Study on Anti- Mycoplasma capricolumsubsp capricolum by Yinqin Maxingshigan Decoction and Juqi Maxingshigan Decoction

doi:10.11924/j.issn.1000-6850.casb2021-0924

Abstract

Abstract:

To study the effect of prescriptions on mycoplasma pneumonia of goats, venous blood is collected from goat before and after the infection with Mycoplasma capricolumsubsp capricolum, the serum contents of IL-10, IFN-γ and TNF-α were detected by ELISA. At the same time, the clinical symptoms were observed daily, pathological necropsies and tissue section observation were performed on the goats. The results showed that normal control group had no abnormalities, the positive control group had obvious symptoms and 4 goats died. The clinical symptoms of the prescription treatment groups were significantly improved compared with those of the positive control group, and goats of Yinqin Maxingshigan decoction medium dose group were all cured. In terms of IL-10, IFN-γ and TNF-α, on the 7th day, there was significant difference between negative control group and other 7 groups (P<0.01); on the 14th day, there was significant difference between the negative control group and other 7 groups (P<0.01), significant difference between the positive control group and the 6 groups of Chinese medicine treatment (P<0.01), and significant difference between the medium dose group of Yinqin Maxingshigan decoction and other 5 groups of Chinese medicine treatment (P<0.05). As to IL-10 and IFN-γ, on the 21st day, there was significant difference between negative control group and other 7 groups (P<0.01), significant difference between the positive control group and the 6 groups of Chinese medicine treatment (P<0.01), significant difference between the medium dose group of Yinqin Maxingshigan decoction and other 5 groups of Chinese medicine treatment (P<0.01), and no significant difference between the medium dose group of Yinqin Maxingshigan decoction and negative control group (P>0.05). For TNF-α, on the 21st day, the negative control group had significant difference from the other 7 groups (P<0.01), there was significant difference between the positive control group and the 6 groups of Chinese medicine treatment (P<0.01), significant difference between the high dose group of Juqi Maxingshigan decoction and the other 5 groups of Chinese medicine treatment (P<0.01), and no significant difference between high dose group of Juqi Maxingshigan decoction and negative control group (P>0.05). Taken together, both of the two prescriptions have certain effects on mycoplasma pneumonia of goats, and the medium dose group of Yinqin Maxingshigan decoction has better effect.

Key words: mycoplasmal pneumonia of goats, Yinqin Maxingshigan decoction, Juqi Maxingshigan decoction, cytokines, tissue observation

CLC Number:

S858.26

XIE Huimei, ZHANG Junsheng, GUO Guangfu, DING Xiaoli, GAO Deli, SUN Shengjun. Study on Anti- Mycoplasma capricolumsubsp capricolum by Yinqin Maxingshigan Decoction and Juqi Maxingshigan Decoction[J]. Chinese Agricultural Science Bulletin, 2022, 38(35): 97-104.

Figures/Tables 17

References 29

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组别	0 d	7 d	14 d	21 d
阴性对照组	325.09±20.15^a	327.79±17.32^A	320.36±13.82^aA	320.44±13.73^A
阳性对照组	324.65±18.11^a	355.20±12.45^AB	380.85±15.49^dC	404.10±18.80^C
银芩低剂量	326.12±16.52^a	351.13±12.99 ^AB	351.13±12.99^cB	346.50±12.73^B
银芩中剂量	327.09±18.21^a	352.23±15.52 ^AB	334.82±11.22^bB	326.46±14.12^A
银芩高剂量	326.98±19.01^a	350.43±15.92 ^AB	355.79±13.62^cB	346.79±20.05^B
桔芪低剂量	325.66±16.68^a	351.85±15.72 ^AB	356.41±12.94^cB	357.42±15.45^B
桔芪中剂量	326.61±17.88^a	350.72±10.81 ^AB	351.35±11.64^cB	352.84±14.97^B
桔芪高剂量	327.31±18.32^a	350.19±11.98 ^AB	350.96±17.05^cB	347.56±15.26^B

组别	0 d	7 d	14 d	21 d
阴性对照组	325.09±20.15^a	327.79±17.32^A	320.36±13.82^aA	320.44±13.73^A
阳性对照组	324.65±18.11^a	355.20±12.45^AB	380.85±15.49^dC	404.10±18.80^C
银芩低剂量	326.12±16.52^a	351.13±12.99 ^AB	351.13±12.99^cB	346.50±12.73^B
银芩中剂量	327.09±18.21^a	352.23±15.52 ^AB	334.82±11.22^bB	326.46±14.12^A
银芩高剂量	326.98±19.01^a	350.43±15.92 ^AB	355.79±13.62^cB	346.79±20.05^B
桔芪低剂量	325.66±16.68^a	351.85±15.72 ^AB	356.41±12.94^cB	357.42±15.45^B
桔芪中剂量	326.61±17.88^a	350.72±10.81 ^AB	351.35±11.64^cB	352.84±14.97^B
桔芪高剂量	327.31±18.32^a	350.19±11.98 ^AB	350.96±17.05^cB	347.56±15.26^B

组别	0 d	7 d	14 d	21 d
阴性对照组	45.97±11.25^a	45.45±10.52^A	46.55±11.07^aA	46.50±11.11^A
阳性对照组	43.69±12.08^a	58.01±10.95^B	86.63±11.70^dC	99.76±10.01^D
银芩低剂量	45.76±11.09^a	58.02±11.42^B	61.19±11.72^cB	64.29±10.20C
银芩中剂量	44.67±12.12^a	56.57±10.17^B	55.05±11.18^bB	53.21±11.60^B
银芩高剂量	45.87±11.55^a	57.94±10.33^B	59.51±8.47^cB	63.15±11.09^C
桔芪低剂量	43.95±11.65^a	57.22±11.16^B	61.97±11.38^cB	62.92±11.83^C
桔芪中剂量	44.78±10.98^a	57.80±11.21^B	61.39±11.23^cB	67.63±10.94^C
桔芪高剂量	45.56±12.05^a	57.05±11.31^B	63.24±11.45^cB	58.26±10.51^C

组别	0 d	7 d	14 d	21 d
阴性对照组	45.97±11.25^a	45.45±10.52^A	46.55±11.07^aA	46.50±11.11^A
阳性对照组	43.69±12.08^a	58.01±10.95^B	86.63±11.70^dC	99.76±10.01^D
银芩低剂量	45.76±11.09^a	58.02±11.42^B	61.19±11.72^cB	64.29±10.20C
银芩中剂量	44.67±12.12^a	56.57±10.17^B	55.05±11.18^bB	53.21±11.60^B
银芩高剂量	45.87±11.55^a	57.94±10.33^B	59.51±8.47^cB	63.15±11.09^C
桔芪低剂量	43.95±11.65^a	57.22±11.16^B	61.97±11.38^cB	62.92±11.83^C
桔芪中剂量	44.78±10.98^a	57.80±11.21^B	61.39±11.23^cB	67.63±10.94^C
桔芪高剂量	45.56±12.05^a	57.05±11.31^B	63.24±11.45^cB	58.26±10.51^C

组别	0 d	7 d	14 d	21 d
阴性对照组	163.76±28.15^a	165.26±23.13^A	168.49±26.62^aA	169.02±29.09^A
阳性对照组	162.98±21.98^a	196.95±24.79^B	231.68±24.56^dC	223.50±23.55^C
银芩低剂量	164.56±22.76^a	197.20±25.29^B	209.52±23.53^cB	195.62±28.62^B
银芩中剂量	163.78±25.64^a	196.71±24.17^B	210.85±24.58^cB	189.46±23.25^B
银芩高剂量	165.12±24.09^a	196.81±24.16^B	204.69±28.98^cB	186.90±24.42^B
桔芪低剂量	163.89±22.33^a	196.96±28.66^B	211.92±23.71^cB	194.06±26.53^B
桔芪中剂量	164.65±26.23^a	197.12±23.86^B	210.11±22.14^cB	191.62±28.91^B
桔芪高剂量	163.88±24.69^a	196.28±27.06^B	185.71±30.06^bB	176.46±22.32^A