Regulating Effect of Several Plant Growth Regulators on Seed Germination and Seedling Quality of Rice

doi:10.11924/j.issn.1000-6850.casb2022-0625

Abstract

Abstract:

In order to cultivate healthy rice seedlings and improve the quality of rice seedlings, a soaking seeds experiment which used ‘Jinyujing 22’ as experimental material, was conducted to assess the effect of four plant growth regulators on seed germination and seedling quality under different concentrations and soaking times. The plant growth regulators were respectively 5% indole butyric acid·naphthalene acetic acid solution (A), 1% indole butyric acid·abscisic acid solution (B), 0.25% abscisic acid aqueous solution (C), 0.136% gibberellic acid·indole acetic acid·brassinolide wettable powder (D). The results showed that the germination potential was increased after soaking seeds with 20 mg/kg A for 24 h and 0.4 mg/kg C for 24 h and 48 h. Different treatments in this study had little effect on germination index and germination rate of rice seeds. The seedling index of rice seedlings was significantly increased by 19.14%, 17.68%, 34.55% and 23.80% when treated with A at the concentration of 20 mg/kg, B at the concentration of 10 mg/kg, C at the concentration of 0.4 mg/kg and D at the concentration of 1000 times after soaking at 25℃ for 24 h, respectively. In conclusion, the optimum method in the agricultural production for increasing seed emergence rate and seeding index was that rice seed were soaked with 20 mg/kg of 5% indole butyric acid·naphthalene acetic acid solution, 10 mg/kg of 1% indole butyric acid·abscisic acid solution, 0.4 mg/kg of 0.25% abscisic acid aqueous solution or 1000 times of 0.136% gibberellic acid·indole acetic acid·brassinolide wettable powder for 24 h.

Key words: rice, plant growth regulator, soaking seeds, quality of rice seedlings, seeding index

SUN Shuqin, LI Yuejiao, LI Guangsheng, YANG Xiurong. Regulating Effect of Several Plant Growth Regulators on Seed Germination and Seedling Quality of Rice[J]. Chinese Agricultural Science Bulletin, 2023, 39(33): 1-7.

Figures/Tables 7

References 28

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试验药剂	试验浓度
5%吲哚丁酸·萘乙酸可溶液剂	20 mg/kg	40 mg/kg	60 mg/kg
1%吲哚丁酸·诱抗素可溶液剂	10 mg/kg	20 mg/kg	30 mg/kg
0.25%S-诱抗素水剂	0.3 mg/kg	0.4 mg/kg	0.5 mg/kg
0.136%赤·吲乙·芸苔可湿性粉剂	2000倍液	1000倍液	500倍液
清水	—	—	—

试验药剂	试验浓度
5%吲哚丁酸·萘乙酸可溶液剂	20 mg/kg	40 mg/kg	60 mg/kg
1%吲哚丁酸·诱抗素可溶液剂	10 mg/kg	20 mg/kg	30 mg/kg
0.25%S-诱抗素水剂	0.3 mg/kg	0.4 mg/kg	0.5 mg/kg
0.136%赤·吲乙·芸苔可湿性粉剂	2000倍液	1000倍液	500倍液
清水	—	—	—

试验药剂	试验浓度	出苗率/%	株高/cm	根长/cm	茎粗/mm
5%吲哚丁酸·萘乙酸可溶液剂	20 mg/kg	88±3.06	13.31±0.24^**	10.62±0.35	2.14±0.02^**
	40 mg/kg	65.33±1.76^**	12.56±0.27	9.86±0.42	2.11±0.03^**
	60 mg/kg	52±3.06^**	11.31±0.09 ^**	9.02±0.22	2.12±0.06^**
1%吲哚丁酸·诱抗素可溶液剂	10 mg/kg	92.67±3.53	14.85±0.23 ^**	10.9±0.39	2.14±0.03^**
	20 mg/kg	80±2.31	13.42±0.35 ^**	9.89±0.24	2±0.03
	30 mg/kg	61.33±2.91 ^**	12.32±0.24	9.28±0.26	1.96±0.05
0.25%S-诱抗素水剂	0.3 mg/kg	84.33±1.2	12.9±0.01	9.79±0.15	1.98±0.05
	0.4 mg/kg	92±1.15	12.62±0.08	11.02±0.25^*	1.95±0.03
	0.5 mg/kg	72.67±3.71 ^**	10.11±0.04 ^**	9.95±0.2	1.92±0.02
0.136%赤·吲乙·芸苔可湿性粉剂	2000倍液	90.67±1.76	13.46±0.13^**	10.83±0.55	2.1±0.03^**
	1000倍液	91.33±0.67	13.52±0.06^**	11.08±0.55^*	2.12±0.09^**
	500倍液	80.67±4.81	13.17±0.11^*	11.35±0.55^**	2.24±0.02^**
清水	—	84±3.06	12.38±0.28	9.77±0.27	1.88±0.08

试验药剂	试验浓度	出苗率/%	株高/cm	根长/cm	茎粗/mm
5%吲哚丁酸·萘乙酸可溶液剂	20 mg/kg	88±3.06	13.31±0.24^**	10.62±0.35	2.14±0.02^**
	40 mg/kg	65.33±1.76^**	12.56±0.27	9.86±0.42	2.11±0.03^**
	60 mg/kg	52±3.06^**	11.31±0.09 ^**	9.02±0.22	2.12±0.06^**
1%吲哚丁酸·诱抗素可溶液剂	10 mg/kg	92.67±3.53	14.85±0.23 ^**	10.9±0.39	2.14±0.03^**
	20 mg/kg	80±2.31	13.42±0.35 ^**	9.89±0.24	2±0.03
	30 mg/kg	61.33±2.91 ^**	12.32±0.24	9.28±0.26	1.96±0.05
0.25%S-诱抗素水剂	0.3 mg/kg	84.33±1.2	12.9±0.01	9.79±0.15	1.98±0.05
	0.4 mg/kg	92±1.15	12.62±0.08	11.02±0.25^*	1.95±0.03
	0.5 mg/kg	72.67±3.71 ^**	10.11±0.04 ^**	9.95±0.2	1.92±0.02
0.136%赤·吲乙·芸苔可湿性粉剂	2000倍液	90.67±1.76	13.46±0.13^**	10.83±0.55	2.1±0.03^**
	1000倍液	91.33±0.67	13.52±0.06^**	11.08±0.55^*	2.12±0.09^**
	500倍液	80.67±4.81	13.17±0.11^*	11.35±0.55^**	2.24±0.02^**
清水	—	84±3.06	12.38±0.28	9.77±0.27	1.88±0.08

试验药剂	试验浓度	20株地上干重/g	20株地下干重/g	壮苗指数	壮苗指数增加率/%		根冠比
5%吲哚丁酸·萘乙酸可溶液剂	20 mg/kg	0.3757±0.005^**	0.2880±0.004 ^**	0.0260±0^**	19.14		0.7666±0.003^**
	40 mg/kg	0.4043±0.01^**	0.2807±0.006 ^*	0.0244±0.001^**	11.71		0.6944±0.008^**
	60 mg/kg	0.4013±0.014^**	0.2653±0.005	0.0227±0	3.98		0.662±0.012^**
1%吲哚丁酸·诱抗素可溶液剂	10 mg/kg	0.4560±0.006	0.3020±0.005^**	0.0257±0.001^**	17.68		0.6624±0.011^**
	20 mg/kg	0.4620±0.007	0.3040±0.004^**	0.0258±0^**	18.27		0.6584±0.014^**
	30 mg/kg	0.4600±0.003	0.2967±0.003^**	0.0250±0^**	14.68		0.6449±0.003^**
0.25%S-诱抗素水剂	0.3 mg/kg	0.3963±0.007^**	0.2710±0.007	0.0233±0 ^*	7.01		0.6838±0.002^**
	0.4 mg/kg	0.409±0.005^**	0.3287±0.005^**	0.0293±0.001^**	34.55		0.8037±0.012^**
	0.5mg/kg	0.341±0.002^**	0.311±0.006^**	0.0297±0.001^**	36.04		0.9119±0.013^**
0.136%赤·吲乙·芸苔可湿性粉剂	2000倍液	0.4130±0.007^**	0.2783±0.006	0.0238±0.001^*	9.37	0.6741±0.014^**
	1000倍液	0.4293±0.004	0.3077±0.006 ^**	0.0270±0.001 ^**	23.80	0.7165±0.009 ^**
	500倍液	0.4527±0.008	0.3193±0.008 ^**	0.0279±0 ^**	27.95	0.7059±0.012 ^**
清水	—	0.4423±0.003	0.2653±0.003	0.0218±0	—	0.5998±0.008