The Immunomodulatory Effect of Auricularia curicula Lectin on RAW264.7 Cells

doi:10.11924/j.issn.1000-6850.casb2020-0228

Abstract

Abstract:

The paper aims to solve the problem of the release of IL-6, IL-1β, TNF-α, NO and the immune regulation effect of Auricularia auricula lectin on RAW246.7 cells. The A. auricula lectin was separated and purified by 40% (NH₄)₂SO₄precipitation method, and accompanied by the detection of coagulation activity and amino acid composition, the crude product of A. auricula lectin was obtained. Through the AKTA protein purification system, pure A. auricula lectin was obtained. The molecular weight of the lectin was analyzed by using SDS-PAGE electrophoresis. The Griess method was used to detect the secretion of NO by macrophage RAW264.7. ELISA was used to detect the release of IL-6, IL-1β and TNF-α. The results showed that the molecular weight of the A. auricula lectin was about 20 kDa. Through detection, it was found that A. auricula lectin could promote the secretion of cytokines IL-6, IL-1β and TNF-α by macrophage RAW264.7, and it showed a dose dependent and immunomodulatory effect.

Key words: Auricularia auricula, lectin, extraction and purification, immune regulation, cytokines

CLC Number:

S646.6

Fang Jiamin, Ma Chengyao, Zhang Yanlong. The Immunomodulatory Effect of Auricularia curicula Lectin on RAW264.7 Cells[J]. Chinese Agricultural Science Bulletin, 2021, 37(9): 125-130.

Figures/Tables 7

References 24

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氨基酸种类	氨基酸含量/mg	氨基酸种类	氨基酸含量/mg
天冬氨酸	11.83	异亮氨酸	10.70
苏氨酸	5.67	亮氨酸	10.63
丝氨酸	4.79	酪氨酸	7.17
谷氨酸	13.46	苯丙氨酸	6.67
半胱氨酸	10.31	赖氨酸	2.03
丙氨酸	7.45	组氨酸	3.14
蛋氨酸	3.73	精氨酸	2.42

氨基酸种类	氨基酸含量/mg	氨基酸种类	氨基酸含量/mg
天冬氨酸	11.83	异亮氨酸	10.70
苏氨酸	5.67	亮氨酸	10.63
丝氨酸	4.79	酪氨酸	7.17
谷氨酸	13.46	苯丙氨酸	6.67
半胱氨酸	10.31	赖氨酸	2.03
丙氨酸	7.45	组氨酸	3.14
蛋氨酸	3.73	精氨酸	2.42