Cloning and Functional Analysis of Wheat TaPLD-2 Gene

doi:10.11924/j.issn.1000-6850.casb20190500215

Abstract

Abstract:

The study aims to find new salt-tolerant genes and breed new salt-tolerant varieties. 23 wheat varieties were used as materials to clone and sequence polymorphisms of TaPLD-2 gene, and TaPLD-2 gene expression patterns in response to salt stress was studied. The results showed that the full-length coding frame of TaPLD-2 gene was 1301 bp, located on the 2DS chromosome, and the protein was a hydrophilic protein and located in cytoplasm. The protein had no transmembrane region and belonged to the soluble NAD (P) (H) oxidoreductase family. The TaPLD-2 gene was induced to up-regulated expression by salt stress, and the expression level was 1.8 times that of the control group at 24 h after salt stress, and reached the highest at 48 h, which was 5 times that of the control group. The analysis result showed that the SNP loci had some relevance to relative root length, relative plant height, and K⁺ and Na⁺ contents of salt-treated plants.

Key words: wheat, gene polymorphism, transcriptome analysis, salt stress, bioinformatics analysis

CLC Number:

Song Shaoshuai, Wu Fan, Liu Huiwen, He Xiaoyan, Mu Ping. Cloning and Functional Analysis of Wheat TaPLD-2 Gene[J]. Chinese Agricultural Science Bulletin, 2020, 36(21): 98-103.

Figures/Tables 8

References 28

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引物名称	引物序列	TM值
F2DS	GGTGACCAGCATTACCGTCA	60.04
R2DS	AGATCGAACGAGGCGAAGAC	59.90
F βactin	TGCTATCCTTCGTTTGGACCTT	56.00
R βactin	AGCGGTTGTTGTGAGGGAGT	59.40

引物名称	引物序列	TM值
F2DS	GGTGACCAGCATTACCGTCA	60.04
R2DS	AGATCGAACGAGGCGAAGAC	59.90
F βactin	TGCTATCCTTCGTTTGGACCTT	56.00
R βactin	AGCGGTTGTTGTGAGGGAGT	59.40

引物名称	引物序列(5'to3')	退火温度/℃
2DF1	ATGTGGAGGGAGTGCAATTGGATGGGGTTC	65.1
2DR1	AGATAGGCACACACAGACACACACACCATG	63.7

引物名称	引物序列(5'to3')	退火温度/℃
2DF1	ATGTGGAGGGAGTGCAATTGGATGGGGTTC	65.1
2DR1	AGATAGGCACACACAGACACACACACCATG	63.7

多态性位点	处理时间/d	根长	株高	干重	Na⁺含量	k⁺含量
TaPLD-2-SNP1	2	-0.03	0.18	-0.02	0.25	0.27
	3	0.33^*	0.33^*	0.29	-0.87^*	0.56^*
	5	0.47^*	0.36^*	0.10	-0.79^*	0.48^*