Genetic Analysis and Gene Mapping for Melon Rind Color

doi:10.11924/j.issn.1000-6850.casb18110127

Abstract

Abstract: To explore the inheritance and genetic loci of melon rind color, the yellow-rind line B432 and green-rind lines B168 and B421 were used as parent to construct six generations of genetic population (P1, P2, F1, F2, BC1P1, BC1P2) to analyze the inheritance of melon rind color. In addition, the BSA-seq and whole genome resequencing technology were used to locate the genetic loci responsible for melon rind color. The results show that the melon rind color is controlled by two genes, of which green has a dominant epistatic effect on white and white is dominant to yellow. The rind color related genes are mapped to the 0.02-5.7 Mb and 0.08- 9.5 Mb intervals of chromosome 4 and chromosome 10, respectively. This experiment preliminarily elucidates the genetic mechanism and the location of the genes controlling the rind color of melon, and could provide a basis for the subsequent fine-grained gene mapping and molecular marker-assisted selection of melon rind color.

References

[1] Chayut N, Yuan H, Ohali S, et al. A bulk segregant transcriptome analysis reveals metabolic and cellular processes associated with orange allelic variation and fruit β-carotene accumulation in melon fruit[J]. BMC Plant Biology,2015,15(1):274.
[2] Freilich S, Lev S, Gonda I, et al. Systems approach for exploring the intricate associations between sweetness, color and aroma in melon fruits[J]. BMC Plant Biology,15,1(2015-03-03),2015,15(1):71.
[3] 李德泽.浅谈薄皮甜瓜育种[J].北方园艺,2003(2):4-5.
[4] Cuevas H E, Staub J E, Simon P W, et al. A consensus linkage map identifies genomic regions controlling fruit maturity and beta-carotene-associated flesh color in melon ( Cucumis melo, L.)[J]. Theoretical and Applied Genetics,2009,119(4):741-756.
[5] Cuevas H E, Staub J E, Simon P W. Inheritance of beta-carotene-associated mesocarp color and fruit maturity of melon ( Cucumis melo, L.)[J]. Euphytica,2010,173(1):129-140.
[6] 杨光华,范荣,杨小锋,等.甜瓜果实颜色3个质量性状基因的定位[J].园艺学报,2014,41(5):898-906.
[7] Feder A, Burger J, Gao S, et al. Focus on Metabolism: A kelch domain-containing F-Box coding gene negatively regulates flavonoid accumulation in muskmelon[J]. Plant Physiology,2015,169(3):1714.
[8] Gur A, Tzuri G, Meir A, et al. Genome-wide linkage-disequilibrium mapping to the candidate gene level in melon ( Cucumis melo )[J]. Scientific Reports,2017,7(1):9770.
[9] Garcia-Mas J, Benjak A, Sanseverino W, et al. The genome of melon (Cucumis melo L.)[J]. Proceedings of the National Academy of Sciences,2012,109(29):11872
[10] Takagi H, Abe A, Yoshida K, et al. QTL-seq: rapid mapping of quantitative trait loci in rice by whole genome resequencing of DNA from two bulked populations[J]. Plant Journal,2013,74(1):174-183.
[11] Sun J, Yang L, Wang J, et al. Identification of a cold-tolerant locus in rice ( Oryza sativa, L.) using bulked segregant analysis with a next-generation sequencing strategy[J]. Rice,2018,11(1):24.
[12] Li P, Du C, Zhang Y, et al. Combined bulked segregant sequencing and traditional linkage analysis for identification of candidate gene for purple leaf sheath in maize[J]. Plos One,2018,13(1):e0190670.
[13] 张尧锋,张冬青,余华胜,等.基于极端混合池(BSA)全基因组重测序的甘蓝型油菜有限花序基因定位[J].中国农业科学,2018,51(16): 3029-3039.
[14] Chagué V, Mercier J C, Guénard M, et al. Identification of RAPD markers linked to a locus involved in quantitative resistance, to TYLCV in tomato by bulked segregant analysis[J]. Theoretical and Applied Genetics,1997,95(4):671-677.
[15] Dong W, Wu D, Li G, et al. Next-generation sequencing from bulked segregant analysis identifies a dwarfism gene in watermelon[J]. Scientific Reports,2018,8(1):2908.
[16] Meng R, Zhang J, An L, et al. Expression profiling of several gene families involved in anthocyanin biosynthesis in apple ( Malus domestica, Borkh.) skin during fruit development[J]. Journal of Plant Growth Regulation,2016,35(2):1-16.
[17] Moriya S, Kunihisa M, Okada K, et al. Allelic composition of MdMYB1, drives red skin color intensity in apple ( Malus × domestica, Borkh.) and its application to breeding[J]. Euphytica,2017,213(4):78.
[18] Ballester A R, Molthoff J, De R V, et al. Biochemical and molecular analysis of pink tomatoes: deregulated expression of the gene encoding transcription factor SlMYB12 leads to pink tomato fruit color[J]. Plant Physiology,2010,152(1):71-84.
[19] Jung H J, Veerappan K, Hwang I, et al. New SNPs and InDel Variations in SlMYB12, associated with regulation of pink color in tomato[J]. Tropical Plant Biology,2017:1-8.
[20] Liu H Q, Jiao J Q, Liang X J, et al. Map-based cloning, identifcation and characterization of the w gene controlling white immature fruit color in cucumber (Cucumis sativus L.)[J]. Theoretical and Applied Genetics,2016,129:1247–1256
[21] 段颖,向成钢,刘新艳,等.印度南瓜果皮结构与色素组成对果皮颜色的影响[C].中国园艺学会南瓜研究分会2017年学术年会论文集,2017.
[22] 白立华,刘艳,孙喜梅,等.厚皮甜瓜果实主要性状遗传规律的初步研究[C].北方遗传资源的保护与利用研讨会论文汇编,2010.
[23] Monforte A J, Oliver M, Gonzalo M J, et al. Identification of quantitative trait loci involved in fruit quality traits in melon ( Cucumis melo, L.)[J]. Theoretical and Applied Genetics,2004,108(4):750-758.