0.573	2.732	2.157-3.357	126.87	58.91	215.35
T:F 2:3	y=1.295x-0.478	2.341	1.805-2.901	148.06	49.30	300.32
T:F 3:2	y=1.421x-0.661	2.918	2.363-3.533	118.78	39.69	299.30
T:F 4:1	y=1.593x-0.724	2.846	2.351-3.386	121.78	30.07	404.96
T:F 5:1	y=1.579x-0.737	2.929	2.420-3.488	118.33	28.47	415.63