The Response of Energy Beet BvGST Gene to Cadmium Stress in Escherichia coli

doi:10.11924/j.issn.1000-6850.casb20190600329

Abstract

Abstract: To further study the function of BvGST gene (LOC104898671) during the heavy metal cadmium tolerance in energy beet (Beta vulgaris L.), and to clarify its role and responsive characteristic in detoxification of cadmium ions in cells, 780016B/12You was chosen as the plant material in this study. BvGST gene was cloned and constructed into E. coli expression vector pEASY-Blunt E1 by RT-PCR, and transformed into E. coli BL21. SDS-PAGE showed that, there was an about 25.9 kDa band of his tag-BvGST fusion protein in recombinant bacteria total protein. When applied 0.5 mmol/L CdCl2, the recombinant strain expressing BvGST protein exhibited better growth than the control; meanwhile, GST activity in E. coli over-expressing BvGST was also much higher than that in control. It indicated that BvGST gene could detoxify cellular oxidative damage caused by cadmium stress, and improve Cd tolerance of E. coli by over-expressing glutathione transferase with high activity. The results suggest that BvGST plays an important role in the molecular mechanism of cadmium stress in energy beet.

CLC Number:

Q784

References

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