Proanthocyanidin Extraction Process from Brown Cotton Fiber: Optimized by Response Surface Method

doi:10.11924/j.issn.1000-6850.casb2020-0379

Abstract

Abstract:

To improve the yield of proanthocyanidin from brown cotton fiber, and optimize the extraction process, the brown cotton fibers were used as materials to study the effect of methanol concentration, solid-liquid ratio, ultrasonic time and temperature on the extraction efficiency of proanthocyanidin, and to obtain the optimized extraction conditions. Proanthocyanidin was extracted by ultrasonic combined with methanol-n-butanol hydrochloride, the extraction process was optimized by response surface methodology. The results showed that the optimum parameters were methanol concentration of 83%, solid-liquid ratio of 1:60, ultrasonic temperature of 42℃, ultrasonic time of 18 min, and the extraction rate was (7.16±0.03)%. The extraction yield of proanthocyanidin by the optimized method was improved by about 4% compared with that of the general method. The extraction process optimized by response surface methodology has high extraction efficiency, and the experimental operation is simple and fast, which can be used to extract proanthocyanidin in batches. The study lays a foundation for the research on biological activities and the development of products of proanthocyanidin.

Key words: brown cotton, cotton fiber, proanthocyanidin, single factor, response surface method, extraction process

CLC Number:

Xie Yongfei, Zhao Gang, Sun Na, Lv Yinping, Wu Yong, Shen Meichen, Lv Wenben, Gao Junshan. Proanthocyanidin Extraction Process from Brown Cotton Fiber: Optimized by Response Surface Method[J]. Chinese Agricultural Science Bulletin, 2021, 37(12): 136-143.

Figures/Tables 9

References 34

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来源	平方和	自由度	均方	F值	P值	显著性
Model	75.30	14	5.38	3.00	0.0244^*	significant
A-甲醇浓度	6.27	1	6.27	3.49	0.0827
B-超声时间	3.27	1	3.27	1.82	0.1987
C-超声温度	33.84	1	33.84	18.86	0.0007^**
D-料液比	0.96	1	0.96	0.53	0.4767
AB	0.37	1	0.37	0.20	0.6581
AC	2.23	1	2.23	1.24	0.2837
AD	0.35	1	0.35	0.20	0.6637
BC	0.33	1	0.33	0.19	0.6731
BD	0.54	1	0.54	0.30	0.5919
CD	0.14	1	0.14	0.080	0.7815
A2	14.25	1	14.25	7.94	0.0137^*
B2	0.99	1	0.99	0.55	0.4702
C2	11.38	1	11.38	6.34	0.0246^*
D2	11.19	1	11.19	6.24	0.0256^*
Residual	25.13	14	1.79
Lack of Fit	22.19	10	2.22	3.02	0.1489	Not significant
Pure Error	2.94	4	0.73
Cor Total	100.43

来源	平方和	自由度	均方	F值	P值	显著性
Model	75.30	14	5.38	3.00	0.0244^*	significant
A-甲醇浓度	6.27	1	6.27	3.49	0.0827
B-超声时间	3.27	1	3.27	1.82	0.1987
C-超声温度	33.84	1	33.84	18.86	0.0007^**
D-料液比	0.96	1	0.96	0.53	0.4767
AB	0.37	1	0.37	0.20	0.6581
AC	2.23	1	2.23	1.24	0.2837
AD	0.35	1	0.35	0.20	0.6637
BC	0.33	1	0.33	0.19	0.6731
BD	0.54	1	0.54	0.30	0.5919
CD	0.14	1	0.14	0.080	0.7815
A2	14.25	1	14.25	7.94	0.0137^*
B2	0.99	1	0.99	0.55	0.4702
C2	11.38	1	11.38	6.34	0.0246^*
D2	11.19	1	11.19	6.24	0.0256^*
Residual	25.13	14	1.79
Lack of Fit	22.19	10	2.22	3.02	0.1489	Not significant
Pure Error	2.94	4	0.73
Cor Total	100.43