Regeneration and Genetic Transformation System of Phaseolus vulgaris: Research Advances

doi:10.11924/j.issn.1000-6850.casb19010082

Abstract

Abstract: High-efficient and stable regeneration system and genetic transformation system are required for molecular breeding and functional genomics analysis of common bean. The regeneration system and Agrobacterium- mediated transformation of common beans at home and abroad in recent years were summarized. Firstly, the regeneration methods and research status of common bean were introduced, including the establishment of regeneration system based on organogenesis and somatic embryogenesis and related impact factors. Secondly, the research progress of Agrobacterium-mediated transformation in common bean was summarized, and the main factors affecting the efficiency of Agrobacterium- mediated transformation were analyzed, including the types of receptor explants and the damage of explants, Agrobacterium strain and bacterial concentration, co- culture conditions (time, temperature, the time of illumination), acetosyringone concentration, etc. Then the progress of gene gun-mediated transformation of common bean was reviewed. Finally, the problems in current regeneration system and genetic transformation system of common bean were summarized. At the same time, the future research directions and ideas were predicted, in order to provide references for the establishment of high efficient and stable regeneration system and genetic transformation system of common bean.

CLC Number:

S643.1

References

[1] 聂楚楚,韩玉珠．中国菜豆育种研究进展[J]．长江蔬菜,2011(2)：1-5．
[2] CROCOMO O J, SHARP W R, PETERS J E. Plantlet morphogenesis and the control of callus growth and root induction of phaseolus vulgaris with the addition of a bean seed extract 1[J]. Zeitschrift Für Pflanzenphysiologie, 1976, 78(5): 456-460.
[3] 张欣,付亚萍,周君莉,等．水稻规模化转基因技术体系构建与应用[J]．中国农业科学,2014,47(21)：4141-4154．
[4] 刘允军,贾志伟,刘艳,等．玉米规模化转基因技术体系构建及其应用[J]．中国农业科学,2014,47(21)：4172-4182．
[5] 叶兴国,徐惠君,杜丽璞,等．小麦规模化转基因技术体系构建及其应用[J]．中国农业科学,2014,47(21)：4155-4171．
[6] MALIK K A, SAXENA P K. Regeneration in phaseolus vulgaris l.: High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron[J]. Planta, 1992, 186(3): 384-389.
[7] DELGADO-SANCHEZ P, SAUCEDO-RUIZ M, GONZALEZ-CHAVIRA M, et al. An organogenic plant regeneration system for common bean (Phaseolus vulgaris L.)[J]. Plant Science, 2006, 170(4): 822-827.
[8] MUKESHIMANA, GERARDINE, KELLY, et al. Factors influencing regeneration and Agrobacterium tumefaciens-mediated;transformation of common bean (Phaseolus vulgaris L.)[J]. Plant Biotechnology Reports, 2013, 7(1): 59-70.
[9] DANG W, WEI Z M. High frequency plant regeneration from the cotyledonary node of common bean[J]. Biologia Plantarum, 2009, 53(2): 312-316.
[10] AHMED E E, BISZTRAY G D, VELICH I. Plant regeneration from seedling explants of common bean: Phaseolus vulgaris L[J]. ACTA Biologica Szegediensis, 2002, 46(3-4): 27-28.
[11] FRANKLIN C I, TRIEU T N, GONZALES R A, et al. Plant regeneration from seedling explants of green bean ( Phaseolus vulgaris L) via organogenesis[J]. Plant Cell Tissue Organ Culture, 1991, 24(3): 199-206.
[12] 朱有光,奚惕．菜豆的组织培养和植株再生[J]．植物生理学通讯,1988(02)：60．
[13] ZAMBRE M A, CLERCQ J D, VRANOVá E, et al. Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A. Gray (tepary bean)[J]. Plant Cell Reports, 1998, 17(8): 626-630.
[14] KWAPATA K, SABZIKAR R, STICKLEN M B, et al. In vitro regeneration and morphogenesis studies in common bean[J]. Plant Cell Tissue Organ Culture, 2010, 100(1): 97-105.
[15] SAXENA M K. Regeneration in phaseolus vulgaris l.: promotive role of N6-benzylaminopurine in cultures from juvenile leaves[J]. Planta, 1991, 184(1): 148-150.
[16] CARVALHO M D, LE B V, ZUILY-FODIL Y, et al. Efficient whole plant regeneration of common bean ( Phaseolus vulgaris L.) using thin-cell-layer culture and Silver nitrate[J]. Plant Science, 2000, 159(2): 223-232.
[17] 董少鸣．愈伤组织培养实验中几种外植体的选择[J]．河北民族师范学院学报,2007,27(2)：62-62．
[18] KLU G P, JACOBSEN E, HARTEN A V. Induced mutations in winged bean (Psophocarpus tetragonolobus L. DC) with low tannin content[J]. Euphytica, 1997, 98(1-2): 99-107.
[19] COLLADO R, BERMúDEZ-CARABALLOSO I, GARCíA L R, et al. Epicotyl sections as targets for plant regeneration and transient transformation of common bean using Agrobacterium tumefaciens[J]. In Vitro Cellular Developmental Biology - Plant, 2016, 52(5): 1-12.
[20] MOHAMED M F, CAO J, EARLE E D. Toward production of genetically modified common bean via Agrobacterium-mediated transformation[J]. Annual REPORT-BEAN Improvement Cooperative, 2006, 49: 147.
[21] 刘宏宇,秦智伟．菜豆的组织培养、遗传转化及分子生物学研究进展[C]//蔬菜分子育种研讨会．中国北京,中国北京,2004：6．
[22] SVETLEVA D, VELCHEVA M, BHOWMIK G. Biotechnology as a useful tool in common bean ( Phaseolus vulgaris L.) improvement[J]. Euphytica, 2003, 131(2): 189-200.
[23] VELTCHEVA M R, LILOVA SVETLEVA D. In vitro regeneration of Phaseolus vulgaris L. via organogenesis from petiole explants[J]. Journal of Central European Agriculture, 2005, 6(1): 53-58.
[24] RAVNIKAR M, RODE J, GOGALA N, et al. Regulation of organogenesis with jasmonic acid[C]//[Poster]., 1990: 169-172.
[25] BENEDICIC D, RAVNIKAR M, GOGALA N. The influence of jasmonic acid on the development of Phaseolus vulgaris shoot culture[C]//International Symposium on Plant Biotechnology and its Contribution to Plant Development, Multiplication and Improvement 289, 1991: 85-86.
[26] BENEDI?I? D, RAVNIKAR M, GOGALA N. The regeneration of bean plants from meristem culture[J]. Phyton; Annales REI Botanicae, 1997, 37(1): 151-160.
[27] BRASILEIRO A M, ARAG?O F L, ROSSI S, et al. Susceptibility of common and tepary beans to agrobacterium spp. strains and improvement of Agrobacterium-mediated transformation using microprojectile bombardment[J]. Journal of the American Society for Horticultural Science American Society for Horticultural Science, 1996, 121(5): 810-815.
[28] MOHAMED M F, COYNE D P, READ P E. Shoot organogenesis in callus induced from pedicel explants of common bean (Phaseolus vulgaris L.)[J]. Journal of the American Society for Horticultural Science, 1993, 118(1): 158-162.
[29] ARELLANO J, FUENTES S I, CASTILLO-ESPA?A P, et al. Regeneration of different cultivars of common bean ( Phaseolus vulgaris L.) via indirect organogenesis[J]. Plant Cell Tissue Organ Culture, 2009, 96(1): 11-18.
[30] COLLADO R, VEITíA N, BERMúDEZCARABALLOSO I, et al. Efficient in vitro plant regeneration via indirect organogenesis for different common bean cultivars[J]. Scientia Horticulturae, 2013, 153(3): 109-116.
[31] 张倩．难以再生菜豆不定芽的诱导[D]．南京：南京师范大学,2017．
[32] ALLAVENA A, ROSSETTI L. Efforts in somatic embryogenesis of Phaseolus vulgaris L[J]. ACTA Horticulturae, 1982, 131(131): 239-246.
[33] MALIK K A, SAXENA P K. Somatic embryogenesis and shoot regeneration from intact seedlings of Phaseolus acutifolius A., P. aureus (L.) Wilczek, P. coccineus L., and P. wrightii L[J]. Plant Cell Reports, 1992, 11(3): 163-168.
[34] GARCíA L R, PéREZ J, KOSKY R G, et al. Plant regeneration via somatic embryogenesis in Phaseolus acutifolius A. Gray[J]. Biotecnología Vegetal, 2010, 10(3): 143-149.
[35] COLLADO R, GARCíA L R, ANGENON G, et al. Somatic embryos formation from immature cotyledons in Phaseolus vulgaris cv. CIAP 7247[J]. Biotecnología Vegetal, 2011, 11(4): 235-240.
[36] MARTINS I S, SONDAHL M R. Early stages of somatic embryo differentiation from callus cells of bean ( phaseolus vulgaris l.) grown in liquid medium[J]. Journal of Plant Physiology, 1984, 117(2): 97-103.
[37] CABRERA-PONCE J L, LóPEZ L, LEóN-RAMíREZ C G, et al. Stress induced acquisition of somatic embryogenesis in common bean Phaseolus vulgaris L[J]. Protoplasma, 2015, 252(2): 559-570.
[38] MARIOTTI D, FONTANA G S, SANTINI L. Genetic transformation of grain legumes: Phaseolus vulgaris L. and P. coccineus L[J]. J Genet Breed, 1989, 43: 77-82.
[39] DILLEN W, DE CLERCQ J, GOOSSENS A, et al. Exploiting the presence of regeneration capacity in the Phaseolus gene pool for Agrobacterium-mediated gene transfer to the common bean[J]. Mededelingen Van DE Faculteit Landbouwkundige EN Toegepaste Biologische Wetenschappen, Universiteit GENT, 1997, 62(4a): 1397-1402.
[40] ZHANG Z, COYNE D P, MITRA A. Factors affecting Agrobacterium-mediated transformation of common bean[J]. Journal of the American Society for Horticultural Science American Society for Horticultural Science, 1997, 122(3): 300-305.
[41] ESTRADA-NAVARRETE G, DIAZ-CAMINO C, SANTANA O, et al. Agrobacterium rhizogenes transformation of the Phaseolus spp.: a tool for functional genomics[J]. Molecular plant-microbe Interactions : MPMI, 2006, 19(12): 1385-1393.
[42] GEORGINA E N, XOCHITL A A, JUAN-ELíAS O, et al. Fast, efficient and reproducible genetic transformation of Phaseolus spp. by Agrobacterium rhizogenes[J]. Nature Protocols, 2007, 2(7): 1819-1824.
[43] AMUGUNE N O, ANYANGO B, MUKIAMA T K. Arobacterium-mediated transformation of common bean[J]. African Crop Science Journal, 2011, 19(3): 137-147.
[44] COLLADO R, BERMúDEZ-CARABALLOSO I, GARCíA L R, et al. Agrobacterium -mediated transformation of Phaseolus vulgaris L. using indirect organogenesis[J]. Scientia Horticulturae, 2015, 195(27): 89-100.
[45] NAGL W, IGNACIMUTHU S, BECKER J. Genetic engineering and regeneration of Phaseolus and Vigna . State of the art and new attempts[J]. Journal of Plant Physiology, 1997, 150(6): 625-644.
[46] GHORBEL R, DOMíNGUEZ A, NAVARRO L, et al. High efficiency genetic transformation of sour orange (Citrus aurantium) and production of transgenic trees containing the coat protein gene of citrus tristeza virus[J]. Tree Physiology, 2000, 20(17): 1183-1189.
[47] CERVERA M, PINA J A, JUáREZ J, et al. A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype[J]. Theoretical Applied Genetics, 2000, 11(12): 1683-1685.
[48] BOND J E, ROOSE M L. Agrobacterium-mediated transformation of the commercially important citrus cultivar Washington navel orange[J]. Plant Cell Reports, 1998, 18(3-4): 229-234.
[49] ZAMBRE, GOOSSENS, CARDONA, et al. A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil[J]. Theoretical Applied Genetics, 2005, 110(5): 914-924.
[50] GURLITZ R H, LAMB P W, MATTHYSSE A G. Involvement of carrot cell surface proteins in attachment of agrobacterium tumefaciens[J]. Plant Physiology, 1987, 83(3): 564-568.
[51] ESPINOSA-HUERTA E, QUINTERO-JIMéNEZ A, CABRERA-BECERRA K, et al. Stable and efficient Agrobacterium tumefaciens mediated transformation of Phaseolus vulgaris[J]. Agrociencia, 2013, 47(4): .
[52] DILLEN W, CLERCQ J D, GOOSSENS A, et al. Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray[J]. Theoretical Applied Genetics, 1997, 94(2): 151-158.
[53] OBERPICHLER I, ROSEN R, RASOULY A, et al. Light affects motility and infectivity of Agrobacterium tumefaciens[J]. Environmental Microbiology, 2010, 10(8): 2020-2029.
[54] MUKUND Z, NANCY T, JANNIEK D C, et al. Light strongly promotes gene transfer from Agrobacterium tumefaciens to plant cells[J]. Planta, 2003, 216(4): 580-586.
[55] ZUPAN J, MUTH T R, AND O D, et al. The transfer of DNA from Agrobacterium tumefaciens into plants: a feast of fundamental insights[J]. Plant Journal, 2010, 23(1): 11-28.
[56] 刘海坤,卫志明．大豆遗传转化研究进展[J]．分子植物(英文版),2005,31(2)：126-134．
[57] DINKINS R D, REDDY M S, MEURER C A, et al. Recent advances in soybean transformation[J]. Plant Tissue Culture Biotechnology, 2003, 3: 9-26.
[58] RUSSELL D R, WALLACE K M, BATHE J H, et al. Stable transformation of Phaseolus vulgaris via electric-discharge mediated particle acceleration[J]. Plant Cell Reports, 1993, 12(3): 165-169.
[59] KIM J W, MINAMIKAWA T. Transformation and regeneration of French bean plants by the particle bombardment process[J]. Plant Science, 1996, 117(1-2): 131-138.
[60] KIM J W, MINAMIKAWA T. Stable delivery of a canavalin Promoter-β-Glucuronidase gene fusion into French bean by particle bombardment[J]. Plant Cell Physiology, 1997, 38(1): 70-75.
[61] ARAGAO F J, ALMEIDA E R, GANDER E S, et al. Particle bombardment-mediated transient expression of a Brazil nut methionine-rich albumin in bean (Phaseolus vulgaris L.)[J]. Plant Molecular Biology, 1992, 20(2): 357-359.
[62] ARAG?O F L, BARROS L G, BRASILEIRO A M, et al. Inheritance of foreign genes in transgenic bean ( Phaseolus vulgaris L.) co-transformed via particle bombardment[J]. Theoretical Applied Genetics, 1996, 93(1-2): 142-150.
[63] ARAG?O F L, BARROS L G, DE SOUSA M V, et al. Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa HBK, Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)[J]. Genetics and Molecular Biology, 1999, 22(3): 445-449.
[64] NGUYEN T, STICKLEN M. Genetic transformation of common bean (Phaseolus vulgaris L.) with the gus color marker, the bar herbicide resistance, and the barley (Hordeum vulgare) HVA1 drought tolerance genes[J]. International Journal of Agronomy, 2012.