Plant Aqueous Extract of Momordica charantia: Effects on Rice Seed Germination and Seedling Growth

doi:10.11924/j.issn.1000-6850.casb2021-0433

Abstract

Abstract:

The aim is to clarify the allelopathic potential of Momordica charantia plants on succeeding rice and provide guidance for the application of rice-vegetable rotation system. The experiment was conducted with plant residues of M. charantia as experimental materials, the allelopathic effects of its aqueous extract on seed germination and seedling growth of three rice varieties (‘Texianzhan 25’, ‘BoⅡyou 818’ and ‘Ⅱyou 129’) were studied by petri dish and water culture methods. The results showed that the germination rates of the three rice varieties’ seeds were reduced when the concentration of aqueous extract only reached 0.20 g/mL, the seed vigor indexes of ‘BoⅡyou 818’ and ‘Ⅱyou 129’ were reduced, and the average germination time of ‘Texianzhan 25’ was prolonged by 1.13 days, but the germination inhibition rate was less than 20% and the seed vigor index inhibition rate was less than 30%. Although the root elongation of ‘Texianzhan 25’ seedlings was inhibited by dose-dependent effect, the aqueous extract was beneficial to the distribution of the total biomass of the three rice varieties’ seedlings to the aboveground parts. The aqueous extract with concentration≤0.15 g/mL had no effect or promoting effect on seedling height and total biomass, and the inhibition rate of total biomass was less than 28% when the concentration reached 0.20 g/mL. Therefore, the allelopathic inhibition effect of M. charantia on succeeding rice is weak.

Key words: Momordica charantia, seed vigor index, average germination time, rice seedling growth, biomass allocation

CLC Number:

S642.5

LI Xuefeng, WANG Jian, YE Xiaoyuan, ZHANG Xiuting, WANG Lixue. Plant Aqueous Extract of Momordica charantia: Effects on Rice Seed Germination and Seedling Growth[J]. Chinese Agricultural Science Bulletin, 2022, 38(6): 1-7.

Figures/Tables 6

References 38

[1]	蔡尤俊, 沈嘉伟, 刁石新, 等. 稻菜轮作对稻田褐飞虱和蜘蛛数量的影响[J]. 环境昆虫学报, 2015, 37(3):548-550.
[2]	张振飞, 黄炳超, 肖汉祥, 等. 4种农业措施对三化螟种群动态的控制作用[J]. 生态学报, 2013, 33(22):7173-7180.
[3]	程传鹏, 潘俊峰, 万开元, 等. 轮作对农田杂草的影响研究进展[J]. 中国农学通报, 2013, 29(30):1-9.
[4]	海南省统计局国家统计局海南调查总队编. 海南统计年鉴2018[M]. 北京: 中国统计出版社, 2018.
[5]	SUN B, ZHANG L X, YANG L Z, et al. Agricultural non-point source pollution in China: causes and mitigation measures[J]. Ambio, 2012, 41(4):370-379. doi: 10.1007/s13280-012-0249-6 URL
[6]	ZHANG G S, LI J C, HU X B, et al. On-farm assessment of soil erosion and non-point source pollution in a rain-fed vegetable production system at Dianchi lake’s catchment, southwestern China[J]. Nutr cycl agroecosyst, 2013, 96(1):67-77. doi: 10.1007/s10705-013-9577-6 URL
[7]	廖小红, 李汉平. 农作物秸秆COD负荷测定及中国弃置秸秆可能形成的面源COD总量极大值估算[J]. 农业环境科学学报, 2007, 26(5):1977-1981.
[8]	DAS A, PATEL D P, MUNDA G C, et al. Nutrient recycling potential in rice-vegetable cropping sequences under in situ residue management at mid-altitude subtropical Meghalaya[J]. Nutr cycl agroecosyst, 2008, 82(3):251-258. doi: 10.1007/s10705-008-9184-0 URL
[9]	尚丹, 马颖杰, 邢虎成, 等. 冬闲地套种蔬菜及蔬菜废弃物还田对苎麻的影响[J]. 作物研究, 2014(1):37-42.
[10]	杨岩. 蔬菜废弃物堆肥和原位还田研究[D]. 泰安:山东农业大学, 2015.
[11]	YUN Z, MIN G, KAI S, et al. Effects of aqueous root extracts and hydrophobic root exudates of cucumber (Cucumis sativus L.) on nuclei DNA content and expression of cell cycle-related genes in cucumber radicles[J]. Plant & soil, 2010, 327(1-2):455-463.
[12]	陈淑芳, 曹晓华. 南瓜根系及根部土壤水浸液化感作用研究[J]. 热带作物学报, 2011, 32(12):2268-2273.
[13]	YANG R X, GAO Z G, LIU X, et al. Effects of phenolic compounds of muskmelon root exudates on growth and pathogenic gene expression of Fusarium oxysporum f. sp. melonis[J]. Allelopathy journal, 2015, 35(2):175-186.
[14]	李东阳, 周艳, 叶美菊. 不同产地的苦瓜营养成分分析[J]. 微量元素与健康研究, 2007, 24(5):29-37.
[15]	ZHANG J, HUANG Y, KIKUCHI T, et al. Cucurbitane triterpenoids from the leaves of Momordica charantia, and their cancer chemopreventive effects and cytotoxicities[J]. Chemistry & biodiversity, 2012, 9(2):428-440.
[16]	AHMED I, LAKHANI M S, GILLETT M, et al. Hypotrigly ceridemic and hypocholes-terolernic effects of anti-diabetic Momordica chararaia (Karela) fruit extract in streptozotoein induced diabetic rats[J]. Diabetes research and clinical practice, 2001(51):155-161.
[17]	SCHAEFER H, RENNER S S. A three-genome phylogeny of Momordica (Cucurbitaceae) suggests seven returns from dioecy to monoecy and recent long-distance dispersal to Asia[J]. Molecular phylogenetics and evolution, 2010, 54(2):553-560. doi: 10.1016/j.ympev.2009.08.006 URL
[18]	刘子记, 杨衍. 海南苦瓜主要病虫害的发生及防治技术[J]. 南方农业学报, 2012, 43(10):1501-1504.
[19]	FANG E F, NG T B. Bitter Gourd (Momordica charantia) is a cornucopia of health: a review of its credited antidiabetic, anti-HIV, and antitumor properties[J]. Current molecular medicine, 2011, 11(5):417-436. doi: 10.2174/156652411795976583 URL
[20]	SENANAYAKE G V K, FUKUDA N, NSHIZONO S, et al. Mechanisms underlying decreased hepatic triacylglycerol and cholesterol by dietary bitter melon extract in the rat[J]. Lipids, 2012, 47(5):495-503. doi: 10.1007/s11745-012-3667-0 URL
[21]	黄舒曼, 王宙, 符海秋. 优质水稻特籼占25引种试验示范与推广应用[J]. 农业科技通讯, 2011(10):102-104.
[22]	唐清杰, 严小微, 王惠艰, 等. 优质杂交水稻新组合博Ⅱ优818在海南示范及高产栽培技术[J]. 杂交水稻, 2018, 33(3):44-45.
[23]	曹树青, 翟虎渠, 盛生兰, 等. 高产杂交籼稻Ⅱ优129光合碳同化特性的研究[J]. 杂交水稻, 2001(1):46-50.
[24]	LI X F, WANG J, HUANG D, et al. Allelopathic potential of Artemisia frigida and successional changes of plant communities in the northern China steppe[J]. Plant and soil, 2011, 341(1-2):383-398. doi: 10.1007/s11104-010-0652-3 URL
[25]	孔垂华, 娄永根主编. 化学生态学前言[M]. 北京: 高等教育出版社, 2010.
[26]	CHOU C H. Role of allelopathy in sustainable agriculture: use of allelochemicals as naturally occurring bio-agrochemicals[J]. Allelopathy journal, 2010, 25(1):3-16.
[27]	AMB M K, AHLUWALIA A S. Allelopathy: potential role to achieve new milestones in rice cultivation[J]. Rice science, 2016, 23(4):165-183. doi: 10.1016/j.rsci.2016.06.001 URL
[28]	史云峰, 周娜娜, 黎明, 等. 秸秆还田条件下水稻-瓜菜轮作对土壤肥力的影响[J]. 海南热带海洋学院学报, 2017, 24(5):76-80.
[29]	李栋梁, 严小微, 唐清杰, 等. 优质特种稻在海南中部山区的种植表现及高产栽培技术[J]. 中国稻米. 2018, 24(5):118-120.
[30]	FALSTER D S, WESTOBY M. Plant height and evolutionary games[J]. Trends in ecology and evolution, 2003, 18(7):337-343. doi: 10.1016/S0169-5347(03)00061-2 URL
[31]	MAMOLOS A P, KALBURTJI K L. Significance of allelopathy in crop rotation[J]. Journal of crop production, 2001, 4(2):197-218. doi: 10.1300/J144v04n02_06 URL
[32]	MUTLU S, ATİCİ Ö. Allelopathic effect of Nepeta meyeri Benth. extracts on seed germination and seedling growth of some crop plants[J]. Acta physiologiae plantarum, 2009, 31(1):89-93. doi: 10.1007/s11738-008-0204-0 URL
[33]	LIN K W, YANG S C, LIN C N. Antioxidant constituents from the stems and fruits of Momordica charantia[J]. Food chemistry, 2011, 127(2):609-614. doi: 10.1016/j.foodchem.2011.01.051 URL
[34]	CHANG C L, CHEN C R, LIAO Y W, et al. Cucurbitane-type triterpenoids from Momordica charantia[J]. Journal of natural products, 2006, 69(8):1168-1171. doi: 10.1021/np068008v URL
[35]	GE X H, YU P, ZENG Z L, et al. Separation, identification and its antibacterial activity of momordicineⅠfrom the vine of Momordica charantia L.[J]. Science & technology of food industry, 2015, 36(21):118-121.
[36]	JABRAN K, MAHAJAN G, SARDANA V, et al. Allelopathy for weed control in agricultural systems[J]. Crop protection, 2015, 72:57-65. doi: 10.1016/j.cropro.2015.03.004 URL
[37]	FAROOQ M, NAWAZ A, AHMAD E, et al. Using Sorghum to suppress weeds in dry seeded aerobic and puddled transplanted rice[J]. Field crops research, 2017, 214:211-218. doi: 10.1016/j.fcr.2017.09.017 URL
[38]	KHANH T D, CHUNG M I, XUAN T D, et al. The exploitation of crop allelopathy in sustainable agricultural production[J]. Journal of agronomy & crop science, 2010, 191(3):172-184.

浓度/(g/mL)	受试水稻种类
浓度/(g/mL)	特籼占25	博Ⅱ优818	Ⅱ优129
CK	100.00±23.53 bA	100.00±12.83 aA	100.00±9.62 aA
0.05	147.26±11.42 aA	103.53±6.40 aB	95.17±10.46 aB
0.10	130.58±17.37 aA	96.98±9.18 aB	90.95±9.09 abB
0.15	104.38±14.68 bA	84.25±6.72 bB	81.87±13.07 bB
0.20	95.58±10.83 bA	77.84±11.07 bB	70.32±11.91 cB

浓度/(g/mL)	受试水稻种类
浓度/(g/mL)	特籼占25	博Ⅱ优818	Ⅱ优129
CK	100.00±23.53 bA	100.00±12.83 aA	100.00±9.62 aA
0.05	147.26±11.42 aA	103.53±6.40 aB	95.17±10.46 aB
0.10	130.58±17.37 aA	96.98±9.18 aB	90.95±9.09 abB
0.15	104.38±14.68 bA	84.25±6.72 bB	81.87±13.07 bB
0.20	95.58±10.83 bA	77.84±11.07 bB	70.32±11.91 cB

受试植物	具体部位	浓度
受试植物	具体部位	CK	0.05 g/mL	0.10 g/mL	0.15 g/mL	0.20 g/mL
特籼占25	地上	0 aA	-40.83±7.33 dA	-35.36±7.32 cdB	-29.17±6.50 cB	-16.82±6.04 bB
特籼占25	地下	0 aA	-32.96±9.29 cA	-20.56±6.22 bA	-11.11±5.07 abA	-7.41±4.59 aA
博Ⅱ优818	地上	0 aA	-22.64±5.93 cB	-12.22±3.25 bB	-6.95±3.47 abB	-0.25±0.17 aB
博Ⅱ优818	地下	0 dA	8.54±0.42 cdA	17.16±1.63 bcA	20.17±1.89 bA	34.30±7.51 aA
Ⅱ优129	地上	0 bcA	-8.81±4.35 cB	-4.26±4.51 bcB	3.65±1.16 abB	10.61±2.57 aB
Ⅱ优129	地下	0 cA	13.40±0.82 bA	15.88±1.22 abA	17.87±3.54 abA	23.78±5.32 aA

受试植物	具体部位	浓度
受试植物	具体部位	CK	0.05 g/mL	0.10 g/mL	0.15 g/mL	0.20 g/mL
特籼占25	地上	0 aA	-40.83±7.33 dA	-35.36±7.32 cdB	-29.17±6.50 cB	-16.82±6.04 bB
特籼占25	地下	0 aA	-32.96±9.29 cA	-20.56±6.22 bA	-11.11±5.07 abA	-7.41±4.59 aA
博Ⅱ优818	地上	0 aA	-22.64±5.93 cB	-12.22±3.25 bB	-6.95±3.47 abB	-0.25±0.17 aB
博Ⅱ优818	地下	0 dA	8.54±0.42 cdA	17.16±1.63 bcA	20.17±1.89 bA	34.30±7.51 aA
Ⅱ优129	地上	0 bcA	-8.81±4.35 cB	-4.26±4.51 bcB	3.65±1.16 abB	10.61±2.57 aB
Ⅱ优129	地下	0 cA	13.40±0.82 bA	15.88±1.22 abA	17.87±3.54 abA	23.78±5.32 aA