Analysis of Chemical Composition and Polyphenol Oxidase of Different Amorphophallus Bulbifer Varieties

doi:10.11924/j.issn.1000-6850.casb2022-0935

Abstract

Abstract:

In order to study the chemical composition and polyphenol oxidase (PPO) characteristics of different amorphophallus bulbifer varieties, the glucomannan, crude fiber, protein, alkaloid and heavy metal elements of five amorphophallus bulbifer varieties were determined and the optimum temperature, pH and thermal stability of PPO for three amorphophallus bulbifer varieties were studied. The results showed that glucomannan content of five amorphophallus bulbifer varieties ranged from 55.30% to 63.90%, crude fiber from 2.77% to 3.47%, protein from 8.08% to 10.50%, alkaloids from 1.73% to 1.89%, manganese from 4.36 to 12.50 mg/kg, cadmium from 0.0154 to 0.206 mg/kg, arsenic from 0 to 0.0102 mg/kg, and there were significant differences (P < 0.05); fat content ranged from 0.27% to 0.35% with no significant difference (P > 0.05). The optimum temperatures of PPO for ‘A. muelleri’, ‘XiDai 9’and ‘YunRe 1701’were 35 ℃, 40 ℃ and 35 ℃, and the optimum pH were 6.0, 6.0 and 5.5. When the temperature reached 90 ℃, the PPO would be inactivated in a short time. There were differences in chemical composition and PPO of different varieties, and this study can provide some reference for the screening and processing of amorphophallus bulbifer varieties.

Key words: amorphophallus bulbifer, chemical composition, heavy metal, polyphenol oxidase, variety comparison

FU Jiarong, XU Rong, JIANG Shikuan, TIAN Yaohua, MA Shangxuan, YAN Li, ZOU Jianyun, GUO Gangjun. Analysis of Chemical Composition and Polyphenol Oxidase of Different Amorphophallus Bulbifer Varieties[J]. Chinese Agricultural Science Bulletin, 2023, 39(30): 158-164.

Figures/Tables 5

References 26

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品种	葡甘聚糖	粗脂肪	粗纤维	蛋白质	生物碱
弥勒魔芋	59.71±0.84^b	0.31±0.01^a	3.47±0.16^a	10.20±0.15^b	1.80±0.08^ab
西傣9号	55.30±0.46^c	0.28±0.02^a	2.83±0.10^c	8.08±0.02^d	1.74±0.11^b
云热1701	63.90±0.56^a	0.28±0.02^a	3.25±0.04^b	10.50±0.06^a	1.85±0.04^ab
云热1702	63.01±0.54^a	0.34±0.10^a	2.77±0.08^c	9.96±0.01^c	1.89±0.08^a
云热1709	62.88±0.98^a	0.27±0.05^a	2.93±0.02^b	8.08±0.02^d	1.73±0.03^b

品种	葡甘聚糖	粗脂肪	粗纤维	蛋白质	生物碱
弥勒魔芋	59.71±0.84^b	0.31±0.01^a	3.47±0.16^a	10.20±0.15^b	1.80±0.08^ab
西傣9号	55.30±0.46^c	0.28±0.02^a	2.83±0.10^c	8.08±0.02^d	1.74±0.11^b
云热1701	63.90±0.56^a	0.28±0.02^a	3.25±0.04^b	10.50±0.06^a	1.85±0.04^ab
云热1702	63.01±0.54^a	0.34±0.10^a	2.77±0.08^c	9.96±0.01^c	1.89±0.08^a
云热1709	62.88±0.98^a	0.27±0.05^a	2.93±0.02^b	8.08±0.02^d	1.73±0.03^b

元素	弥勒魔芋	西傣9号	云热1701	云热1702	云热1709
锰	6.72±0.09^d	4.36±0.09^e	12.50±0.36^a	10.4±0.17^c	11.5±0.13^b
铅	—	—	—	—	—
汞	—	—	—	—	—
镉	0.0154±0.0006^d	0.0829±0.0008^b	0.0783±0.0009^b	0.0398±0.0009^c	0.206±0.008^a
砷	0.0071±0.0001^b	—	0.0102±0.0007^a	—	0.0065±0.0001^c
铬	—	—	—	—	—
镍	—	—	—	—	—

元素	弥勒魔芋	西傣9号	云热1701	云热1702	云热1709
锰	6.72±0.09^d	4.36±0.09^e	12.50±0.36^a	10.4±0.17^c	11.5±0.13^b
铅	—	—	—	—	—
汞	—	—	—	—	—
镉	0.0154±0.0006^d	0.0829±0.0008^b	0.0783±0.0009^b	0.0398±0.0009^c	0.206±0.008^a
砷	0.0071±0.0001^b	—	0.0102±0.0007^a	—	0.0065±0.0001^c
铬	—	—	—	—	—
镍	—	—	—	—	—

保温时间/min	70℃保温PPO相对活力/%			80℃保温PPO相对活力/%
保温时间/min	弥勒魔芋	云热1701	西傣9号	弥勒魔芋	云热1701	西傣9号
0	100.00±0.00	100.00±0.00	100.00±0.00	100.00±0.00	100.00±0.00	100.00±0.00
5	77.35±1.06	68.41±0.00	57.93±0.00	56.98±0.07	39.82±0.00	43.06±0.00
10	71.29±0.08	54.33±0.72	52.29±0.53	57.75±0.85	35.89±0.36	35.19±0.53
15	70.75±0.91	53.22±2.15	51.51±0.65	53.25±0.71	17.62±1.47	22.87±0.80
20	61.90±0.91	43.58±0.36	42.20±0.66	43.77±0.49	16.33±0.79	18.33±1.09
25	68.57±1.60	42.35±0.98	40.50±1.05	43.70±1.27	15.17±0.51	13.06±1.57
30	57.75±0.85	35.43±1.23	38.92±0.14	21.57±0.91	10.07±0.22	10.97±1.63
保温时间/min	90℃保温PPO相对活力/%			100℃保温PPO相对活力/%
保温时间/min	弥勒魔芋	云热1701	西傣9号	弥勒魔芋	云热1701	西傣9号
0	100.00±0.00	100.00±0.00	100.00±0.00	100.00±0.00	100.00±0.00	100.00±0.00
5	1.66±0.08	6.91±0.00	9.31±0.00	0.28±0.00	0.43±0.00	0.65±0.00
10	0.28±0.00	0.43±0.15	5.28±0.13	0.43±0.00	0.43±0.00	0.56±0.00
15	0.36±0.21	0.43±0.14	2.50±0.27	0.43±0.00	0.48±0.00	0.65±0.00
20	0.43±0.00	0.44±0.00	1.11±0.00	0.57±0.00	0.53±0.00	0.56±0.00
25	0.43±0.07	0.38±0.22	0.56±0.26	0.19±0.00	0.14±0.00	0.56±0.00
30	0.57±0.00	0.40±0.51	0.56±0.26	0.00±0.00	0.00±0.00	0.00±0.00