BvM14-Dof3.4 Gene in Sugar Beet M14 Line: Cloning and Expression in Response to Salt Stress

doi:10.11924/j.issn.1000-6850.casb20191200897

Abstract

Abstract:

The objective is to study the function of BvM14-Dof3.4 gene in response to salt stress. The full-length sequence of BvM14-Dof3.4 gene from the root of sugar beet M14 line was obtained by PCR and analyzed by bioinformatics. The response mode of BvM14-Dof3.4 gene under salt stress was also verified. The results showed that the largest ORF of the gene was 408 bp which encoded 135 amino acids, the molecular weight of the protein was 12646.63 Da, the theoretical pI was 4.68, the protein was hydrophilic protein; the secondary and tertiary structure of the protein were mainly composed of extension chain and random curl; the amino acid sequence similarity between BvM14-Dof3.4 and Dof3.4 from Beta vulgaris database was up to 99.26%. The phylogenetic tree showed that BvM14-Dof3.4 protein was highly homologous with Dof3.4 protein from Spinacia oleracea. According to the transcriptome database from the root of sugar beet M14 line under salt stress, BvM14-Dof3.4 gene was involved in the response to salt stress, which was up-regulated by 2.05 times under 200 mmol/L NaCl and 1.41 times under 400 mmol/L NaCl. The full cDNA length of BvM14-Dof3.4 gene was successfully obtained in this experiment and its response to salt stress was determined. The results have certain significance for the exploration of high quality genes in sugar beet M14 line and the improvement of stress resistance of cultivated sugar beet, and lay a foundation for further research on the function of BvM14-Dof 3.4 gene in response to salt stress.

Key words: sugar beet M14 line, BvM14-Dof3.4 gene, clone, salt stress, response

CLC Number:

S566.3

Ma Chunquan, Huang Yuanyuan, Li Haiying. BvM14-Dof3.4 Gene in Sugar Beet M14 Line: Cloning and Expression in Response to Salt Stress[J]. Chinese Agricultural Science Bulletin, 2020, 36(13): 36-41.

Figures/Tables 9

References 33

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引物名称	序列
BvM14-Dof3.4-S	5'-ATGGGTGGAGGCGGAGGC-3'
BvM14-Dof3.4-AS	5'-TCACTTGAGACCATTGGCTGATG-3'

引物名称	序列
BvM14-Dof3.4-S	5'-ATGGGTGGAGGCGGAGGC-3'
BvM14-Dof3.4-AS	5'-TCACTTGAGACCATTGGCTGATG-3'