Clone and Functional Characterization of Strigolactone Receptor Gene in Arundo donax L.

doi:10.11924/j.issn.1000-6850.casb2024-0208

Abstract

Abstract:

In order to explore the molecular mechanism of the regulation of tiller in Arundo donax L., we cloned AdD14, a SL receptor gene in Arundo donax L., and conducted bioinformatics analysis and gene function verification. The SL receptor gene AdD14 (Genbank login number: OR915727) was homologous cloned from Arundo donax L. seedlings. ExPASy, MEGA and other software were used for bioinformatics analysis of the genes. The function of the gene was verified by the method of in vitro catalysis of the prokaryotic expression protein. The results showed that the AdD14 open reading frame was 816bp long and encoded 271 amino acid residues with a molecular weight of 29.75kDa. AdD14 protein was predicted to be an unstable hydrophobic protein with no transmembrane structure and no signal peptide. The phylogenetic tree showed that AdD14 had high homology with D14 protein of other species of plants, retained the conserved domain of SL receptor and catalytic triad, and could hydrolyze the fluorescent probe YLG (Yoshimulatone Green) of SL receptor, indicating that AdD14 was the SL receptor gene of Arundo donax L.. In this study, the AdD14 gene of Arundo donax L. was isolated, and its encoding protein was consistent with the structure and function of SL receptor D14 protein of other plants. It was initially confirmed that it was the SL receptor gene of Arundo donax L., which laid a certain foundation for further revealing the molecular mechanism of tiller regulation in Arundo donax L..

Key words: Arundo donax L., strigolactone receptor, gene clone, AdD14 gene, gene function verification, bioinformatics, tiller regulation

CHEN Yuchao, GUO Shenghu, TIAN Li, Zhong Nan, YANG Jianguo, GAN Xiaoyan. Clone and Functional Characterization of Strigolactone Receptor Gene in Arundo donax L.[J]. Chinese Agricultural Science Bulletin, 2025, 41(5): 50-55.

Figures/Tables 6

References 24

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