Elevated CO2 Concentrations: Effects on Soil Microbial Quantity and Enzyme Activity in Root Zone of Lycium barbarum

doi:10.11924/j.issn.1000-6850.2020-00015

Abstract

Abstract:

To investigate the effect of atmospheric CO₂ concentration on the soil microenvironment of wolfberry root area in Ningxia, Lycium barbarum L. was used as the test material, and the open-top air chamber was used to simulate the control of CO₂concentration to measure the changes in the number of soil microorganisms and soil enzyme activity in the root zone under the double treatment of atmospheric CO₂concentration. Elevated CO₂ concentration reduced the number of fungi and increased the number of bacteria and actinomycetes. Under 0.5-fold increase CO₂ concentration treatment, the activities of catalase and invertase in wolfberry root area were increased by 24.74% and 23.71%, the urease activity and polyphenol oxidase activity were decreased by 0.45% and 15.29% compared with those of the control. Under double CO₂ concentration treatment, the activities of the three enzymes were increased by 55.74%, 23.07%, and 44.60%, while the polyphenol oxidase decreased by 24.94%. Path analysis showed that the number of fungi had the greatest influence on urease activity, while the number of bacteria had the strongest influence on catalase activity. Atmospheric CO₂ concentration enhanced the correlation between the number of soil microorganisms and enzyme activity in the root zone, in which the number of fungi and bacteria was significantly and negatively correlated with urease, and the number of actinomycetes was significantly and negatively correlated with urease under 0.5-fold treatment. The elevated CO₂ concentration could increase the number of soil bacteria and actinomycetes in the root zone of wolfberry and the activities of soil catalase, urease and invertase.

Key words: CO₂ concentration, Lycium barbarum, soil microorganisms, soil enzyme

CLC Number:

S154

Xie Yun, Guo Fangyun, Cao Bing. Elevated CO₂ Concentrations: Effects on Soil Microbial Quantity and Enzyme Activity in Root Zone of Lycium barbarum[J]. Chinese Agricultural Science Bulletin, 2021, 37(3): 90-97.

Figures/Tables 5

References 39

[1]	IPCC. Climate change 2013: The physical science basis [M]. Cambrige: Cambridge University Press, 2013.
[2]	赵宗慈, 罗勇, 黄建斌. 回顾年—年[J]. 气候变化研究进展, 2018,14(5):540-546.
[3]	Hu H W, Macdonald C, Trivedi P, et al. Effects of climate warming and elevated CO₂ on autotrophic nitrification and nitrifiers in dryland ecosystems[J]. Soil Biology and Biochemistry, 2016,92:1-15. doi: 10.1016/j.soilbio.2015.09.008 URL
[4]	Tfaily M, Hess N, Koyama A, et al. Elevated [CO₂] changes soil organic matter composition and substrate diversity in an arid ecosystem[J]. Geoderma, 2018,330:1-8. doi: 10.1016/j.geoderma.2018.05.025 URL
[5]	Sofi J, Lone A, Ganie M, et al. Soil microbiological activity and carbon dynamics in the current climate change scenarios: a review[J]. Pedosphere, 2016,26(5):577-591.
[6]	Xue S, Yang X, Liu G, et al. Effects of elevated CO₂ and drought on the microbial biomass and enzymatic activities in the rhizospheres of two grass species in Chinese loess soil[J]. Geoderma, 2017,286:25-34. doi: 10.1016/j.geoderma.2016.10.025 URL
[7]	王京伟. 覆膜滴灌对大棚作物根区土壤微环境及作物生长的影响[D]. 杨凌:西北农林科技大学, 2017.
[8]	Wang Y, Yan D, Wang J, et al. Effects of elevated CO₂ and drought on plant physiology, soil carbon and soil enzyme activities[J]. Pedosphere, 2017,27(5):846-855.
[9]	Wu Q, Zhang C, Liang X, et al. Elevated CO₂ improved soil nitrogen mineralization capacity of rice paddy[J]. Science of the Total Environment, 2019,710:1-8.
[10]	Wang Y, Yan D, Wang J, et al. Long-termelevated CO₂ shifts composition of soil microbial communities in a Californian annual grassland, reducing growth and N utilization potentials[J]. Science of the Total Environment, 2019,652:1474-1481. doi: 10.1016/j.scitotenv.2018.10.353 URL
[11]	Kumar A, Nayak A, Das B, et al. Effects of water deficit stress on agronomic and physiological responses of rice and greenhouse gas emission from rice soil under elevated atmospheric CO₂[J]. Sci Total Environ, 2019,650(2):2032-2050. doi: 10.1016/j.scitotenv.2018.09.332 URL
[12]	石元豹, 曹兵, 宋丽华. 浓度倍增对宁夏枸杞种植地土壤养分及微生物的影响[J]. 江苏农业学报, 2016,32(01):201-206.
[13]	Thakur M, Delreal I, Cesarz S, et al. Soil microbial, nematode, and enzymatic responses to elevated CO₂ N fertilization, warming, and reduced precipitation[J]. Soil Biology & Biochemistry, 2019,135:184-193. doi: 10.1016/j.soilbio.2019.04.020 URL
[14]	哈蓉, 马亚平, 曹兵, 等. 模拟浓度升高对宁夏枸杞营养生长与果实品质的影响[J]. 林业科学, 2019,55(6):28-36.
[15]	赵琴, 潘静, 曹兵, 等. 气温升高与干旱胁迫对宁夏枸杞光合作用的影响[J]. 生态学报, 2015,35(18):6016-6022. doi: 10.5846/stxb201401090073 URL
[16]	Ma Y, Reddy V, Devi M, et al. De novo characterization of the Goji berry (Lycium barbarium L.) fruit transcriptome and analysis of candidate genes involved in sugar metabolism under different CO₂ concentrations[J]. Tree Physiology, 2019,39(6):1032-1045. doi: 10.1093/treephys/tpz014 URL pmid: 30824924
[17]	郭芳芸, 哈蓉, 马亚平, 等. 浓度升高对宁夏枸杞苗木光合特性及生物量分配影响[J]. 西北植物学报, 2019,39(2):302-309.
[18]	陆宁海, 杨蕊, 郎剑锋, 等. 秸秆还田对土壤微生物种群数量及小麦茎基腐病的影响[J]. 中国农学通报, 2019,35(34):102-108.
[19]	周礼恺, 张志明. 土壤酶活性的测定方法[J]. 土壤通报, 1980,5:37-38.
[20]	Fanin N, Kardol P, Farrell M, et al. Effects of plant functional group removal on structure and function of soil communities across contrasting ecosystems[J]. Ecology Letters, 2019,22(7):1095-1103. doi: 10.1111/ele.13266 URL pmid: 30957419
[21]	周娅, 冯倩, 王玉, 等. 覆膜玉米不同生育期土壤酶活性对大气浓度升高的响应[J]. 农业环境科学学报, 2019,38(5):1185-1192.
[22]	He W, Moonis M, Chung H, et al. Effects of high soil CO₂ concentrations on seed germination and soil microbial activities[J]. International Journal of Greenhouse Gas Control, 2016,53:117-126. doi: 10.1016/j.ijggc.2016.07.023 URL
[23]	Yang Y, Tilman D, Furey G, et al. Soil carbon sequestration accelerated by restoration of grassland biodiversity[J]. Nature Communications, 2019,10(1):1-7. doi: 10.1038/s41467-018-07882-8 URL pmid: 30602773
[24]	Padhy S, Nayak S, Dash P, et al. Elevated carbon dioxide and temperature imparted intrinsic drought tolerance in aerobic rice system through enhanced exopolysaccharide production and rhizospheric activation[J]. Agriculture, Ecosystems & Environment, 2018,268:52-60.
[25]	Wilschut R, Vander Putten W, Garbeva P, et al. Root traits and belowground herbivores relate to plant-soil feedback variation among congeners[J]. Nature Communcations, 2019,10(1):1-9.
[26]	Mcfarland J, Waldrod M, Haw M. Extreme CO₂ disturbance and the resilience of soil microbial communities[J]. Soil Biology and Biochemistry, 2013,65:274-286. doi: 10.1016/j.soilbio.2013.04.019 URL
[27]	刘磊, 李彩凤, 郭广昊, 等. NaCl+Na₂SO₄胁迫对甜菜根际土壤微生物数量及酶活性的影响[J]. 核农学报, 2016,30(10):2033-2040.
[28]	Luo X, Hou E, Zang X, et al. Effects of elevated atmospheric CO2 and nitrogen deposition on leaf litter and soil carbon degrading enzyme activities in a Cd-contaminated environment: A mesocosm study[J]. Science of the Total Environment, 2019,671:157-164. doi: 10.1016/j.scitotenv.2019.03.374 URL
[29]	吴秀臣, 孙辉, 杨万勤. 土壤酶活性对温度和CO₂浓度升高的响应研究[J]. 土壤, 2007,39(3):358-363.
[30]	辛丽花, 韩士杰, 郑俊强, 等. CO₂浓度升高对土壤微生物及土壤酶影响的研究进展[J]. 土壤通报, 2006,37(6):1231-1235.
[31]	李奕霏, 肖谋良, 袁红朝, 等. CO₂倍增对稻田土壤碳氮水解酶活性的影响[J]. 中国环境科学, 2018,38(9):3474-3480.
[32]	Panneerselvam P, Kumar U, Senapati A, et al. Influence of elevated CO₂ on arbuscular mycorrhizal fungal community elucidated using Illumina MiSeq platform in sub-humid tropical paddy soil[J]. Applied Soil Ecology, 2020,145:1-9.
[33]	田然, 周辉, 黄娟, 等. 大气浓度升高条件下土壤镉污染对土壤酶及微生物群落多样性的影响[J]. 南京大学学报:自然科学版, 2011,47(6):712-717.
[34]	张玉兰, 张丽莉, 陈利军, 等. 稻-麦轮作系统土壤水解酶及氧化还原酶活性对开放式空气浓度增高的响应[J]. 应用生态学报, 2004,15(06):1014-1018.
[35]	马志良, 赵文强, 刘美. 高寒灌丛生长季根际和非根际土壤多酚氧化酶和过氧化氢酶活性对增温的响应[J]. 应用生态学报, 2019,30(11):3681-3688.
[36]	任欣伟, 唐景毅, 柳静臣, 等. 不同氮水平下升高及增温对幼苗土壤酶活性的影响[J]. 北京林业大学学报, 2014,36(5):44-53.
[37]	周娅. 旱作玉米农田土壤酶活性及微生物量碳氮对施氮、覆膜与大气CO₂浓度升高的响应[D]. 杨凌:西北农林科技大学, 2019.
[38]	Zhang Y, Virjamo V, Sobuj N, et al. Elevated temperature and CO₂ affect responses of European aspen (Populus tremula) to soil pyrene contamination[J]. Science of the Total Environment, 2018,634:150-157. doi: 10.1016/j.scitotenv.2018.03.344 URL
[39]	刘远, 潘根兴, 张辉, 等. 大气浓度和温度升高对麦田土壤呼吸和酶活性的影响[J]. 农业环境科学学报, 2017,36(08):1484-1491.

微生物	通径指标	过氧化氢酶	脲酶	转化酶	多酚氧化酶
真菌	过氧化氢酶	0.0240	0.0068	0.0114	-0.0150
	脲酶	-0.1992	-0.6990	-0.3313	0.4362
	转化酶	-0.0120	-0.0199	-0.0420	0.0262
	多酚氧化酶	0.0764	0.1270	-0.1672	0.2680
细菌	过氧化氢酶	0.3710	0.1057	0.1759	-0.2315
	脲酶	-0.1052	-0.3690	-0.1749	0.2303
	转化酶	0.1012	0.1683	0.3550	-0.2215
	多酚氧化酶	-0.0670	-0.1114	0.1466	-0.2350
放线菌	过氧化氢酶	0.4180	0.1191	0.1981	-0.2608
	脲酶	-0.1191	-0.4180	-0.1981	0.2608
	转化酶	0.0812	0.1351	0.2850	-0.1778
	多酚氧化酶	0.0185	0.0308	-0.0406	0.0650
微生物总数量	过氧化氢酶	0.3720	0.1060	0.1763	-0.2321
	脲酶	-0.1055	-0.3700	-0.1754	0.2309
	转化酶	0.1012	0.1683	0.3550	-0.2215
	多酚氧化酶	-0.0664	-0.1104	0.1454	-0.2330

微生物	通径指标	过氧化氢酶	脲酶	转化酶	多酚氧化酶
真菌	过氧化氢酶	0.0240	0.0068	0.0114	-0.0150
	脲酶	-0.1992	-0.6990	-0.3313	0.4362
	转化酶	-0.0120	-0.0199	-0.0420	0.0262
	多酚氧化酶	0.0764	0.1270	-0.1672	0.2680
细菌	过氧化氢酶	0.3710	0.1057	0.1759	-0.2315
	脲酶	-0.1052	-0.3690	-0.1749	0.2303
	转化酶	0.1012	0.1683	0.3550	-0.2215
	多酚氧化酶	-0.0670	-0.1114	0.1466	-0.2350
放线菌	过氧化氢酶	0.4180	0.1191	0.1981	-0.2608
	脲酶	-0.1191	-0.4180	-0.1981	0.2608
	转化酶	0.0812	0.1351	0.2850	-0.1778
	多酚氧化酶	0.0185	0.0308	-0.0406	0.0650
微生物总数量	过氧化氢酶	0.3720	0.1060	0.1763	-0.2321
	脲酶	-0.1055	-0.3700	-0.1754	0.2309
	转化酶	0.1012	0.1683	0.3550	-0.2215
	多酚氧化酶	-0.0664	-0.1104	0.1454	-0.2330

处理	指标	过氧化氢酶	脲酶	转化酶	多酚氧化酶	真菌	细菌	放线菌	微生物总数量
CK	过氧化氢酶	1
	脲酶	0.451	1
	转化酶	0.008	0.221	1
	多酚氧化酶	-0.734^*	-0.440	-0.111	1
	真菌	-0.282	-0.771^*	0.056	0.381	1
	细菌	-0.532	-0.529	-0.152	0.186	0.567	1
	放线菌	-0.129	-0.496	0.136	0.335	0.773	0.433	1
	微生物总数量	-0.531	-0.532	-0.150	0.188	0.572	1.000^*	0.440	1
TR1	过氧化氢酶	1
	脲酶	0.024	1
	转化酶	-0.412	0.240	1
	多酚氧化酶	-0.136	-0.128	0.220	1
	真菌	-0.006	-0.743^*	-0.241	0.408	1
	细菌	0.062	-0.697^*	-0.228	0.011	0.685^*	1
	放线菌	-0.281	-0.681^*	-0.117	0.152	0.810^*	0.720^*	1
	微生物总数量	0.058	-0.700^*	-0.227	0.013	0.689^*	1.000^*	0.726^*	1
TR2	过氧化氢酶	1
	脲酶	-0.321	1
	转化酶	-0.013	-0.222	1
	多酚氧化酶	-0.265	0.318	0.498	1
	真菌	0.130	-0.815^*	0.099	-0.141	1
	细菌	0.187	-0.832^*	0.279	-0.403	0.724^*	1
	放线菌	0.410	-0.495	0.168	-0.102	0.409	0.553	1
	微生物总数量	0.189	-0.832^*	0.279	-0.402	0.724^*	1.000^*	0.559	1

处理	指标	过氧化氢酶	脲酶	转化酶	多酚氧化酶	真菌	细菌	放线菌	微生物总数量
CK	过氧化氢酶	1
	脲酶	0.451	1
	转化酶	0.008	0.221	1
	多酚氧化酶	-0.734^*	-0.440	-0.111	1
	真菌	-0.282	-0.771^*	0.056	0.381	1
	细菌	-0.532	-0.529	-0.152	0.186	0.567	1
	放线菌	-0.129	-0.496	0.136	0.335	0.773	0.433	1
	微生物总数量	-0.531	-0.532	-0.150	0.188	0.572	1.000^*	0.440	1
TR1	过氧化氢酶	1
	脲酶	0.024	1
	转化酶	-0.412	0.240	1
	多酚氧化酶	-0.136	-0.128	0.220	1
	真菌	-0.006	-0.743^*	-0.241	0.408	1
	细菌	0.062	-0.697^*	-0.228	0.011	0.685^*	1
	放线菌	-0.281	-0.681^*	-0.117	0.152	0.810^*	0.720^*	1
	微生物总数量	0.058	-0.700^*	-0.227	0.013	0.689^*	1.000^*	0.726^*	1
TR2	过氧化氢酶	1
	脲酶	-0.321	1
	转化酶	-0.013	-0.222	1
	多酚氧化酶	-0.265	0.318	0.498	1
	真菌	0.130	-0.815^*	0.099	-0.141	1
	细菌	0.187	-0.832^*	0.279	-0.403	0.724^*	1
	放线菌	0.410	-0.495	0.168	-0.102	0.409	0.553	1
	微生物总数量	0.189	-0.832^*	0.279	-0.402	0.724^*	1.000^*	0.559	1

Elevated CO₂ Concentrations: Effects on Soil Microbial Quantity and Enzyme Activity in Root Zone of Lycium barbarum

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