Residues of Geosmin and 2- Mathylisoborneol in Aquaculture Water and Aquatic Products Determined by Headspace Solid-phase Microextraction - Gas Chromatography Mass Spectrometry

doi:10.11924/j.issn.1000-6850.casb2021-0404

Abstract

Abstract:

In this paper, we optimized the determination method for geosmin (GSM) and 2-mathylisoborneol (2-MIB) in aquaculture water and aquatic products by using the method of microwave distillation - solid-phase microextraction - gas chromatographic-mass spectrometry (MD-SPME-GC-MS). We optimized the four pre-treatment processing parameters, including carrier gas rate, microwave power, distillation time and collection method, and determined the experimental conditions as follows: carrier gas rate of 60 mL/min, microwave power of 400 W, distillation time of 6 min, and constant of 250 mL. In aquaculture water, the detection limit of GSM and 2-MIB reached 1 ng/L and 10 ng/L, respectively. In aquatic products, the detection limit of GSM and 2-MIB reached 0.025 μg/kg and 0.25 μg/kg, respectively. The standard curve of this method had good liner relation in the range of 1-200 ng/L and 10-200 ng/L (R² was 0.9997 and 0.9982, respectively). The average recovery rate of GSM and 2-MIB at different concentrations (1, 5, 7, 10, and 20 μg/kg) was 45.28%-49.92% and 82.15%-87.36%, respectively. In this experiment, the water samples and aquatic product samples from Wuxi were detected by this method. The result showed that the average concentration of GSM and 2-MIB in the water samples was 19.58 ng /L and 58.29 ng /L, respectively. In aquatic products, only GSM was detected, and the average concentration was 0.61 μg/kg. This method can be widely used to detect fishy substances in aquaculture water and aquatic products.

Key words: aquaculture, geosmin, 2-mathylisoborneo1, headspace solid-phase microextraction

CLC Number:

S912

ZOU Jianmin, LU Qi, GUI Yuan, SONG Chao, CHEN Jiazhang. Residues of Geosmin and 2- Mathylisoborneol in Aquaculture Water and Aquatic Products Determined by Headspace Solid-phase Microextraction - Gas Chromatography Mass Spectrometry[J]. Chinese Agricultural Science Bulletin, 2022, 38(35): 148-156.

Figures/Tables 10

References 36

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化合物	保留时间/min	定性离子/(m/z)	定量离子/(m/z)
GSM	12.303	112，125	112
2-MIB	9.184	95，108，135	95

化合物	保留时间/min	定性离子/(m/z)	定量离子/(m/z)
GSM	12.303	112，125	112
2-MIB	9.184	95，108，135	95

土腥味化合物	加标浓度/(μg/kg)	平均回收率/%	相对标准偏差/%
2-MIB	1	0	0
	5	86.29	21.65
	7	87.36	6.78
	10	82.15	5.24
	20	84.62	7.53
GSM	1	48.31	2.59
	5	49.14	18.6
	7	49.92	7.48
	10	45.28	2.15
	20	48.71	7.56

土腥味化合物	加标浓度/(μg/kg)	平均回收率/%	相对标准偏差/%
2-MIB	1	0	0
	5	86.29	21.65
	7	87.36	6.78
	10	82.15	5.24
	20	84.62	7.53
GSM	1	48.31	2.59
	5	49.14	18.6
	7	49.92	7.48
	10	45.28	2.15
	20	48.71	7.56