Studies on Secondary Metabolites of Cannabis sativa L. Endophytic Fungus HMY07 Based on Flavonoid Biosynthesis

doi:10.11924/j.issn.1000-6850.casb2024-0271

Abstract

Abstract:

Based on the flavonoids biosynthesis, the flavonoids in the fermentation of Cannabis sativa L. endophytic fungus HMY07 (Alternaria alternata) were extracted and separated. The antimicrobial activities of the fermentation of Cannabis sativa L. endophytic fungus HMY07 was analyzed using Oxford cup method. Additionally, the antioxidant activities of the fermentation were measured by DPPH method. After analyzing the secondary metabolites of endophytic fungus HMY07 using HPLC, D₁₀₁ macroporous adsorption resin column, silica gel chromatography, polyamide column, and recrystallization were applied to separate the secondary metabolites in the endophytic fungal fermentation. The fermentation of Cannabis sativa L. endophytic fungus HMY07 showed good antimicrobial and antioxidant activities, and seven secondary metabolites were isolated from the fermentation. These metabolites are quercetin (A), kaempferol (B), luteolin (C), cinnamic acid (D), ferulic acid (E), apigenin (F), and hyperoside (G). The Cannabis sativa L. endophytic fungus HMY07 can produce a variety of flavonoid secondary metabolites, making it a valuable endophytic fungus for further development and application, with all isolated compounds reported for the first time from this strain.

Key words: Cannabis sativa L., endophytic fungus, Alternaria alternata HMY07, antimicrobial activities, antioxidant activities, flavonoids

CONG Tianyue, XING Xiangyu, YOU Mengyao, CHENG Li, ZHENG Chunying. Studies on Secondary Metabolites of Cannabis sativa L. Endophytic Fungus HMY07 Based on Flavonoid Biosynthesis[J]. Chinese Agricultural Science Bulletin, 2025, 41(5): 56-62.

Figures/Tables 3

References 33

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样品	A	B	C	D	E	F
HMY07发酵液	12.25±0.20	7.25±0.22	12.96±0.13	9.50±0.22	11.72±0.23	11.25±0.23
甲醇	-	-	-	-	-	-
Str (100 μg/mL)	22.00±0.30	20.50±0.32	18.50±0.25	21.00±0.21	22.55±0.15	24.00±0.55
Nys (100 μg/mL)	-	-	-	-	-	-
样品	G	H	I	J	K
HMY07发酵液	14.88±0.15	8.50±0.22	10.07±0.18	9.02±0.35	12.25±0.30
甲醇	-	-	-	-	-
Str (100 μg/mL)	19.25±0.51	22.00±0.20	20.50±0.22	17.25±0.41	-
Nys (100 μg/mL)	-	-	-	-	22.00±0.30

样品	A	B	C	D	E	F
HMY07发酵液	12.25±0.20	7.25±0.22	12.96±0.13	9.50±0.22	11.72±0.23	11.25±0.23
甲醇	-	-	-	-	-	-
Str (100 μg/mL)	22.00±0.30	20.50±0.32	18.50±0.25	21.00±0.21	22.55±0.15	24.00±0.55
Nys (100 μg/mL)	-	-	-	-	-	-
样品	G	H	I	J	K
HMY07发酵液	14.88±0.15	8.50±0.22	10.07±0.18	9.02±0.35	12.25±0.30
甲醇	-	-	-	-	-
Str (100 μg/mL)	19.25±0.51	22.00±0.20	20.50±0.22	17.25±0.41	-
Nys (100 μg/mL)	-	-	-	-	22.00±0.30