Different Expression Levels of Virulence Genes of Bacterial Pathogen Associated with Acute Hepatopancreatic Necrosis Disease Under Different Environments

doi:10.11924/j.issn.1000-6850.casb20200300228

Abstract

Abstract:

Acute hepatopancreatic necrosis disease (AHPND) is caused by infection with Vibrio parahaemolyticus, and has cost huge economic losses to word shrimp culture industry. The aim is to study the differences in the expression of the main pathogenic factor Pir toxin gene under the treatment of different environmental factors, and to explore the possible measures to inhibit the expression of Pir toxin gene. In this study, the expression level of Pir toxin gene in pathogenic bacteria was detected by the application of fluorescence quantitative RT-PCR technology and the comparative CT value method under different environmental factors, such as temperature and pH. The results showed that the bacteria revealed positive amplification of the Pir toxin gene which was related to AHPND in a virulence plasmid harboring this strain. Under the condition of 18℃, 24℃, 30℃ and 36℃, the expression of Pir toxin gene increased with the rise of temperature, and under the condition of pH 7, pH 8, and pH 9, the expression level of Pir toxin gene decreased with the reduce of pH. Especially, the expression of Pir gene was remarkably influenced by the pH factor. The research results provide basic data for exploring the relationship between the pathogenicity of Vibrio parahaemolyticus and environmental factors, and also a scientific basis for the development of AHPND control in aquaculture.

Key words: Vibrio parahaemolyticus, AHPND, Pir gene, gene expression

CLC Number:

S917.1

An Wei, Xiao Yu, Gao Xiaohua, Peng Junhui, Tang Qian. Different Expression Levels of Virulence Genes of Bacterial Pathogen Associated with Acute Hepatopancreatic Necrosis Disease Under Different Environments[J]. Chinese Agricultural Science Bulletin, 2020, 36(36): 132-136.

Figures/Tables 5

References 30

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名称		序列(5’-3’)	片段大小/bp
外侧引物	AP4-F1	ATGAGTAACAATATAAAACATGAAAC	1269
外侧引物	AP4-R1	ACGATTTCGACGTTCCCCAA	1269
内侧引物	AP4-F2	TTGAGAATACGGGACGTGGG	230
内侧引物	AP4-R2	GTTAGTCATGTGAGCACCTTC	230

名称		序列(5’-3’)	片段大小/bp
外侧引物	AP4-F1	ATGAGTAACAATATAAAACATGAAAC	1269
外侧引物	AP4-R1	ACGATTTCGACGTTCCCCAA	1269
内侧引物	AP4-F2	TTGAGAATACGGGACGTGGG	230
内侧引物	AP4-R2	GTTAGTCATGTGAGCACCTTC	230