Effects on Growth, Photosynthetic Parameters, Physiology and Biochemistry of Bean Seedlings: CaCl2 Initiation

doi:10.11924/j.issn.1000-6850.casb2023-0344

Abstract

Abstract:

The aim was to study the effects of CaCl₂ initiation on bean seedlings, and to provide practical reference value for promoting the growth and the physiological and biochemical effects produced by CaCl₂ seedlings initiation. The bean variety ‘Jiangjun’ was used as the experimental material, and 40 mmol/L CaCl₂ was used to initiate the bean seeds for 12 h. The results showed that 12 h after CaCl₂ induction, the dry weight of beans seedlings was increased by 38.8% and 19.2% compared with dry seed control (CK₁) and distilled water control (CK₂), respectively. The net photosynthetic rates were increased by 25.8% and 16.4%, respectively. The total activity of (α+β) amylase was increased by 79.9% and 15.3%, respectively. SOD, POD and CAT showed an increasing trend compared with the two controls, and CAT was the most significant, which was increased by 3.5% and 13.5% compared with CK₁ and CK₂, respectively. The soluble sugar content was not significantly different from CK₂, but it was 106.6% higher than CK₁. Soluble protein was increased by 16.4% and 3.9%, respectively. Compared with CK₁ and CK₂, MDA was decreased by 39.0% and 11.8%, and O₂^- was decreased by 15.5% and 12.1%, respectively. After initiation of 40 mmol/L CaCl₂ for 12 h, the photosynthetic efficiency was effectively improved, and various physiological and biochemical indexes were improved, thus it promoted the growth of bean.

Key words: common bean, seed, initiator, photosynthetic parameters, physiological and biochemical index, CaCl₂

SHEN Qi, FENG Guojun, LIU Dajun, WEI Mengdan, LIU Chang, YANG Xiaoxu. Effects on Growth, Photosynthetic Parameters, Physiology and Biochemistry of Bean Seedlings: CaCl₂ Initiation[J]. Chinese Agricultural Science Bulletin, 2024, 40(24): 21-27.

Figures/Tables 11

References 28

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试管号	1	2	3	4	5	6	7
麦芽糖标准液/mL	0	0.2	0.6	1.0	1.4	1.8	2.0
蒸馏水/mL	2.0	1.8	1.4	1.0	0.6	0.2	0
麦芽糖含量/mg	0	0.2	0.6	1.0	1.4	1.8	2.0
3,5-二硝基水杨酸/mL	2.0	1.8	1.4	1.0	0.6	0.2	0

试管号	1	2	3	4	5	6	7
麦芽糖标准液/mL	0	0.2	0.6	1.0	1.4	1.8	2.0
蒸馏水/mL	2.0	1.8	1.4	1.0	0.6	0.2	0
麦芽糖含量/mg	0	0.2	0.6	1.0	1.4	1.8	2.0
3,5-二硝基水杨酸/mL	2.0	1.8	1.4	1.0	0.6	0.2	0

操作项目	α-淀粉酶活力				(α+β)-淀粉酶总活力
操作项目	Ⅰ-1	Ⅰ-2	Ⅰ-3		Ⅱ-4	Ⅱ-5	Ⅱ-6
淀粉酶原液/mL	1	1	1	0		0	0
钝化β-淀粉酶	70℃水浴15 min，流水冷却
淀粉酶稀释液/mL	0	0	0	1		1	1
3,5-二硝基水杨酸/mL	2	0	0	2		0	0
预保温	各试管与淀粉溶液在40℃恒温水浴中放置10 min
1%淀粉溶液/mL	1	1	1	1		1	1
保温	40℃恒温水浴5 min
3,5-二硝基水杨酸/mL	0	2	2	0		2	2

操作项目	α-淀粉酶活力				(α+β)-淀粉酶总活力
操作项目	Ⅰ-1	Ⅰ-2	Ⅰ-3		Ⅱ-4	Ⅱ-5	Ⅱ-6
淀粉酶原液/mL	1	1	1	0		0	0
钝化β-淀粉酶	70℃水浴15 min，流水冷却
淀粉酶稀释液/mL	0	0	0	1		1	1
3,5-二硝基水杨酸/mL	2	0	0	2		0	0
预保温	各试管与淀粉溶液在40℃恒温水浴中放置10 min
1%淀粉溶液/mL	1	1	1	1		1	1
保温	40℃恒温水浴5 min
3,5-二硝基水杨酸/mL	0	2	2	0		2	2

试管号	1	2	3	4	5	6
标准蛋白质/mL	0	0.2	0.4	0.6	0.8	1.0
蒸馏水量	1.0	0.8	0.6	0.4	0.2	0
蛋白质含量/μg	0	20	40	60	80	100

Effects on Growth, Photosynthetic Parameters, Physiology and Biochemistry of Bean Seedlings: CaCl₂ Initiation

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处理	蒸腾速率	净光合速率	叶片气孔导度	胞间CO₂浓度
CK₁	1.60	13.32	62.05	248.01
CK₂	1.64	14.39	73.73	266.41
C5	2.35	16.75	127.00	327.54

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