Effects of Polygonum cuspidatum on Liver Enzyme Activity of Red Tilapia Injected with Streptococcus agalactiae

doi:10.11924/j.issn.1000-6850.casb2022-0163

Abstract

Abstract:

In order to explore the effect of Polygonum cuspidatum crude extract and resveratrol on the liver enzyme activity of red tilapia injected with Streptococcus agalactiae, the red tilapia (Oreochromis mossambicus×O. niloticus) were randomly divided into 24 groups, 12 groups were fed normal diet (no drug addition group), and other 12 groups were fed diet supplemented with P. cuspidatum crude extract and resveratrol (drug addition group) in this study. Three groups of fish were selected from the drug addition group and the no drug addition group to inject S. agalactiae bacterial solution at three concentration gradients respectively. At the same time, the control group was set up without injection of bacterial solution, and all groups were continuously observed for 21 days, and the number of deaths was recorded. The activity of superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), acid phosphatase (ACP), alkaline phosphatase (AKP) and lysozyme (LZM) in the liver of red tilapia were tested after 21 days. The result showed that P. cuspidatum crude extract and resveratrol reduced the body damage of S. agalactiae by enhancing the antioxidant ability of red tilapia in the low concentration (7×10³ CFU/mL) of S. agalactiae groups. When fish were exposed to high concentration (7×10⁵ CFU/mL) of S. agalactiae, P. cuspidatum crude extract and resveratrol increased the resistance of fish by inducing the activity of phosphatase in the liver. At the same time, the crude extract of P. cuspidatum and resveratrol kept the mortality rate of red tilapia injected with S. agalactiae unchanged after 12 days. This experiment showed that P. cuspidatum crude extract and resveratrol could reduce the damage of S. agalactiae to fish and control the mortality under high and low concentrations of S. agalactiae stress.

Key words: red tilapia, Streptococcus agalactiae, crude extract from Polygonum cuspidatum, resveratrol

JING Xiaojun, KONG Xinru, HUA Haokun, LIU Tingyan, AN Shuwei, GU Lingling. Effects of Polygonum cuspidatum on Liver Enzyme Activity of Red Tilapia Injected with Streptococcus agalactiae[J]. Chinese Agricultural Science Bulletin, 2023, 39(18): 151-157.

Figures/Tables 4

References 30

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