Dof Transcription Factor in Pineapple Fruits Treated with Hexaldehyde Delaying Internal Browning Resistance: Expression Analysis

doi:10.11924/j.issn.1000-6850.casb20191100794

Abstract

Abstract:

DNA binding with one finger (Dof) proteins, which are a family of plant-specific transcription factor (TF), plays important roles in diverse biotic and abiotic stress responses. The purpose of this study is to investigate the roles of Dof TF family members in internal browning (IB) resistance in the postharvest pineapple fruits. Pineapple fruits were used as materials, the pineapple fruits with IB were treated with the exogenous hexaldehyde, and then stored the treated at 25 ℃ for 8 days. Quantitative real-time PCR (qRT-PCR) technique was adopted to analyze the changes in Dof TFs gene expressions during the storage duration of fruits inducted by IB resistance. 25 putative Dof TFs which were designated as AcDof1-AcDof25, were identified and isolated based on pineapple genome database. 0.1% (v/v) hexaldehyde treatment significantly delayed the ascent of IB index of fruits compared with the controls. qRT-PCR analysis showed that 18 out of 20 AcDof TFs were up-regulated after hexaldehyde treatment. Among them, 5 TFs, such as AcDof4, AcDof8, AcDof14, AcDof16 and AcDof18 had significant expression profiles between controls and the treated fruits. However, 1 AcDof, namely AcDof13, was apparently down-regulated by hexaldehyde treatment. These 6 AcDofs might be involved in the regulation of hexaldehyde delaying IB in different ways, thus they could be the related genes of IB.

Key words: Dof, gene expression, hexaldehyde, internal browning, pineapple fruit

CLC Number:

Q786

Chen Li, Gu Hui, Jia Zhiwei, Hong Keqian. Dof Transcription Factor in Pineapple Fruits Treated with Hexaldehyde Delaying Internal Browning Resistance: Expression Analysis[J]. Chinese Agricultural Science Bulletin, 2020, 36(15): 115-122.

Figures/Tables 5

References 41

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基因	正向引物(5′-3′)	反向引物(5′-3′)
Acactin	CTGGCCTACGTGGCACTTGACTT	CACTTCTGGGCAGCGGAACCTTT
AcDof1	ACCATTGTACCCTGCACCTTAC	TTGATCCCATTTCCATCCCT
AcDof2	CAGAAGCAGAAGCAGAAGCAG	CAATGGGAAGCGATAATGGC
AcDof3	CTTGGCTTTCCCCTACCACA	GGGCACAAACGACCCTACTC
AcDof4	GCAAGAGAGGAAGGTGGTGAG	TGGAAGAGGAGTTGGATGAGG
AcDof8	AAGAATGAGAAGGTGATGGA	GGTCGGATTTAGGGCAA
AcDof10	CAACCTCTCCTTCCCACA	TCATTTCCATCACCTCCTC
AcDof11	TACGGCGAAAATGGAAGTGT	CCCCCAGAAGAAGGAAAGAAAT
AcDof12	CCGACAAAGAGCGAAGGGA	GCAGACCAAAGCAGGAGACC
AcDof13	CCGGGAGACGAAGTTCT	CGTTGTTGATGATGATGATG
AcDof14	ATCTCTGAGGAATGTCCCTGTTG	GGTGATGCTGAGGTTGGTGG
AcDof15	ACGCAACAAGCGGAGCA	TGTGGAGGAAGAGGAGGACG
AcDof16	CATCCACCATCCCTTTCCC	AACTCTTTGCCGCCTCATCT
AcDof17	ATGCCGCTCAAACTGC	CCTTCCCTCTCTCTCCTCT
AcDof18	AACCAGGGAGTAGCG	GTAGTTGTTGTAGTAGCAGA
AcDof19	ATAAGGAGGAATCAATGGAG	TTGGCGGGTAATAAGGTA
AcDof20	CTTGGGAAGGAGGCGTTTT	GAGGGTTTTTGCGGAGTTTT
AcDof21	TTGACCCCCTCATTCCACC	CTCTGTTTTTCGTTTTCCGCTT
AcDof22	TGGCTTCCCCAACCTCTACT	CCTCCTTCCAACATTGCTTTAT
AcDof23	TGCCGACACCACTGAAGACA	AAGACGACACGGAGGAGAAAG
AcDof25	CAAATCACAAGCAGCCTC	AACTATCAATCAACCACGC

基因	正向引物(5′-3′)	反向引物(5′-3′)
Acactin	CTGGCCTACGTGGCACTTGACTT	CACTTCTGGGCAGCGGAACCTTT
AcDof1	ACCATTGTACCCTGCACCTTAC	TTGATCCCATTTCCATCCCT
AcDof2	CAGAAGCAGAAGCAGAAGCAG	CAATGGGAAGCGATAATGGC
AcDof3	CTTGGCTTTCCCCTACCACA	GGGCACAAACGACCCTACTC
AcDof4	GCAAGAGAGGAAGGTGGTGAG	TGGAAGAGGAGTTGGATGAGG
AcDof8	AAGAATGAGAAGGTGATGGA	GGTCGGATTTAGGGCAA
AcDof10	CAACCTCTCCTTCCCACA	TCATTTCCATCACCTCCTC
AcDof11	TACGGCGAAAATGGAAGTGT	CCCCCAGAAGAAGGAAAGAAAT
AcDof12	CCGACAAAGAGCGAAGGGA	GCAGACCAAAGCAGGAGACC
AcDof13	CCGGGAGACGAAGTTCT	CGTTGTTGATGATGATGATG
AcDof14	ATCTCTGAGGAATGTCCCTGTTG	GGTGATGCTGAGGTTGGTGG
AcDof15	ACGCAACAAGCGGAGCA	TGTGGAGGAAGAGGAGGACG
AcDof16	CATCCACCATCCCTTTCCC	AACTCTTTGCCGCCTCATCT
AcDof17	ATGCCGCTCAAACTGC	CCTTCCCTCTCTCTCCTCT
AcDof18	AACCAGGGAGTAGCG	GTAGTTGTTGTAGTAGCAGA
AcDof19	ATAAGGAGGAATCAATGGAG	TTGGCGGGTAATAAGGTA
AcDof20	CTTGGGAAGGAGGCGTTTT	GAGGGTTTTTGCGGAGTTTT
AcDof21	TTGACCCCCTCATTCCACC	CTCTGTTTTTCGTTTTCCGCTT
AcDof22	TGGCTTCCCCAACCTCTACT	CCTCCTTCCAACATTGCTTTAT
AcDof23	TGCCGACACCACTGAAGACA	AAGACGACACGGAGGAGAAAG
AcDof25	CAAATCACAAGCAGCCTC	AACTATCAATCAACCACGC

基因	氨基酸(AA)	起始	终止	E值
AcDof1	428	114	172	2.5e-33
AcDof2	259	31	89	7.4e-33
AcDof3	408	52	110	2.8e-33
AcDof4	364	31	89	2.5e-33
AcDof5	272	72	130	9.4e-36
AcDof6	361	63	121	1.5e-35
AcDof7	180	22	83	7.3e-30
AcDof8	354	92	150	3.9e-36
AcDof9	285	45	102	9.2e-34
AcDof10	336	53	111	4.4e-36
AcDof11	259	34	92	3.6e-36
AcDof12	361	148	206	3.7e-30
AcDof13	213	85	138	4.6e-18
AcDof14	307	50	108	2.6e-36
AcDof15	363	83	141	1.3e-35
AcDof16	497	138	196	3.8e-35
AcDof17	221	117	174	3e-17
AcDof18	136	79	135	4.2e-32
AcDof19	478	131	189	7.1e-34
AcDof20	146	77	109	8e-12
AcDof21	381	97	155	1.4e-34
AcDof22	362	53	111	1.4e-33
AcDof23	212	27	85	9.3e-33
AcDof24	326	107	152	4.2e-19
AcDof25	368	35	93	7.6e-36

基因	氨基酸(AA)	起始	终止	E值
AcDof1	428	114	172	2.5e-33
AcDof2	259	31	89	7.4e-33
AcDof3	408	52	110	2.8e-33
AcDof4	364	31	89	2.5e-33
AcDof5	272	72	130	9.4e-36
AcDof6	361	63	121	1.5e-35
AcDof7	180	22	83	7.3e-30
AcDof8	354	92	150	3.9e-36
AcDof9	285	45	102	9.2e-34
AcDof10	336	53	111	4.4e-36
AcDof11	259	34	92	3.6e-36
AcDof12	361	148	206	3.7e-30
AcDof13	213	85	138	4.6e-18
AcDof14	307	50	108	2.6e-36
AcDof15	363	83	141	1.3e-35
AcDof16	497	138	196	3.8e-35
AcDof17	221	117	174	3e-17
AcDof18	136	79	135	4.2e-32
AcDof19	478	131	189	7.1e-34
AcDof20	146	77	109	8e-12
AcDof21	381	97	155	1.4e-34
AcDof22	362	53	111	1.4e-33
AcDof23	212	27	85	9.3e-33
AcDof24	326	107	152	4.2e-19
AcDof25	368	35	93	7.6e-36

基因		处理		贮藏时间
基因		处理		0 d		2 d	4 d	6 d	8 d
Dof1		对照		1.00 ± 0.024		0.54 ± 0.04^*	0.38 ± 0.045^*	0.17 ± 0.014^*	0.15 ± 0.015^*
Dof1		己醛		1.00 ± 0.084		0.74 ± 0.048	0.45 ± 0.055	0.29± 0.024	0.33 ± 0.015
Dof 2		对照		1.00 ± 0.054		0.91 ± 0.04	0.10 ± 0.045^*	0.17 ± 0.014^*	0.31 ± 0.015
Dof 2		己醛		1.00 ± 0.044		0.66 ± 0.048^*	0.89 ± 0.055	0.36 ± 0.024	0.32 ± 0.015
Dof 3		对照		1.00 ± 0.06		0.24 ± 0.04^*	0.48 ± 0.05^*	0.37 ± 0.033^*	0.23 ± 0.018
Dof 3		己醛		1.00 ± 0.08		0.58 ± 0.06	0.74 ± 0.035	1.03 ± 0.078	0.038 ± 0.0095^*
Dof 4		对照		1.00 ± 0.049		0.11 ± 0.084^*	0.07 ± 0.021^*	0.07 ± 0.0038^*	0.23 ± 0.048
Dof 4		己醛		1.00 ± 0.07		0.45 ± 0.028	1.89 ± 0. 49	4.67 ± 0.21	0.027 ± 0.012^*
Dof 8		对照		1.00 ± 0.027		0.57 ± 0.042^*	0.66 ± 0.01^*	0.85 ± 0.09^*	0.57 ± 0.07
Dof 8		己醛		1.00 ± 0.031		1.10 ± 0. 37	0.98 ± 0.09	1.71 ± 0.31^*	0.13 ± 0.01^*
Dof 10		对照		1.00 ± 0.14		0.94 ± 0.039	0.90 ± 0.089	0.57 ± 0.08^*	1.31 ± 0.15^*
Dof 10		己醛		1.00 ± 0.13		0.92 ± 0.046	0.94 ± 0.05	2.18 ± 0.25	1.74 ± 0.26
Dof 11		对照		1.00 ± 0.078		1.11 ± 0.12^*	1.61 ± 0.34	0.92 ± 0.28^*	0.76 ± 0.15^*
Dof 11		己醛		1.00 ± 0.089		1.68 ± 0.18	1.74 ± 0.15	1.36 ± 0.17	1.84 ± 0.14
Dof 12		对照		1.00 ± 0.044		1.19 ± 0.086	1.51 ± 0.15	0.57 ± 0.036	0.40 ± 0.025
Dof 12		己醛		1.00 ± 0.037		1.13 ± 0.076	1.38 ± 0.13	0.63 ± 0.028	0.44 ± 0.039
Dof 13		对照		1.00 ± 0.29		0.61 ± 0.049	0.54 ± 0.014	2.16 ± 0.48	2.29 ± 0.18
Dof 13		己醛		1.00 ± 0.034		0.29 ± 0.024^*	0.28 ± 0.042^*	1.39 ± 0.21^*	0.53 ± 0.077^*
Dof 14		对照		1.00 ± 0.035		1.00 ± 0.061^*	0.73 ± 0.14^*	0.44 ± 0.025^*	0.25 ± 0.069^*
Dof 14		己醛		1.00 ± 0.48		1.21 ± 0.14	1.03 ± 0.038	0.68 ± 0.087	0.90 ± 0.041
Dof 15		对照		1.00 ± 0.045		1.00 ± 0.21^*	1.05 ± 0.018	0.93 ± 0.014^*	0.44 ± 0.015^*
Dof 15		己醛		1.00 ± 0.051		1.33 ± 0.11	1.09 ± 0.087	1.61 ± 0.13	0.61 ± 0.015
Dof 16		对照		1.00 ± 0.038		1.59 ± 0.013^*	0.42 ± 0.012^*	0.26 ± 0.014^*	0.60 ± 0.015^*
Dof 16		己醛		1.00 ± 0.044		2.42 ± 0.011	1.33 ± 0.021	0.85 ± 0.011	0.80 ± 0.013
Dof 17		对照		1.00 ± 0.071		0.94 ± 0.045^*	0.61 ± 0.021^*	0.53 ± 0.014^*	0.58 ± 0.021^*
Dof 17		己醛		1.00 ± 0.074		1.02 ± 0.018	0.82 ± 0.014	0.88 ± 0.017	0.67 ± 0.011
Dof 18		对照		1.00 ± 0.15		0.95 ± 0.014^*	1.13 ± 0.017^*	1.59 ± 0.014^*	1.30 ± 0.022
Dof 18		己醛		1.00 ± 0.23		4.12 ± 0.047	2.08 ± 0.028	4.91 ± 0.016	0.11 ± 0.011^*
Dof 19	对照		1.00 ± 0.075		1.77 ± 0.044		2.24 ± 0.024^*	1.33 ± 0.054^*	1.27 ± 0.018
Dof 19	己醛		1.00 ± 0.025		0.54 ± 0.0014^*		2.90 ± 0.047	2.07 ± 0.061	1.14 ± 0.024^*
Dof 20	对照		1.00 ± 0.035		0.81 ± 0.014		0.90 ± 0.021^*	0.47 ± 0.011^*	0.62 ± 0.024
Dof 20	己醛		1.00 ± 0.026		0.86 ± 0.013		2.43 ± 0.34	1.69 ± 0.14	0.51 ± 0.021^*
Dof 21	对照		1.00 ± 0.14		1.54 ± 0.017		1.11 ± 0.008^*	0.36 ± 0.014^*	0.18 ± 0.032^*
Dof 21	己醛		1.00 ± 0.015		1.48 ± 0.047		1.41 ± 0.009	0.73 ± 0.031	0.86 ± 0.025
Dof 22	对照		1.00 ± 0.023		0.49 ± 0.021		0.45 ± 0.014^*	0.23 ± 0.022^*	0.31 ± 0.025^*
Dof 22	己醛		1.00 ± 0.024		0.56 ± 0.033		0.64 ± 0.049	0.59 ± 0.078	0.45 ± 0.034
Dof 23	对照		1.00 ± 0.035		0.51 ± 0.014		0.64 ± 0.021^*	0.31 ± 0.017^*	0.20 ± 0.014
Dof 23	己醛		1.00 ± 0.25		0.54 ± 0.074		0.76 ± 0.014	0.70 ± 0.045	0.053 ± 0.0017^*
Dof 25	对照		1.00 ± 0.085		3.06 ± 0.24		1.38 ± 0.014^*	0.19 ± 0.0016^*	0.57 ± 0.016
Dof 25	己醛		1.00 ± 0.075		2.04 ± 0.17^*		1.79 ± 0.14	1.23 ± 0.017	0.31 ± 0.0024^*