Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (7): 135-142.doi: 10.11924/j.issn.1000-6850.casb18110126
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Ma Yiyi1, Wang Wencui1, Huang Haiyan2, Yao Lei1()
Received:
2018-11-29
Revised:
2019-02-28
Online:
2020-03-05
Published:
2020-03-02
Contact:
Lei Yao
E-mail:yaolei@sjtu.edu.cn
CLC Number:
Ma Yiyi, Wang Wencui, Huang Haiyan, Yao Lei. Effect of Oil-water Mixed Solvent on the Antibacterial Effect of Essential Oils in Essential Oil Preparations[J]. Chinese Agricultural Science Bulletin, 2020, 36(7): 135-142.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb18110126
序号 | 菌种拉丁名 | 菌种中文名 | 采集点 |
---|---|---|---|
1 | Bacillus amyloliquefaciens | 解淀粉芽胞杆菌 | 卫生间 |
2 | Bacillus megaterium | 巨大芽孢杆菌 | 卫生间 |
3 | Bacillus subblls | / | 卫生间、走廊、宿舍 |
4 | Bacillus thuringiensis | 苏云金芽孢杆菌 | 卫生间、办公室、走廊 |
5 | Bacillus cereus | 蜡样芽胞杆菌 | 卫生间、办公室、走廊、宿舍 |
6 | Pseudomonas hibiscicola | 栖木槿假单胞菌 | 办公室、宿舍 |
7 | Stenotrophomonasmaltophilia | 嗜麦芽寡养单胞菌 | 走廊 |
8 | Bacillus licheniformis | 地衣芽胞杆菌 | 走廊 |
9 | Bacillus altitudinis | 高地芽孢杆菌 | 办公室、走廊 |
10 | Aeromonas media | 中间气单胞菌 | 走廊 |
11 | Staphylococcus saprophyticus | 腐生性葡萄球菌 | 宿舍 |
12 | Bacillus velezensis | 贝莱斯芽胞杆菌 | 宿舍 |
序号 | 菌种拉丁名 | 菌种中文名 | 采集点 |
---|---|---|---|
1 | Bacillus amyloliquefaciens | 解淀粉芽胞杆菌 | 卫生间 |
2 | Bacillus megaterium | 巨大芽孢杆菌 | 卫生间 |
3 | Bacillus subblls | / | 卫生间、走廊、宿舍 |
4 | Bacillus thuringiensis | 苏云金芽孢杆菌 | 卫生间、办公室、走廊 |
5 | Bacillus cereus | 蜡样芽胞杆菌 | 卫生间、办公室、走廊、宿舍 |
6 | Pseudomonas hibiscicola | 栖木槿假单胞菌 | 办公室、宿舍 |
7 | Stenotrophomonasmaltophilia | 嗜麦芽寡养单胞菌 | 走廊 |
8 | Bacillus licheniformis | 地衣芽胞杆菌 | 走廊 |
9 | Bacillus altitudinis | 高地芽孢杆菌 | 办公室、走廊 |
10 | Aeromonas media | 中间气单胞菌 | 走廊 |
11 | Staphylococcus saprophyticus | 腐生性葡萄球菌 | 宿舍 |
12 | Bacillus velezensis | 贝莱斯芽胞杆菌 | 宿舍 |
序号 | 英文名 | 中文名 | 含量/% | 序号 | 英文名 | 中文名 | 含量/% |
---|---|---|---|---|---|---|---|
1 | limonene | 柠檬烯 | 36.17 | 19 | spathulenol | 桉油烯醇 | 0.29 |
2 | linalool | 芳樟醇 | 27.99 | 20 | linalool oxide B | 芳樟醇氧化物B | 0.26 |
3 | menthol | 薄荷醇 | 8.62 | 21 | camphene | 莰烯 | 0.23 |
4 | thujone | 侧柏酮 | 7.44 | 22 | menthyl acetate | 乙酸薄荷酯 | 0.21 |
5 | citral | 柠檬醛 | 4.34 | 23 | safrole | 黄樟素 | 0.20 |
6 | beta-citral | β-柠檬醛 | 2.73 | 24 | gamma-cadinene | γ-荜澄茄烯 | 0.19 |
7 | menthone | 薄荷酮 | 1.05 | 25 | humulene | α-律草烯 | 0.19 |
8 | beta-caryophyllene | 反式石竹烯 | 0.91 | 26 | terpinen-4-ol | 4-萜品醇 | 0.18 |
9 | myrcene | 月桂烯 | 0.78 | 27 | p-cymene | 对伞花烃 | 0.18 |
10 | eucalyptol | 桉叶油醇 | 0.73 | 28 | 6-methyl-5-hepten-2-one | 甲基庚烯酮 | 0.17 |
11 | alpha-pinene | α-蒎烯 | 0.55 | 29 | piperitone | 胡椒酮 | 0.16 |
12 | sabenene | 桧烯 | 0.49 | 30 | beta-pinene | β-蒎烯 | 0.15 |
13 | geraniol | 香叶醇 | 0.42 | 31 | alpha-terpineol | α-萜品醇 | 0.14 |
14 | isomenthone | 异薄荷酮 | 0.33 | 32 | camphor | 樟脑 | 0.13 |
15 | caryophyllene oxide | 石竹素 | 0.33 | 33 | beta-phellandrene | β-水芹烯 | 0.12 |
16 | geranyl acetate | 乙酸香叶酯 | 0.32 | 34 | cis-4-thujanol | 顺式-4-侧柏醇 | 0.12 |
17 | linalool oxide A | 芳樟醇氧化物A | 0.31 | 35 | dihydrolinalool | 二氢芳樟醇 | 0.12 |
18 | neointermedeol | 新臭根醇 | 0.29 |
序号 | 英文名 | 中文名 | 含量/% | 序号 | 英文名 | 中文名 | 含量/% |
---|---|---|---|---|---|---|---|
1 | limonene | 柠檬烯 | 36.17 | 19 | spathulenol | 桉油烯醇 | 0.29 |
2 | linalool | 芳樟醇 | 27.99 | 20 | linalool oxide B | 芳樟醇氧化物B | 0.26 |
3 | menthol | 薄荷醇 | 8.62 | 21 | camphene | 莰烯 | 0.23 |
4 | thujone | 侧柏酮 | 7.44 | 22 | menthyl acetate | 乙酸薄荷酯 | 0.21 |
5 | citral | 柠檬醛 | 4.34 | 23 | safrole | 黄樟素 | 0.20 |
6 | beta-citral | β-柠檬醛 | 2.73 | 24 | gamma-cadinene | γ-荜澄茄烯 | 0.19 |
7 | menthone | 薄荷酮 | 1.05 | 25 | humulene | α-律草烯 | 0.19 |
8 | beta-caryophyllene | 反式石竹烯 | 0.91 | 26 | terpinen-4-ol | 4-萜品醇 | 0.18 |
9 | myrcene | 月桂烯 | 0.78 | 27 | p-cymene | 对伞花烃 | 0.18 |
10 | eucalyptol | 桉叶油醇 | 0.73 | 28 | 6-methyl-5-hepten-2-one | 甲基庚烯酮 | 0.17 |
11 | alpha-pinene | α-蒎烯 | 0.55 | 29 | piperitone | 胡椒酮 | 0.16 |
12 | sabenene | 桧烯 | 0.49 | 30 | beta-pinene | β-蒎烯 | 0.15 |
13 | geraniol | 香叶醇 | 0.42 | 31 | alpha-terpineol | α-萜品醇 | 0.14 |
14 | isomenthone | 异薄荷酮 | 0.33 | 32 | camphor | 樟脑 | 0.13 |
15 | caryophyllene oxide | 石竹素 | 0.33 | 33 | beta-phellandrene | β-水芹烯 | 0.12 |
16 | geranyl acetate | 乙酸香叶酯 | 0.32 | 34 | cis-4-thujanol | 顺式-4-侧柏醇 | 0.12 |
17 | linalool oxide A | 芳樟醇氧化物A | 0.31 | 35 | dihydrolinalool | 二氢芳樟醇 | 0.12 |
18 | neointermedeol | 新臭根醇 | 0.29 |
增溶度 | 溶液现象 |
---|---|
0.0 | 混合物分层,水层浑浊,水中有絮状物 |
0.1 | 混合物呈乳白色,且分层 |
0.2 | 混合物呈半透明状,且分层 |
0.3 | 混合物浑浊,且表面或底部有少量精油液滴 |
0.4 | 混合物呈乳白色,且表面或底部有少量精油液滴 |
0.5 | 混合物呈乳白色 |
0.6 | 混合物呈半透明状,且表面或底部有少量精油液滴或白色絮状物 |
0.7 | 溶液澄清透明,但上层或底部有少量精油液滴 |
0.8 | 溶液呈半透明状 |
0.9 | 溶液澄清透明,但上层有少量白色絮状物 |
1.0 | 溶液完全澄清、透明 |
增溶度 | 溶液现象 |
---|---|
0.0 | 混合物分层,水层浑浊,水中有絮状物 |
0.1 | 混合物呈乳白色,且分层 |
0.2 | 混合物呈半透明状,且分层 |
0.3 | 混合物浑浊,且表面或底部有少量精油液滴 |
0.4 | 混合物呈乳白色,且表面或底部有少量精油液滴 |
0.5 | 混合物呈乳白色 |
0.6 | 混合物呈半透明状,且表面或底部有少量精油液滴或白色絮状物 |
0.7 | 溶液澄清透明,但上层或底部有少量精油液滴 |
0.8 | 溶液呈半透明状 |
0.9 | 溶液澄清透明,但上层有少量白色絮状物 |
1.0 | 溶液完全澄清、透明 |
增溶剂 | 复方精油:增溶剂:双脱乙醇 | 25℃静置1周 增溶度 | 50℃静置1周 增溶度 | 增溶剂 | 复方精油:增溶剂:双脱乙醇 | 25℃静置1周 增溶度 | 50℃静置1周 增溶度 |
---|---|---|---|---|---|---|---|
1% PEG-40 | 1:1:1 | 0.90±0.00 | 0.93±0.06 | 3%吐温80 | 1:1:1 | 0.50±0.00 | 0.50±0.00 |
1:2:2 | 0.97±0.06 | 1.00±0.00 | 1:2:2 | 0.60±0.00 | 0.60±0.00 | ||
1:3:3 | 1.00±0.00 | 1.00±0.00 | 1:3:3 | 0.70±0.00 | 0.90±0.01 | ||
1:4:4 | 1.00±0.00 | 1.00±0.00 | 1:4:4 | 0.93±0.06 | 1.00±0.00 | ||
1:5:5 | 1.00±0.00 | 1.00±0.00 | 1:5:5 | 1.00±0.00 | 1.00±0.00 | ||
1:6:6 | 1.00±0.00 | 1.00±0.00 | 1:6:6 | 1.00±0.00 | 1.00±0.00 | ||
3% PEG-40 | 1:1:1 | 0.80±0.00 | 0.80±0.00 | 1%DMSO | 1:1:1 | 0.67±0.06 | 0.70±0.00 |
1:2:2 | 0.90±0.00 | 0.97±0.06 | 1:2:2 | 0.70±0.00 | 0.73±0.06 | ||
1:3:3 | 0.97±0.06 | 1.00±0.00 | 1:3:3 | 0.87±0.06 | 0.90±0.00 | ||
1:4:4 | 1.00±0.00 | 1.00±0.00 | 1:4:4 | 0.90±0.00 | 0.90±0.00 | ||
1:5:5 | 1.00±0.00 | 1.00±0.00 | 1:5:5 | 0.90±0.00 | 1.00±0.00 | ||
1:6:6 | 1.00±0.00 | 1.00±0.00 | 1:6:6 | 0.90±0.00 | 1.00±0.00 | ||
1%吐温80 | 1:1:1 | 0.50±0.00 | 0.80±0.00 | 3%DMSO | 1:1:1 | 0.60±0.00 | 0.60±0.00 |
1:2:2 | 0.77±0.06 | 0.97±0.06 | 1:2:2 | 0.60±0.00 | 0.67±0.06 | ||
1:3:3 | 0.97±0.06 | 1.00±0.00 | 1:3:3 | 0.70±0.00 | 0.70±0.00 | ||
1:4:4 | 1.00±0.00 | 1.00±0.00 | 1:4:4 | 0.70±0.00 | 0.70±0.00 | ||
1:5:5 | 1.00±0.00 | 1.00±0.00 | 1:5:5 | 0.70±0.00 | 0.70±0.00 | ||
1:6:6 | 1.00±0.00 | 1.00±0.00 | 1:6:6 | 0.73±0.06 | 0.73±0.06 |
增溶剂 | 复方精油:增溶剂:双脱乙醇 | 25℃静置1周 增溶度 | 50℃静置1周 增溶度 | 增溶剂 | 复方精油:增溶剂:双脱乙醇 | 25℃静置1周 增溶度 | 50℃静置1周 增溶度 |
---|---|---|---|---|---|---|---|
1% PEG-40 | 1:1:1 | 0.90±0.00 | 0.93±0.06 | 3%吐温80 | 1:1:1 | 0.50±0.00 | 0.50±0.00 |
1:2:2 | 0.97±0.06 | 1.00±0.00 | 1:2:2 | 0.60±0.00 | 0.60±0.00 | ||
1:3:3 | 1.00±0.00 | 1.00±0.00 | 1:3:3 | 0.70±0.00 | 0.90±0.01 | ||
1:4:4 | 1.00±0.00 | 1.00±0.00 | 1:4:4 | 0.93±0.06 | 1.00±0.00 | ||
1:5:5 | 1.00±0.00 | 1.00±0.00 | 1:5:5 | 1.00±0.00 | 1.00±0.00 | ||
1:6:6 | 1.00±0.00 | 1.00±0.00 | 1:6:6 | 1.00±0.00 | 1.00±0.00 | ||
3% PEG-40 | 1:1:1 | 0.80±0.00 | 0.80±0.00 | 1%DMSO | 1:1:1 | 0.67±0.06 | 0.70±0.00 |
1:2:2 | 0.90±0.00 | 0.97±0.06 | 1:2:2 | 0.70±0.00 | 0.73±0.06 | ||
1:3:3 | 0.97±0.06 | 1.00±0.00 | 1:3:3 | 0.87±0.06 | 0.90±0.00 | ||
1:4:4 | 1.00±0.00 | 1.00±0.00 | 1:4:4 | 0.90±0.00 | 0.90±0.00 | ||
1:5:5 | 1.00±0.00 | 1.00±0.00 | 1:5:5 | 0.90±0.00 | 1.00±0.00 | ||
1:6:6 | 1.00±0.00 | 1.00±0.00 | 1:6:6 | 0.90±0.00 | 1.00±0.00 | ||
1%吐温80 | 1:1:1 | 0.50±0.00 | 0.80±0.00 | 3%DMSO | 1:1:1 | 0.60±0.00 | 0.60±0.00 |
1:2:2 | 0.77±0.06 | 0.97±0.06 | 1:2:2 | 0.60±0.00 | 0.67±0.06 | ||
1:3:3 | 0.97±0.06 | 1.00±0.00 | 1:3:3 | 0.70±0.00 | 0.70±0.00 | ||
1:4:4 | 1.00±0.00 | 1.00±0.00 | 1:4:4 | 0.70±0.00 | 0.70±0.00 | ||
1:5:5 | 1.00±0.00 | 1.00±0.00 | 1:5:5 | 0.70±0.00 | 0.70±0.00 | ||
1:6:6 | 1.00±0.00 | 1.00±0.00 | 1:6:6 | 0.73±0.06 | 0.73±0.06 |
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