Abstract:

The aims are to analyze the biological function of SlMYB44 gene and provide a scientific basis for exploring its mechanism of resisting chilling injury. In this study, the Russian cultivated tomato bolgogragsky was used as the material, and according to the sequencing results of transcriptome, the differentially expressed gene SlMYB44 was selected as the research object. The conservative functional domain, hydrophilicity or hydrophobicity, signal peptide and promoter cis-acting elements of tomato SlMYB44 gene were analyzed by using various bioinformatics databases. The expression vector pMDC43-MYB44 was constructed for subcellular localization analysis. SlMYB44 gene encoded 372 amino acids with a total length of 1551 bp. SlMYB44 protein had no potential signal peptide site and membrane spaning domain, which belonged to labile protein and hydrophilic protein. The analysis of cis-acting elements of SlMYB44 gene promoter showed that most of them were related to adversity stress and hormone response. Subcellular localization analysis demonstrated that the expression product of SlMYB44 gene was localized in the cell nucleus. In conclusion, the SlMYB44 gene responds to chilling injury, it is further speculated that it might affect the tomato chilling mechanism.

Key words: Solanum lycopersicum, gene, SlMYB44, bioinformatics analysis, subcellular localization, low temperature stress