Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2019, Vol. 35 ›› Issue (35): 59-66.doi: 10.11924/j.issn.1000-6850.casb18100074

Special Issue: 生物技术 油料作物 园艺

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Tissue Culture and Regeneration Study of Various Explants of Brassica napus

  

  • Received:2018-10-22 Revised:2019-11-18 Accepted:2019-03-01 Online:2019-12-16 Published:2019-12-16

Abstract: The lateral bud, flower bud receptacle, hypocotyl and cotyledon derived from sterile seedlings of elite Brassica napus restorer line 627R, 621R and 616R were used as explants for tissue culture. The research explored the effects of different seedling ages, pre- culture time, different hormone ratios of pre- culture medium, callus differentiation medium, shoot induced medium and shoot growth medium on shoot regeneration and plant growth vigor. The high frequency of shoot regeneration resulted from hypocotyl and cotyledon derived from 6 days growth seedlings cultured in the following procedure, after 3 days in pre-culture medium (MS+ 2.0 mg/L 2,4-D+ 1.0 mg/L 6-BA+ 30 g/L sucrose+ 8 g/L agar, pH 5.85, the explants were transferred to callus differentiation medium, MS+ 3 mg/L 6-BA+ 1.0 mg/L NAA+ 5 mg/L AgNO3+ 30 g/L sucrose+ 8 g/L agar, pH 5.85, or MS+ 3 mg/L 6-BA+ 1.0 mg/L IAA+ 5 mg/L AgNO3+ 30 g/L sucrose+ 8 g/L agar, pH 5.85. The high frequency of shoot regeneration derived from lateral and buds in the flower bud development could be cultured 30 days in callus or shoot regeneration medium(MS+ 10 mg/L 6-BA+ 1 mg/L NAA+ 30 g/L sucrose+ 8 g/L agar,pH 5.85). All the plants from explants culture had abundant fine roots such as increased root hair number and length, and excellent seedling vigor after transferring to medium with 15 mg/L CCC (MS+ 15 mg/L CCC+ 15-20 g/L sucrose+ 8 g/L agar,pH 5.85) for 30 days. The tissue culture system established for different explants of Brassica napus restorer line will accelerate the reproduction and evaluation of rapeseed varieties.