Effect of Oil-water Mixed Solvent on the Antibacterial Effect of Essential Oils in Essential Oil Preparations

doi:10.11924/j.issn.1000-6850.casb18110126

Abstract

Abstract:

To investigate the antibacterial effect of compound essential oil based on oil-water mixed solvent on settled bacteria in air, the antibacterial effect of the compound essential oil A and essential oil antibacterial preparations were studied, and the effect of oil-water mixed solvent on antibacterial effect of essential oil were determined. The inhibition effect of compound essential oil A was measured by plate sedimentation. Essential oil antibacterial preparations were made with the ratio of compound essential oil A and oil-water mixed solvent between 1:1 to 1:6, and five kinds of oil-water mixed solvents were chosen, such as PEG-40 hydrogenated castor oil, tween 80, dimethyl sulfoxide (DMSO), span 85 and double de-ethanol. Checkerboard dilution method was used to measure the inhibition effect of these preparations. And the results showed that, the bacteriostatic rate of compound essential oil A was larger than 80%, and the best bacteriostatic rate was 48.92% when the essential oil antibacterial preparation has exposed on flat for six hours. When PEG-40 in oil-water mixed solvent got the best solubilization effect, the ratio of essential oil, PEG-40 and double-de-ethanol was 1:2:2 when the concentration of compound essential oil was 1%, and was 1:3:3 when the concentration was 3%. PEG-40 showed no inhibition effect on settled bacteria in air, and the inhibition effect of double-de-ethanol and compound essential oil was additive. Based on the research above, when the plant essential oil is applied to industrial production, the type and proportion of the oil-water mixed solvent have certain actual effect on the essential oil product.

Key words: oil-water mixed solvent, compound essential oil, settling bacteria, essential oil antibacterial preparations, antibacterial effect

CLC Number:

TQ423

Ma Yiyi, Wang Wencui, Huang Haiyan, Yao Lei. Effect of Oil-water Mixed Solvent on the Antibacterial Effect of Essential Oils in Essential Oil Preparations[J]. Chinese Agricultural Science Bulletin, 2020, 36(7): 135-142.

Figures/Tables 14

References 26

[1]	姚雷 . 解读精油的功效性和安全性[J]. 中国化妆品, 2016(Z6):52-57.
[2]	秦臻, 徐军, 张立伟 . 连翘及连翘叶体外抑菌作用研究[J]. 食品工程, 2013(2):49-52.
[3]	吕飞 . 天然植物精油的抑菌活性及其作用机理研究[D]. 北京:北京化工大学, 2011.
[4]	吴慧清, 吴清平, 石立三 , 等. 植物精油对微生物的抑菌效果评估研究[J]. 食品科学, 2008,29(12):83-86.
[5]	de Groot A C, Schmidt E . Essential oils part I: introduction[J]. Dermatitis, 2016,27(2):39-42.
[6]	Su Jiaqi, Guo Qing, Mao Like , et al. Effect of gum arabic on the storage stability and antibacterial ability of beta-lactoglobulin stabilized D-limonene emulsion[J]. Food Hydrocolloids, 2018,84:75-83.
[7]	Alves Susana, Duarte Andreia, Sousa Sonia , et al. Study of the major essential oil compounds of coriandrum sativum against ccinetobacter baumannii and the effect of linalool on adhesion, biofilms and quorum sensing[J]. Biofouling, 2016,32(2):155-165.
[8]	符继红, 唐军, 廖享 , 等. 基于抑菌活性成分的薰衣草精油组效关系研究[J]. 质谱学报, 2015,36(5):403-410.
[9]	Pattnaik S, Subramanyam V R, Bapaji M , et al. Antibacterial and antifungal activity of aromatic constituents of essential oils[J]. Microbios, 1997,89:39-46.
[10]	潘岩, 白红彤, 李慧 , 等. 栽培地区、采收季节和株龄对迷迭香精油成分和抑菌活性的影响[J]. 植物学报, 2012,47(6):625-636.
[11]	Rachmawati Heni, Novel Miranti Anggraeni, Ayu Sri , et al. The in vitro-in vivo safety confirmation of PEG-40 hydrogenated castor oil as a surfactant for oral nanoemulsion formulation[J]. Scientia Pharmaceutica, 2017,85(2).
[12]	Pang Jinyu, Du Sujun, Chang Runtian , et al. Interfacial rheology of mixed surfactants at the oil/water interface[J]. Journal of Surfactants and Detergents, 2015,18(5):747-753.
[13]	Krishnamoorthy Anand Narayanan, Zeman Johannes, Holm Christian , et al. Preferential solvation and ion association properties in aqueous dimethyl sulfoxide solutions[J]. Physical Chemistry Chemical Physics, 2016,18(45):31312-31322
[14]	Owusu Apenten Richard K, Zhu Qing-Hong . Interfacial parameters for selected Spans and Tweens at the hydrocarbon-water interface[J]. Food Hydrocolloids, 1996,10(1):27-30.
[15]	孙延芳, 梁宗锁, 刘新桐 , 等. 一种新型白蒿精油空气清新剂的研制[J]. 应用化工, 2011,40(11):1861-1863.
[16]	Yousef R T, Khawam M N, Tawashi R , et al. The effect of solubilizers on the bacteriostatic action of sulfanilamide.[J]. Arzneimittel-Forschung, 1966,16(4).
[17]	Kinoshita M, Hayashi T . Entropic enrichment of cosolvent near a very large solute immersed in solvent-cosolvent binary mixture: Anomalous dependence on bulk cosolvent concentration[J]. Journal of Molecular Liquids, 2017,247:403-410.
[18]	Budkov Y A, Kolesnikov A L, Georgi N , et al. A statistical theory of cosolvent-induced coil-globule transitions in dilute polymer solution[J]. Journal of Chemical Physics, 2014,141(1):148.
[19]	Davidson P M, Parish M E . Methods for testing the efficacy of food antimicrobials[J]. Food Technology, 1989,43:148-155.
[20]	李忠琴, 关瑞章, 汪黎虹 , 等. 六种中药及其复方对鳗鲡致病性气单胞菌的体外抑制作用[J]. 水生生物学报, 2012,36(1):85-92.
[21]	宋繁华 . 清爽柠檬洗手液[P]. 中国专利, 103565677A, 2014.
[22]	Hilbig J, Ma Q M, Davidson P M , et al. Physical and antimicrobial properties of cinnamon bark oil co-nanoemulsified by lauric arginate and Tween 80[J]. International Journal of Food Microbiology, 2016,233:52-59.
[23]	Ma Q M, Davidson P M, Zhong Q X . Antimicrobial properties of microemulsions formulated with essential oils, soybean oil, and Tween 80[J]. International Journal of Food Microbiology, 2016,226:20-25.
[24]	孙存玉 . 天然植物精油抗菌剂的开发及应用[D]. 镇江:江苏大学, 2015.
[25]	陈婷婷 . 天然植物型抑菌剂的配制及抗菌性能评价[J]. 科技风, 2017(23):177.
[26]	丁华, 王建清, 王玉峰 , 等. 罗勒精油和Nisin复配抑菌剂抑菌效果的研究[J]. 中国调味品, 2016,41(7):20-25.

序号	菌种拉丁名	菌种中文名	采集点
1	Bacillus amyloliquefaciens	解淀粉芽胞杆菌	卫生间
2	Bacillus megaterium	巨大芽孢杆菌	卫生间
3	Bacillus subblls	/	卫生间、走廊、宿舍
4	Bacillus thuringiensis	苏云金芽孢杆菌	卫生间、办公室、走廊
5	Bacillus cereus	蜡样芽胞杆菌	卫生间、办公室、走廊、宿舍
6	Pseudomonas hibiscicola	栖木槿假单胞菌	办公室、宿舍
7	Stenotrophomonasmaltophilia	嗜麦芽寡养单胞菌	走廊
8	Bacillus licheniformis	地衣芽胞杆菌	走廊
9	Bacillus altitudinis	高地芽孢杆菌	办公室、走廊
10	Aeromonas media	中间气单胞菌	走廊
11	Staphylococcus saprophyticus	腐生性葡萄球菌	宿舍
12	Bacillus velezensis	贝莱斯芽胞杆菌	宿舍

序号	菌种拉丁名	菌种中文名	采集点
1	Bacillus amyloliquefaciens	解淀粉芽胞杆菌	卫生间
2	Bacillus megaterium	巨大芽孢杆菌	卫生间
3	Bacillus subblls	/	卫生间、走廊、宿舍
4	Bacillus thuringiensis	苏云金芽孢杆菌	卫生间、办公室、走廊
5	Bacillus cereus	蜡样芽胞杆菌	卫生间、办公室、走廊、宿舍
6	Pseudomonas hibiscicola	栖木槿假单胞菌	办公室、宿舍
7	Stenotrophomonasmaltophilia	嗜麦芽寡养单胞菌	走廊
8	Bacillus licheniformis	地衣芽胞杆菌	走廊
9	Bacillus altitudinis	高地芽孢杆菌	办公室、走廊
10	Aeromonas media	中间气单胞菌	走廊
11	Staphylococcus saprophyticus	腐生性葡萄球菌	宿舍
12	Bacillus velezensis	贝莱斯芽胞杆菌	宿舍

成分	含量
酪蛋白胰酶消化物/g	15
大豆粉木瓜蛋白酶消化物/g	5
氯化钠/g	5
琼脂/g	15
纯化水/mL	1000

成分	含量
酪蛋白胰酶消化物/g	15
大豆粉木瓜蛋白酶消化物/g	5
氯化钠/g	5
琼脂/g	15
纯化水/mL	1000

序号	英文名	中文名	含量/%	序号	英文名	中文名	含量/%
1	limonene	柠檬烯	36.17	19	spathulenol	桉油烯醇	0.29
2	linalool	芳樟醇	27.99	20	linalool oxide B	芳樟醇氧化物B	0.26
3	menthol	薄荷醇	8.62	21	camphene	莰烯	0.23
4	thujone	侧柏酮	7.44	22	menthyl acetate	乙酸薄荷酯	0.21
5	citral	柠檬醛	4.34	23	safrole	黄樟素	0.20
6	beta-citral	β-柠檬醛	2.73	24	gamma-cadinene	γ-荜澄茄烯	0.19
7	menthone	薄荷酮	1.05	25	humulene	α-律草烯	0.19
8	beta-caryophyllene	反式石竹烯	0.91	26	terpinen-4-ol	4-萜品醇	0.18
9	myrcene	月桂烯	0.78	27	p-cymene	对伞花烃	0.18
10	eucalyptol	桉叶油醇	0.73	28	6-methyl-5-hepten-2-one	甲基庚烯酮	0.17
11	alpha-pinene	α-蒎烯	0.55	29	piperitone	胡椒酮	0.16
12	sabenene	桧烯	0.49	30	beta-pinene	β-蒎烯	0.15
13	geraniol	香叶醇	0.42	31	alpha-terpineol	α-萜品醇	0.14
14	isomenthone	异薄荷酮	0.33	32	camphor	樟脑	0.13
15	caryophyllene oxide	石竹素	0.33	33	beta-phellandrene	β-水芹烯	0.12
16	geranyl acetate	乙酸香叶酯	0.32	34	cis-4-thujanol	顺式-4-侧柏醇	0.12
17	linalool oxide A	芳樟醇氧化物A	0.31	35	dihydrolinalool	二氢芳樟醇	0.12
18	neointermedeol	新臭根醇	0.29