Growth Promoting Characteristics of Rhizosphere Growth Promoting Bacteria of Codonopsis pilosula and Effects of Its Volatile Substances on Crop Growth

doi:10.11924/j.issn.1000-6850.casb2022-0959

Abstract

Abstract:

The growth-promoting characteristics of plant growth-promoting rhizobacteria (PGPR) strain DM11 and the effects of volatile compounds (VOCs) produced by DM11 on plant growth were investigated. DM11 was identified by morphological, physiological and biochemical indexes, BIOLOG system and 16s rDNA sequence. The VOCs were analyzed by headspace gas chromatography-mass spectrometry (HS-GC-MS). The antifungal activity and growth-promoting effects on cucumber and tomato of VOCs produced by strain DM11 were studied by the dual culture on two-section of a Petri dish. The growth-promoting characteristics of the strain and its growth-promoting effect on seedlings were determined. DM11 was identified as Pseudomonas thivervalensis, which was shown to produce IAA, siderophore, solubilize insoluble phosphate, fix nitrogen and tolerate salt stress. The VOCs produced by strain DM11 could promote the growth of cucumber and tomato and the indexes related to induced systemic resistance (ISR). There were 4 kinds of VOCs, and the main component 1-undecene could promote the growth of two crops and inhibit the growth of three pathogenic fungi..DM11 can promote the growth of cucumber and tomato, and the number of leaves and seedling height increased by 15.94%, 11.63%, 7.50% and 6.55%, respectively. DM11 strain and its VOCs have the potential to be developed as biological agents.

Key words: plant growth-promoting rhizobacteria (PGPR), volatile organic compounds (VOCs), promoting growth, induced systemic resistance (ISR), Pseudomonas thivervalensis

MENG Jing, ZHANG Lihui, BAI Bianxia, REN Jiahong. Growth Promoting Characteristics of Rhizosphere Growth Promoting Bacteria of Codonopsis pilosula and Effects of Its Volatile Substances on Crop Growth[J]. Chinese Agricultural Science Bulletin, 2023, 39(30): 123-131.

Figures/Tables 14

References 31

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农作物	处理	主根长	芽长	侧根数
黄瓜	CK	1.560±0.053	4.109±0.487	9.467±1.642
黄瓜	DM11	2.799±0.454	5.255±0.397	14.067±1.486
番茄	CK	1.829±0.134	4.109±0.487
番茄	DM11	2.595±0.212	5.171±0.609

农作物	处理	主根长	芽长	侧根数
黄瓜	CK	1.560±0.053	4.109±0.487	9.467±1.642
黄瓜	DM11	2.799±0.454	5.255±0.397	14.067±1.486
番茄	CK	1.829±0.134	4.109±0.487
番茄	DM11	2.595±0.212	5.171±0.609

保留时间/min	面积/%	名称
8.945	0.164	苯乙酮(Acetophenone)
9.397	44.361	1-十一烯(1-Undecene)
11.416	0.703	1H-Indene,1-methylene-
22.210	0.551	2,5-二叔丁基酚(Phenol,2,5-bis(1,1-dimethylethyl)-)

保留时间/min	面积/%	名称
8.945	0.164	苯乙酮(Acetophenone)
9.397	44.361	1-十一烯(1-Undecene)
11.416	0.703	1H-Indene,1-methylene-
22.210	0.551	2,5-二叔丁基酚(Phenol,2,5-bis(1,1-dimethylethyl)-)

病原菌	80 µL	100 μL	120 μL	140 μL
香蕉枯萎病菌	4.95±1.93d	23.22±0.54c	76.16±1.42b	94.74±3.26a
尖孢镰刀菌	35.33±1.97d	86.44±0.55c	94.01±1.97b	97.16±0.95a
棉花立枯菌	19.47±2.06d	58.09±2.06c	87.79±2.49b	97.03±2.97a