Abstract:

Apple scab caused by Venturia inaequalis Cook (Wint.) is one of the important apple diseases worldwide, which seriously affects the growth, yield, and fruit quality of apple trees. In order to establish a simple, fast, and sensitive molecular detection method for apple scab, a strain of apple scab (V. inaequalis) isolated and identified from the Yili River Valley in Xinjiang was used as the test material. Based on loop-mediated isothermal amplification (LAMP) technology, specific primers were designed and screened using the nucleic acid sequence of the ribosome transcription spacer (ITS) region of the pathogen as the target. A LAMP detection system for apple scab was established, and its specificity and sensitivity were verified. The results showed that among the 10 designed primers, the Venin7 primer could specifically identify apple scab from 4 apple diseases and 1 rust disease, and the detection stability was good; the LAMP detection system had good detection performance for apple scab at amplification temperatures of 55℃-70℃ and within 60-90 minutes; the sensitivity of detecting apple scab was 1 pg/μL, which was more than 10³ times higher than that of using specific primers for PCR detection. The LAMP detection technology established in this study can quickly and sensitively detect and monitor apple scab.

Key words: Yili River Valley, apple scab, loop-mediated isothermal amplification, pathogen detection, disease monitoring, control, ribosome transcription spacer (ITS) region, LAMP detection system