Cloning and Expression Analysis of EgNIP5;1 Gene in Oil Palm Under Boron Deficiency

doi:10.11924/j.issn.1000-6850.casb2020-0265

Abstract

Abstract:

This research aims to investigate the mechanism of boron transportation in oil palm and lay a theoretical foundation for the subsequent screening of boron efficient varieties. According to the sequence of AtNIP5;1 which is functional verified, we cloned the full-length sequence of boron transport channel protein gene EgNIP5;1. The gene structure, sequence characteristics, phylogenetic relationships, transmembrane domain and expression pattern were analyzed. The length of open reading frame of EgNIP5;1 was 894 bp, which coded a protein of 297 amino acids. EgNIP5;1 had highly conserved NPS, NPV and Ar/R motifs. EgNIP5;1 contained 4 extrons and 3 introns, and 5 transmembrane structures. Phylogenetic analysis showed that EgNIP5;1 was closely related to TcNIP5 of cacao. Real-time fluorescence quantitative PCR analysis showed that EgNIP5;1 was mainly expressed in roots of oil palm, and the expression ofEgNIP5;1 was induced by boron deficiency. The expression of EgNIP5;1 reached the maximum level at 48 h after boron deficiency treatment, which was 8.2 times of the control. This research suggests that EgNIP5;1 is possibly involved in the response to boron deficiency and plays an important role in boron absorption of oil palm.

Key words: oil palm, NIP5, gene clone, boron deficiency, gene expression, transmembrane structures

CLC Number:

S718.4

Jin Longfei, Yin Xinxing, Feng Meili, Zhou Lixia, Cao Hongxing. Cloning and Expression Analysis of EgNIP5;1 Gene in Oil Palm Under Boron Deficiency[J]. Chinese Agricultural Science Bulletin, 2021, 37(9): 22-27.

Figures/Tables 8

References 26

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引物功能	引物名称	引物序列	产物长度/bp
全长克隆	NIP5;1F	AGTTCCCCTTCCTAGCACCC	1182
全长克隆	NIP5;1R	TGTCACCACTCACAAAGCCA	1182
荧光定量	NIP5;1qF	CAGGCGTTCTTCCTCGAGTT	104
荧光定量	NIP5;1qR	ACTGCTATTCCGGCCAGTTC	104
内参基因	β-actin F	CTCAACCCCAAGGCGAAC	152
内参基因	β-actin R	GTAACACCATCTCCCGAGTCAA	152

引物功能	引物名称	引物序列	产物长度/bp
全长克隆	NIP5;1F	AGTTCCCCTTCCTAGCACCC	1182
全长克隆	NIP5;1R	TGTCACCACTCACAAAGCCA	1182
荧光定量	NIP5;1qF	CAGGCGTTCTTCCTCGAGTT	104
荧光定量	NIP5;1qR	ACTGCTATTCCGGCCAGTTC	104
内参基因	β-actin F	CTCAACCCCAAGGCGAAC	152
内参基因	β-actin R	GTAACACCATCTCCCGAGTCAA	152