Alkali Extraction Process of Chondroitin Sulfate in Goose Bone: Optimization

doi:10.11924/j.issn.1000-6850.casb19030044

Abstract

Abstract:

To strengthen the development and utilization of goose by-products, improve their added value, and reduce the pressure of untreated by-products on the environment, this study took the bones of Mayang goose as the raw material, and optimized the alkali extraction process of chondroitin sulfate (CS). Single-factor and orthogonal experiments were carried out from four different factors, namely, alkali extraction concentration, solid-liquid ratio, ultrasonic time and ultrasonic temperature. Phloroglucinol spectrophotometric method was used to determine the concentration of CS in the extraction solution, so as to obtain the optimal condition for alkali extraction. The results showed that the extraction rate of chondroitin sulfate reached the maximum of 17.66% when the alkali concentration was 5%, the alkali extraction time was 4 h, the solid-liquid ratio was 1:6, and the alkali extraction temperature was 30℃. The ultrasonic assisted alkali extraction process for extracting CS from goose bone established in this study can improve the production rate and reduce the usage of alkali compared with the existing domestic production methods, so as to reduce the production costs and environmental pollution.

Key words: goose bone, chondroitin sulfate (CS), ultrasonic, alkali extraction

CLC Number:

TS251.94

Xie Jing, Zhou Yanhong, Yang Yucheng, Yin Yijuan, Jin Chenzhong, Lu Chao. Alkali Extraction Process of Chondroitin Sulfate in Goose Bone: Optimization[J]. Chinese Agricultural Science Bulletin, 2020, 36(18): 141-146.

Figures/Tables 7

References 25

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水平	因素
水平	A碱液浓度/%	B碱提时间/h	C料液比	D碱提温度/℃
1	4	3	1:4	20
2	5	4	1:6	30
3	6	5	1:8	40

水平	因素
水平	A碱液浓度/%	B碱提时间/h	C料液比	D碱提温度/℃
1	4	3	1:4	20
2	5	4	1:6	30
3	6	5	1:8	40

试验号	A碱液浓度	B碱提时间	C料液比	D碱提温度	提取率/%
1	1(4%)	1(3h)	1(1:4)	1(20℃)	14.35
2	1	2(4h)	2(1:6)	2(30℃)	16.43
3	1	3(5h)	3(1:8)	3(40℃)	13.66
4	2(5%)	1	2	3	15.39
5	2	2	3	1	16.31
6	2	3	1	2	15.98
7	3(6%)	1	3	2	11.22
8	3	2	1	3	13.74
9	3	3	2	1	11.12
K₁	44.44	40.96	42.07	41.78
K₂	47.68	46.48	42.94	43.63
K₃	36.08	40.76	41.19	39.79
K₁	14.81	13.65	14.31	13.92
K₂	15.89	15.49	15.31	14.54
K₃	12.02	13.58	13.73	13.21
R	3.87	1.91	1.58	1.33
主次顺序		A>B>C>D
最优方案		A₂B₂C₂D₂

试验号	A碱液浓度	B碱提时间	C料液比	D碱提温度	提取率/%
1	1(4%)	1(3h)	1(1:4)	1(20℃)	14.35
2	1	2(4h)	2(1:6)	2(30℃)	16.43
3	1	3(5h)	3(1:8)	3(40℃)	13.66
4	2(5%)	1	2	3	15.39
5	2	2	3	1	16.31
6	2	3	1	2	15.98
7	3(6%)	1	3	2	11.22
8	3	2	1	3	13.74
9	3	3	2	1	11.12
K₁	44.44	40.96	42.07	41.78
K₂	47.68	46.48	42.94	43.63
K₃	36.08	40.76	41.19	39.79
K₁	14.81	13.65	14.31	13.92
K₂	15.89	15.49	15.31	14.54
K₃	12.02	13.58	13.73	13.21
R	3.87	1.91	1.58	1.33
主次顺序		A>B>C>D
最优方案		A₂B₂C₂D₂