Effect of Bt Early Japonica Rice on Soil Microorganism Quantity and Transfer of Exogenous Genes of Rice

doi:10.11924/j.issn.1000-6850.casb20191000720

Abstract

Abstract:

To study the effect of transplanting Bt early japonica rice on soil microorganism number and the horizontal transfer of exogenous DNA of rice to soil microorganism, using field soil at different growth stages of rice as material, the number of soil microorganisms and exogenous DNA fragments such as Bt gene were measured with plate colony counting and PCR detection. The results showed that there were no significant differences among Bt early rice lines (‘HD1’, ‘HD2’) and corresponding recipient variety or among lines (‘HD3’, ‘HD4’) and their receptor in the number of bacteria, actinomycetes and fungus of rhizosphere and inter-row soil at rice growth and development stages of returning green, tillering, booting, heading and maturity. It was not found that exogenous DNA fragments of Bt early japonica rice lines were horizontally transferred to bacteria, actinomycetes and fungi of soil microorganism. There was no significant difference in the number of soil microorganisms between Bt early japonica rice and normal rice, and no exogenous DNA of Bt rice was found in soil microorganisms.

Key words: Bt early japonica rice, horizontal gene transfer, PCR, soil microorganism, environmental safety

CLC Number:

S511.22
Q786

Rongtian Li, Shitong Gao, Yi Gao, Changhua Liu. Effect of Bt Early Japonica Rice on Soil Microorganism Quantity and Transfer of Exogenous Genes of Rice[J]. Chinese Agricultural Science Bulletin, 2020, 36(9): 56-64.

Figures/Tables 9

References 40

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DNA片段	序列		PCR产物/bp
DNA片段	上游引物	下游引物	PCR产物/bp
cry1C^*	5′TTCTACTGGGGAGGACATCG3′<break/>5′CGGTATCTTTGGGTGATTGG3′	5′CGGTATCTTTGGGTGATTGG3′	602
cry2A^*	5′CGTGTCAATGCGGACCTGAT3′<break/>5′GATGCCGGACAGGATGTAGT3′	5′GATGCCGGACAGGATGTAGT3′	600
bar	5′TCTCGGTGACGGGCAGGAC3′<break/>5′CCTGGAAGGCACGCAACG3′	5′CCTGGAAGGCACGCAACG3′	323
P_35S	5′GCTCCTACAAATGCCATCATTGA3′<break/>5′GATAGTGGGATTGTGCGTCATCCC3′	5′GATAGTGGGATTGTGCGTCATCCC3′	195
T_35S	5′GTGGTGTAAACAAATTGACGCTTAG3′<break/>5′GTTTCGCTCATGTGTTGAGCAT3′	5′GTTTCGCTCATGTGTTGAGCAT3′	206
NOS-cry2A^*	5′GAGCTCGAATTTCCCCGATCGTTC3′<break/>5′CCGATCTAGTAACATAGATGACACCG3′	5′CCGATCTAGTAACATAGATGACACCG3′	311
NOS-cry1C^*	5′GAATTTCCCCGATCGTTCAAACA3′<break/>5′CCGATCTAGTAACATAGATGACACCG3′	5′CCGATCTAGTAACATAGATGACACCG3′	305
ubi	5′GGACACCAACCAGCGAACCAG3′<break/>5′GTGCCGTGAGAGGAGGAGGAG3′	5′GTGCCGTGAGAGGAGGAGGAG3′	201
16S rDNA	5′GAGAGTTTGATCCTGGCTCAG3′<break/>5′CTACGGCTACCTTGTTACGA3′	5′CTACGGCTACCTTGTTACGA3′	约1400
18S rDNA	5′GTAGTCATATGCTTGTCTC3′<break/>5′TCCGCAGGTTCACCTACGGA3′	5′TCCGCAGGTTCACCTACGGA3′	约1800

DNA片段	序列		PCR产物/bp
DNA片段	上游引物	下游引物	PCR产物/bp
cry1C^*	5′TTCTACTGGGGAGGACATCG3′<break/>5′CGGTATCTTTGGGTGATTGG3′	5′CGGTATCTTTGGGTGATTGG3′	602
cry2A^*	5′CGTGTCAATGCGGACCTGAT3′<break/>5′GATGCCGGACAGGATGTAGT3′	5′GATGCCGGACAGGATGTAGT3′	600
bar	5′TCTCGGTGACGGGCAGGAC3′<break/>5′CCTGGAAGGCACGCAACG3′	5′CCTGGAAGGCACGCAACG3′	323
P_35S	5′GCTCCTACAAATGCCATCATTGA3′<break/>5′GATAGTGGGATTGTGCGTCATCCC3′	5′GATAGTGGGATTGTGCGTCATCCC3′	195
T_35S	5′GTGGTGTAAACAAATTGACGCTTAG3′<break/>5′GTTTCGCTCATGTGTTGAGCAT3′	5′GTTTCGCTCATGTGTTGAGCAT3′	206
NOS-cry2A^*	5′GAGCTCGAATTTCCCCGATCGTTC3′<break/>5′CCGATCTAGTAACATAGATGACACCG3′	5′CCGATCTAGTAACATAGATGACACCG3′	311
NOS-cry1C^*	5′GAATTTCCCCGATCGTTCAAACA3′<break/>5′CCGATCTAGTAACATAGATGACACCG3′	5′CCGATCTAGTAACATAGATGACACCG3′	305
ubi	5′GGACACCAACCAGCGAACCAG3′<break/>5′GTGCCGTGAGAGGAGGAGGAG3′	5′GTGCCGTGAGAGGAGGAGGAG3′	201
16S rDNA	5′GAGAGTTTGATCCTGGCTCAG3′<break/>5′CTACGGCTACCTTGTTACGA3′	5′CTACGGCTACCTTGTTACGA3′	约1400
18S rDNA	5′GTAGTCATATGCTTGTCTC3′<break/>5′TCCGCAGGTTCACCTACGGA3′	5′TCCGCAGGTTCACCTACGGA3′	约1800

性状	HD1	HD2	HD3	HD4	CK1	CK2
Basta抗感特性	+	+	+	+	–	–
Cry1C-蛋白	+	–	+	–	–	–
Cry2A-蛋白	–	+	–	+	–	–
bar-PCR	+	+	+	+	–	–
cry1C^*-PCR	+	–	+	–	–	–
cry2A^*-PCR	–	+	–	+	–	–

性状	HD1	HD2	HD3	HD4	CK1	CK2
Basta抗感特性	+	+	+	+	–	–
Cry1C-蛋白	+	–	+	–	–	–
Cry2A-蛋白	–	+	–	+	–	–
bar-PCR	+	+	+	+	–	–
cry1C^*-PCR	+	–	+	–	–	–
cry2A^*-PCR	–	+	–	+	–	–