Effects on Photosynthetic Capacity and Stomatal Opening of Maize Leaves: Exogenous ABA Under Drought Stress

doi:10.11924/j.issn.1000-6850.casb18110062

Abstract

Abstract: This study is to investigate the effects of exogenous ABA on photosynthesis and stomatal opening of maize leaves under drought stress. The excellent maize variety ‘Zhengdan 988’ was used as the material, the changes of photosynthesis, stomatal opening and chlorophyll content of maize seedlings at different time points were studied under PEG treatment, exogenous ABA treatment and ABA+PEG treatment. The results showed that the photosynthesis rate, transpiration rate, stomatal conductance and chlorophyll content of the three treatments were lower than that of CK, and the number of stomatal closure was higher than that of CK. The four indicators of ABA+PEG treatment were between PEG and ABA treatment which indicated that maize reduced transpiration by closing the stomata in order to maintain the growth and development under drought stress. Exogenous ABA can increase the number of stomatal closure and chlorophyll content under drought stress, which reduce water loss and increase the photosynthesis rate of plants under drought stress, and finally improve the drought resistance of the plants.

References

1 Balla K, Bencze S, Janda T, Veisz O. 2009. Analysis of heat stress tolerance in winter wheat. Acta Agron Hung, 57:437-444.
2 Zhuo Y, Zhang YF, Xie GH, Xiong SJ. 2015. Effects of salt stress on biomass and ash composition of switchgrass (Panicum virgatum). Acta Agriculture Scandinavica Section B-soil and Plant Science,
65(4):300-309.
3 Peeva V, Cornic G. 2009. Leaf photosynthesis of Haberlea rhodopensis before and during drought. Environmental and Experimental Botany, 65:310-318.
4 Tingting X, Peixi S U, Lishan S. 2010. Photosynthetic characteristics and water use efficiency of sweet sorghum under different watering regimes. Pakistan Journal of Botany, 42:3981-3994.
5 单长卷, 郝文芳, 张慧成. 2006. 土壤干旱对冬小麦幼苗生理特性的影响. 河北农业大学学报, 29(4):6-18.
6 Hartung W, Sauter A, Hose E. 2002. Abscisic acid in the xylem: where does it come from, where does it go to? Journal of Experimental Botany, 53:27-32.
7 Schachtman DP, Goodger JQD. 2008. Chemical root to shoot signaling under drought. Trends in Plant Science, 13:281-287.
8 Liming X, Jian-Kang Z. Regulation of abscisic acid biosynthesis.[J]. Journal of Biological Chemistry, 2003, 133(1): 29-36.
9 ANDERSON B E, WARD J M, SCHROEDER J I .Evidence for an extracelluar reception site for abscisic acid in commelia guard cells[ J] .Plant Physiol , 1994 , 104:1177-1183.
10 王业遴, 汪良驹, 刘友良.脱落酸提高无花果耐盐性效应(简报)[ J] .植物生理学通讯, 1990,(5):21-23.
11 刘金刚, 沈秀瑛, 张烈, 等.外源脱落酸对玉米抗旱性影响初探[ J] .国外农学-杂粮作物, 1997 ,(6):32 -35.
12 王国英, 刘东臣, 赵紫娟, 等.不同形态氮素及ABA 和6-BA 对烟草生长、养分吸收及其在体内分配的影响[ J] .河北农业大学学报, 2002, 25(2):21 -25 .
13 周碧燕, 李宇彬, 陈杰忠, 等.低温胁迫和喷施ABA 对荔枝内源激素和成花的影响[ J] .园艺学报, 2002, 29(6):577 -578 .
14 王玮, 张枫, 李德全. 外源ABA对渗透胁迫下玉米幼苗根系渗透调节的影响[J]. 作物学报, 2002, 28(1): 121-126.
15 Castro Neto M T D. EFFECT OF WATER DEFICIT ON THE TRANSPIRATION AND STOMATAL RESISTANCE OF MANGO TREE[J]. Revista Brasileira De Fruticultura, 2003, 25(1): 93-95.
16 王会肖, 刘昌明. 作物水分利用效率内涵及研究进展[J]. 水科学进展, 2000, 11(1): 99-104.
17 Wang PT, Song CP. 2008. Guard–cell signalling for hydrogen peroxide and abscisic acid. New Phytologist, 178:703–718.
18 Fu QS, Yang RC, Wang HS, Zhao B, Zhou CL, Ren SX. Guo YD. 2013. Leaf morphological and ultrastructural performance of eggplant (Solanum melongena L.) in response to water stress. Photosynthetica, 51(1):109-114.
19 Pearce DW, Millard S, Bray DF, Rood SB. 2005. Stomatal characteristics of riparian poplar species in a semi-arid environment. Tree physiology, 26:211-218.
20 田琳,谢晓金,包云轩等.不同生育期水分胁迫对夏玉米叶片光合生理特征的影响[J].中国农业气象,2013,34(6):655-660.
21 卜令铎,张仁和,常宇,等.苗期玉米光合特征对水分胁迫的反应[J].生态学报,2010,30(5):1184-1191.
22 Jiang M, Zhang J. Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves[J]. Journal of experimental botany, 2002, 53(379): 2401-2410
23 Souza T C, Magalh?es P C, Castro E M D, Marabesi M A. The influence of ABA on water relation, photosynthesis parameters, and chlorophyll fluorescence under drought conditions in two maize hybrids with contrasting drought resistance[J]. Acta physiologiae plantarum, 2013, 35(2): 515-527
24 鲁晓民,曹丽茹,张新等.PEG胁迫下玉米自交系苗期抗旱性鉴定及评价[J].河南农业科学,2017,4(5)39-44.
25 Yozo N, Keiichi I, Tomokazu K, Yoshihiro O. Effect of aba upon anthocyanin synthesis in regenerated torenia shoots.Journal of Plant Research, 2006, 119(2): 137-144.
26 Teng K, Li J, Lei L, Han Y, Du Y, Jing Z. Exogenous aba induces drought tolerance in upland rice: the role of chloroplast and aba biosynthesis-related gene expression on photosystem ii during peg stress[J]. Acta Physiologiae Plantarum, 2014, 36(8): 2219-2227.