An Aroma-producing Endophytic Fungus RP2 from Glycyrrhiza uralensis: Preliminary Identification and Volatile Components' Analysis

doi:10.11924/j.issn.1000-6850.casb2021-0668

Abstract

Abstract:

The study aims to explore secondary metabolite resources of endophytic fungus RP2 which is isolated from Glycyrrhiza uralensis and increase the accessing ways for volatile aroma components. Endophytic fungus RP2 was chosen as the object and identified by morphological observation and ITS sequence analysis, and its volatile components were analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that endophytic fungus RP2 was identified as Fusarium oxysporum. 11 volatile components were isolated and identified from the RP2 fermentation liquid, and 6 components were isolated and identified from RP2 mycelium. RP2 can produce a variety of volatile aroma components that can be widely used in current industrial production, such as 2-phenylethanol. This study is of great significance for the development and utilization of RP2.

Key words: endophytic fungus RP2 from Glycyrrhiza uralensis, morphological observation, ITS sequence analysis, GC-MS, volatile components

CLC Number:

S682.31

YOU Mengyao, YAN Jiajia, WAN Lu, ZHANG He, ZHENG Chunying. An Aroma-producing Endophytic Fungus RP2 from Glycyrrhiza uralensis: Preliminary Identification and Volatile Components' Analysis[J]. Chinese Agricultural Science Bulletin, 2022, 38(12): 138-145.

Figures/Tables 7

References 16

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序号	保留时间/min	分子式	化合物名称	相对含量/%
1	10.34	C₆H₈O₄	3,5-二羟基-6-甲基-2,3-二氢吡喃-4-酮	1.80
2	13.22	C₆H₆O₃	5-(羟甲基)呋喃-2-甲醛	1.00
3	18.51	C₁₅H₃₂	2,6,11-三甲基十二烷	0.42
4	31.98	C₁₀H₁₆O₂	(R)-6-戊基-5,6-二氢吡喃-2-酮	0.36
5	83.57	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	49.12
6	205.04	C₄₂H₆₃O₄P	磷酸三（2,4-二叔丁基苯基）酯	0.97

序号	保留时间/min	分子式	化合物名称	相对含量/%
1	10.34	C₆H₈O₄	3,5-二羟基-6-甲基-2,3-二氢吡喃-4-酮	1.80
2	13.22	C₆H₆O₃	5-(羟甲基)呋喃-2-甲醛	1.00
3	18.51	C₁₅H₃₂	2,6,11-三甲基十二烷	0.42
4	31.98	C₁₀H₁₆O₂	(R)-6-戊基-5,6-二氢吡喃-2-酮	0.36
5	83.57	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	49.12
6	205.04	C₄₂H₆₃O₄P	磷酸三（2,4-二叔丁基苯基）酯	0.97

序号	保留时间/min	分子式	化合物名称	相对含量/%
1	9.53	C₈H₁₀O	2-苯基乙醇	3.00
2	17.49	C₈H₁₀O₂	1-苯基乙烷-1,2-二醇	1.60
3	24.51	C₆H₆N₂O	尼克酰胺	0.54
4	27.15	C₈H₁₀O₂	对羟基苯乙酸	1.04
5	32.70	C₁₄H₄₂O₇Si₇	2,2,4,4,6,6,8,8,10,10,12,12,14,14-十四甲基-1,3,5,7,9,11,13-七氧杂-2,4,6,8,10,12,14-七硅杂环十四烷	0.23
6	36.54	C₁₄H₂₂O	2,4-二-叔-丁基苯酚	0.33
7	48.49	C₈H₈O₃	2-(4-羟基苯基)乙酸	0.96
8	77.20	C₁₀H₉NO₂	2-(1H-吲哚-1-基)乙酸	0.84
9	83.37	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	28.00
10	116.12	C₂₁H₃₀O₂	2-((1R,6R)-3-甲基-6-(丙-1-烯-2-基)环己-2-烯基)-5-戊基苯-1,3-二酚	0.69
11	157.61	C₂₂H₄₃NO	芥酰胺	1.31

序号	保留时间/min	分子式	化合物名称	相对含量/%
1	9.53	C₈H₁₀O	2-苯基乙醇	3.00
2	17.49	C₈H₁₀O₂	1-苯基乙烷-1,2-二醇	1.60
3	24.51	C₆H₆N₂O	尼克酰胺	0.54
4	27.15	C₈H₁₀O₂	对羟基苯乙酸	1.04
5	32.70	C₁₄H₄₂O₇Si₇	2,2,4,4,6,6,8,8,10,10,12,12,14,14-十四甲基-1,3,5,7,9,11,13-七氧杂-2,4,6,8,10,12,14-七硅杂环十四烷	0.23
6	36.54	C₁₄H₂₂O	2,4-二-叔-丁基苯酚	0.33
7	48.49	C₈H₈O₃	2-(4-羟基苯基)乙酸	0.96
8	77.20	C₁₀H₉NO₂	2-(1H-吲哚-1-基)乙酸	0.84
9	83.37	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	28.00
10	116.12	C₂₁H₃₀O₂	2-((1R,6R)-3-甲基-6-(丙-1-烯-2-基)环己-2-烯基)-5-戊基苯-1,3-二酚	0.69
11	157.61	C₂₂H₄₃NO	芥酰胺	1.31

序号	保留时间/min	分子式	化合物名称	相对含量/%
1	13.99	C₈H₈O₂	2-羟基-1-苯基乙酮	0.67
2	19.53	C₈H₁₀O₂	苯乙二醇	34.42
3	62.89	C₁₇H₂₄O₄	邻苯二甲酸5-甲基-2-乙基酯	9.54
4	82.87	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	5.50
5	116.18	C₂₁H₃₀O₂	2-((1R,6R)-3-甲基-6-(丙-1-烯-2-基)环己-2-烯基)-5-戊基苯-1,3-二酚	0.29
6	157.80	C₂₂H₄₃NO	芥酰胺	1.36