Abstract:

This study was conducted to evaluate the effect of soil disinfection with dazomet (DA) on bacterial communities in the soil with cabbage clubroot disease at the high mountain area, aiming to control cabbage clubroot disease by soil disinfection. High throughput sequencing technology of bacterial 16S ribosomal genes was utilized to study the effect of dazomet (DA) soil disinfection on soil bacterial community structures of cabbage clubroot disease at the high mountain area. High throughput sequencing gained 9445 bacterial OTUs (operational taxonomic units), of which 2327 bacterial OTUs (24.64%) were commonly detected in three treatments. The community structures of bacteria at phylum and genus level in the DA-treated soil were similar to those in CK, but a part of phyla and genera had greater changes in abundance. All four types of bacterial α diversity indexes in the DA-treated soil had no significant difference with CK (P>0.05). Analysis of β diversity index showed that the distance in the DA treatment was closer to the fluazinam treatment, but was farther to CK. Metastats analysis demonstrated that, compared to CK, there were 1 phylum and 24 genera of bacteria with significant changes in abundance in the DA treatment, of which soil beneficial bacteria were significantly increased, whereas there were 4 phyla and 16 genera of bacteria that were detected to have significant changes in abundance in the fluazinam treatment. The above results showed that soil disinfection with DA had no significant effect on bacterial diversities, and less influence on bacterial community structures than fluazinam, and was benefit to the recovery and re-construction of soil bacterial community structures.

Key words: soil disinfection, dazomet, cabbage, clubroot disease, microbiome, microbial diversity