Lethal Effect of Spinosad Combined with Mung Bean Water Content Control on Callosobruchus chinensis

doi:10.11924/j.issn.1000-6850.casb2022-0428

Abstract

Abstract:

In order to reduce the dosage of spinosad and delay the generation of drug resistance, the lethal effect of spinosad on Callosobruchus chinensis was tested by being combined with water content control of mung bean. The seed dressing method was used to determine the lethal effect of spinosad on adults and eggs of C. chinensis. The results showed that the mortality of the eggs and adults increased with the increase of spinosad concentration and the decrease of mung bean water content. The eggs of C. chinensis were more sensitive to spinosad, the mortality of eggs treated with adult sublethal LC₁₀ concentration was as high as 65%. It is recommended to control the water content of mung bean in the range of 9.5%-11.5% when using spinosad to control C. chinensis. This study can provide reference for the environment-friendly management of mung bean storage.

Key words: spinosad, water content, mung bean, Callosobruchus chinensis, lethal effect

CHENG Zhiyong, WANG Qi, ZHOU Hang, WU Meihui, ZHANG Lixiang. Lethal Effect of Spinosad Combined with Mung Bean Water Content Control on Callosobruchus chinensis[J]. Chinese Agricultural Science Bulletin, 2023, 39(14): 115-118.

Figures/Tables 4

References 18

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多杀霉素浓度/(mL/kg)	含水量7.5%	含水量9.5%	含水量11.5%	含水量13.5%
15	(75.56±4.44) ^a	(58.52±5.96) ^a	(53.33+3.33)^a	(46.67+3.33)^a
12.5	(58.15±7.44)^ab	(48.52±4.55)^ab	(40.00±5.77)^b	(36.67±6.67)^a
10	(54.81±7.76)^bc	(37.41±8.12)^bc	(30.00±0.00)^c	(23.33±3.33)^b
7.5	(37.78±6.19)^cd	(24.07±3.03)^cd	(26.67±3.33)^cd	(13.33±3.33)^bc
5	(17.04±6.49)^ef	(17.04±2.96)^de	(23.33±3.33)^cde	(13.33±3.33)^bc
2.5	(23.70±6.30)^de	(17.04±2.96)^de	(13.33±3.33)^e	(13.33±3.33)^bc
1	(23.70±6.30)^de	(20.37±5.46)^d	(16.67±3.33)^de	(10.00±0.00)^cd
CK	(3.33±3.33)^f	(3.33±3.33)^e	(0.00±0.00)^f	(0.00±0.00)^d

多杀霉素浓度/(mL/kg)	含水量7.5%	含水量9.5%	含水量11.5%	含水量13.5%
15	(75.56±4.44) ^a	(58.52±5.96) ^a	(53.33+3.33)^a	(46.67+3.33)^a
12.5	(58.15±7.44)^ab	(48.52±4.55)^ab	(40.00±5.77)^b	(36.67±6.67)^a
10	(54.81±7.76)^bc	(37.41±8.12)^bc	(30.00±0.00)^c	(23.33±3.33)^b
7.5	(37.78±6.19)^cd	(24.07±3.03)^cd	(26.67±3.33)^cd	(13.33±3.33)^bc
5	(17.04±6.49)^ef	(17.04±2.96)^de	(23.33±3.33)^cde	(13.33±3.33)^bc
2.5	(23.70±6.30)^de	(17.04±2.96)^de	(13.33±3.33)^e	(13.33±3.33)^bc
1	(23.70±6.30)^de	(20.37±5.46)^d	(16.67±3.33)^de	(10.00±0.00)^cd
CK	(3.33±3.33)^f	(3.33±3.33)^e	(0.00±0.00)^f	(0.00±0.00)^d

含水量/%	1	2.5	5	7.5	10	12.5	15
13.5	(10.00±0.00)^a	(13.33±3.33)^a	(13.33±3.33)^a	(13.33±3.33)^a	(23.33±3.33)^a	(36.67±6.67)^a	(46.67+3.33)^a
11.5	(16.67±3.33)^a	(13.33±3.33)^a	(23.33±3.33)^a	(26.67±3.33)^ab	(30.00±0.00)^a	(40.00±5.77)^a	(53.33+3.33)^a
9.5	(20.37±5.46)^a	(17.04±2.96)^a	(17.04±2.96)^a	(24.07±3.03)^ab	(37.41±8.12)^ab	(48.52±4.55)^a	(58.52±5.96) ^a
7.5	(23.70±6.30)^a	(23.70±6.30)^a	(17.04±6.49)^a	(37.78±6.19)^b	(54.81±7.76)^b	(58.15±7.44)^a	(75.56±4.44) ^b

含水量/%	1	2.5	5	7.5	10	12.5	15
13.5	(10.00±0.00)^a	(13.33±3.33)^a	(13.33±3.33)^a	(13.33±3.33)^a	(23.33±3.33)^a	(36.67±6.67)^a	(46.67+3.33)^a
11.5	(16.67±3.33)^a	(13.33±3.33)^a	(23.33±3.33)^a	(26.67±3.33)^ab	(30.00±0.00)^a	(40.00±5.77)^a	(53.33+3.33)^a
9.5	(20.37±5.46)^a	(17.04±2.96)^a	(17.04±2.96)^a	(24.07±3.03)^ab	(37.41±8.12)^ab	(48.52±4.55)^a	(58.52±5.96) ^a
7.5	(23.70±6.30)^a	(23.70±6.30)^a	(17.04±6.49)^a	(37.78±6.19)^b	(54.81±7.76)^b	(58.15±7.44)^a	(75.56±4.44) ^b

含水量/%	回归方程	相关系数r	卡方值X²	LC₅₀/(mL/kg)
7.5	Y=0.112+0.04x	0.856	9.952	9.700
9.5	Y=0.089+0.03x	0.850	6.048	13.700
11.5	Y=0.094+0.026x	0.893	9.000	15.615
13.5	Y=0.032+0.025x	0.854	8.762	18.720