Abstract:

The research aims to provide basic materials for the inducing regeneration of seedlings, and lay a foundation for the breeding of green bean (Phaseolus vulgaris L.). Using green bean asexual cells (stem, leaf, cotyledon, etc.) as experiment materials, the loose callus cell clusters which were mainly based on globular cells with dense cytoplasm were obtained by screening phytohormones and their optimum combination. Finally, the “Induction Medium I” (MS +2,4-D 1 mg/L+ sucrose 30 g/L) which could induce asexual cells to produce common callus was screened. Moreover, we obtained the “Induction Medium II” (MS+6-BA 0.25 mg/L+2,4-D 0.5 mg/L+ AgNO₃10 mg/L) which could induce and maintain the loose embryogenic callus. In this research, we screened a series of media which could induce asexual cells to loose embryogenic cell clusters and maintain embryogenic cell proliferation, solved the problem of callus darkening during induction, and established a new method to induce loose embryogenic callus.

Key words: green bean, loose embryonic callus, induction medium, maintain medium, callus darkening