α-Linolenic Acid from Perilla Oil: Purification Effect Based on Molecular Distillation Technology

doi:10.11924/j.issn.1000-6850.casb2021-0130

Abstract

Abstract:

In order to explore the purification feasibility of α-linolenic acid from perilla oil based on molecular distillation technology, based on screening the best extraction method of perilla oil, the effects of four factors of distillation temperature, distillation pressure, scraper speed and feed speed on purification of α-linolenic acid were studied. The results show that the supercritical CO₂ extraction method is the best for perilla oil, and the primary factor affecting the purity of α-linolenic acid is distillation temperature, followed by distillation pressure, feed speed and scraper speed. The optimal purification process is as follows: distillation temperature of 220℃, distillation pressure of 0.07 mbar, scraper speed of 375 r/min, feed speed of 1.0 d/min, under this process condition, the purity of α-linolenic acid could reach 97.42%. This method significantly improves the purity of α-linolenic acid from perilla oil, and could lay a theoretical foundation for the research and utilization of highly purified α-linolenic acid.

Key words: perilla oil, molecular distillation technology, separation and purification, extract method, α-linolenic acid

CLC Number:

Liu Ming, Tang Jiawen, Sha Shuang, Meng Jianxia, Cheng Li, Li Chongwei. α-Linolenic Acid from Perilla Oil: Purification Effect Based on Molecular Distillation Technology[J]. Chinese Agricultural Science Bulletin, 2021, 37(33): 135-141.

Figures/Tables 8

References 33

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编号	A蒸馏温度/℃	B蒸馏压力/mbar	C刮板转速/(r/min)	D进料速度/(d/min)
1	200	0.05	375	0.5
2	210	0.06	400	1.0
3	220	0.07	425	1.5

编号	A蒸馏温度/℃	B蒸馏压力/mbar	C刮板转速/(r/min)	D进料速度/(d/min)
1	200	0.05	375	0.5
2	210	0.06	400	1.0
3	220	0.07	425	1.5

提取方法	提取率/%	碘值/(g/100g)	酸值/(mg/g)	皂化值/(mg/g)	折光率	α-亚麻酸纯度/%
索氏抽提法	49.28±1.11a	162.99±1.35d	2.32±0.08a	197.05±3.09d	1.46±0.02ab	47±1.64c
冷榨法	34.15±1.07c	171.15±3.21b	2.13±0.08b	199.53±2.76c	1.46±0.01ab	52±1.07b
热榨法	36.83±1.07b	169.13±2.77c	1.87±0.06c	206.47±2.24b	1.43±0.01b	54±1.15b
超临界萃取法	31.70±1.40d	189.51±2.46a	1.98±0.04c	215.00±2.13a	1.48±0.02a	59±1.31a

提取方法	提取率/%	碘值/(g/100g)	酸值/(mg/g)	皂化值/(mg/g)	折光率	α-亚麻酸纯度/%
索氏抽提法	49.28±1.11a	162.99±1.35d	2.32±0.08a	197.05±3.09d	1.46±0.02ab	47±1.64c
冷榨法	34.15±1.07c	171.15±3.21b	2.13±0.08b	199.53±2.76c	1.46±0.01ab	52±1.07b
热榨法	36.83±1.07b	169.13±2.77c	1.87±0.06c	206.47±2.24b	1.43±0.01b	54±1.15b
超临界萃取法	31.70±1.40d	189.51±2.46a	1.98±0.04c	215.00±2.13a	1.48±0.02a	59±1.31a

因素水平	A蒸馏温度/℃	B蒸馏压力/mbar	C刮板转速/(r/min)	D进料速度/(d/min)	α-亚麻酸纯度/%
1	1	1	1	1	85.07±0.21
2	1	2	2	2	87.21±1.20
3	1	3	3	3	89.14±0.56
4	2	1	2	3	85.81±0.49
5	2	2	3	1	88.52±0.85
6	2	3	1	2	93.23±0.37
7	3	1	3	2	91.42±0.70
8	3	2	1	3	93.08±0.68
9	3	3	2	1	97.12±0.25
k₁	87.14	87.43	90.46	90.24
k₂	89.19	89.61	90.05	90.62
k₃	93.87	93.16	89.69	89.34
R	6.73	5.73	1.16	1.28