Photosynthetic Variation and Cold Resistance in New Sugarcane Varieties Exposed to Chilling Stress

doi:10.11924/j.issn.1000-6850.casb2024-0308

Abstract

Abstract:

To evaluate the cold resistance of newly released ‘Zhongzhe’ (ZZ) cultivars (‘ZZ 9’, ‘ZZ 14’, ‘ZG 1’ and ‘ZZFN 14-1854’), measurements were taken on sugarcane, and the physiological indices of leaves were analyzed following exposure to low temperatures. The results indicated that significant differences existed in the photosynthetic physiological indices of the four sugarcane varieties after low-temperature chilling. ‘ZZ 14’ exhibited relatively higher values in multiple parameters, namely Fv/Fm, Fv/Fo, net photosynthetic rate, photosynthetic pigment content, SPAD values (chlorophyll content), anthocyanin content, and superoxide dismutase (SOD) activity. In contrast, ‘ZG 1’ manifested lower values across these indicators, implying weaker cold tolerance. The other two cultivars demonstrated intermediate performance. It is indicated that ‘ZZ 14’ improves its cold resistance by increasing anthocyanin levels and SOD activity, thereby exhibiting superior cold tolerance. Conversely, ‘ZG 1’ presents relatively inferior cold resistance. The ranking of the cold resistance of the varieties from highest to lowest was ‘ZZ 14’> ‘ZZ 9’> ‘ZZFN 14-1854’> ‘ZG 1’.

Key words: sugarcane, cold resistance, photosynthesis, fluorescence parameter, chlorophyll, anthocyanin, SOD activity, principal component analysis

LI Huojian, XU Yuzhi, CHEN Jun, ZHANG Chi, WU Yuling, WEI Yibin, JIANG Chunxiu, ZENG Lifang, ZHANG Muqing. Photosynthetic Variation and Cold Resistance in New Sugarcane Varieties Exposed to Chilling Stress[J]. Chinese Agricultural Science Bulletin, 2025, 41(16): 1-8.

Figures/Tables 10

References 48

[1]	许文花, 杨清辉. 甘蔗抗寒性研究进展[J]. 甘蔗, 2003(3):8-12.
[2]	邓展云, 刘海斌, 张革民, 等. 2007—2008年榨季广西甘蔗霜冻发生危害规律的调查[J]. 中国糖料, 2009(1):47-50.
[3]	唐仕云, 杨丽涛, 李杨瑞. 低温胁迫下不同甘蔗品种(系)光合特性的变化及其与耐寒性的关系[J]. 广西植物, 2012, 32(5):679-685.
[4]	谭宗琨, 黄城华, 孟翠丽, 等. 甘蔗寒冻害等级指标及灾损指标的初步研究[J]. 中国农学通报, 2014, 30(28):169-181. doi: 10.11924/j.issn.1000-6850.2014-0744
[5]	PERERA-CASTRO A V, BRITO P, GONZÁLEZ-RODRÍGUEZ A M. Changes in thermic limits and acclimation assessment for an alpine plant by chlorophyll fluorescence analysis: Fv/Fm vs. Rfd[J]. Photosynthetica, 2018, 56(2):527-536.
[6]	SNIDER J L, THANGTHONG N, PILON C, et al. OJIP-fluorescence parameters as rapid indicators of cotton (Gossypium hirsutum L.) seedling vigor under contrasting growth temperature regimes[J]. Plant physiology and biochemistry, 2018,132:249-257.
[7]	李心, 杨柳燕, 张栋梁, 等. 低温胁迫下2个红掌新种质的耐寒性鉴定[J]. 分子植物育种, 2019, 17(16):5446-5453.
[8]	SHIN Y K, BHANDARI S R, LEE J G. Monitoring of salinity, temperature, and drought stress in grafted watermelon seedlings using chlorophyll fluorescence[J]. Frontiers in plant science, 2021,12:786309.
[9]	田鑫, 钟程, 李性苑. 春大豆苗期低温胁迫下叶绿素荧光响应[J]. 安徽农业科学, 2019, 47(24):28-31.
[10]	PEREZ-LLORCA M, CASADESUS A, MUNNE-BOSCH S, et al. Contrasting patterns of hormonal and photoprotective isoprenoids in response to stress in Cistus albidus during a Mediterranean winter[J]. Planta, 2019, 250(5):1409-1422.
[11]	孙富, 杨丽涛, 谢晓娜, 等. 低温胁迫对不同抗寒性甘蔗品种幼苗叶绿体生理代谢的影响[J]. 作物学报, 2012, 38(4):732-739.
[12]	XU F, HE L, GAO S, et al. Comparative analysis of two sugarcane ancestors Saccharum officinarum and S. spontaneum based on complete chloroplast genome sequences and photosynthetic ability in cold stress[J]. International journal of molecular sciences, 2019, 20(15):3828.
[13]	吴广霞, 唐献龙, 杨德光, 等. 植物低温胁迫生理研究进展[J]. 作物杂志, 2008(3):17-19.
[14]	张超, 梁峥, 贾民隆, 等. 秋季露地低温对野生和栽培萱草光合特性的影响[J]. 北方园艺, 2020(18):78-86.
[15]	赵海民. 低温处理对紫丁香幼苗生理特性的影响[J]. 农业科技通讯, 2020(11):161-162.
[16]	XU H Y, HUANG C L, JIANG X, et al. Impact of cold stress on leaf structure, photosynthesis, and metabolites in Camellia weiningensis and C. oleifera seedlings[J]. Horticulturae, 2022, 8(6):494-494.
[17]	朱俊杰, 王天顺, 牙禹, 等. 低温下外源腐胺对黑皮果蔗拔地拉光合性能的影响[J]. 西南农业学报, 2018, 31(10):2069-2074.
[18]	王海玲, 张雅芳, 段维兴, 等. 甘蔗光合低温耐受性及其与抗旱性的关联[J]. 生态学杂志, 2021, 40(11):3577-3584.
[19]	OWUSU A M, MA J, LUO R, et al. Transcriptome analyses revealed chilling response genes in mango (Mangifera indica L. cv. Keitt) leaf[J]. Journal of plant interactions, 2023, 18(1):217-226.
[20]	GU K, HOU S, CHEN J, et al. The physiological response of different tobacco varieties to chilling stress during the vigorous growing period[J]. Scientific reports, 2021, 11(1):22136. doi: 10.1038/s41598-021-01703-7 pmid: 34764409
[21]	GHABEL V K, KARAMIAN R. Effects of TiO₂ nanoparticles and spermine on antioxidant responses of Glycyrrhiza glabra L. to cold stress[J]. Acta botanica croatica, 2020, 79(2):137-147.
[22]	VERMA K K, SONG X P, ZENG Y, et al. Foliar application of silicon boosts growth, photosynthetic leaf gas exchange, antioxidative response and resistance to limited water irrigation in sugarcane (Saccharum officinarum L.)[J]. Plant physiology and biochemistry, 2021,166:582-592.
[23]	DENG Z N, HUANG H R, HUANG C M, et al. Short-term limited water irrigation influences the photosynthetic pigments, enzymatic and non-enzymatic activities in Saccharum spontaneum L. at vegetative stage[J]. Sugar tech, 2023, 25(1):110-118.
[24]	朱鹏锦, 庞新华, 谭秦亮, 等. 低温胁迫对不同甘蔗品种叶片光合色素和光合生理特性的影响[J]. 热带作物学报, 2019, 40(5):875-881. doi: 10.3969/j.issn.1000-2561.2019.05.007
[25]	唐仕云, 杨丽涛, 李杨瑞. 不同甘蔗基因型对低温胁迫的响应及其耐寒性评价[J]. 热带作物学报,2012,33(4):635-641.
[26]	JAIN R. Changes in photosynthetic pigment contents and SPAD index in relation to waterlogging in sugarcane[J]. Plant cell biotechnology and molecular biology. 2022, 23(19/20):43-48.
[27]	XU P, ROGERS S J, ROOSSINCK M J. Expression of antiapoptotic genes bcl-xL and ced-9 in tomato enhances tolerance to viral-induced necrosis and abiotic stress[J]. Proceedings of the national academy of sciences, 2004, 101(44):15805-15810.
[28]	周露, 刘杨, 崔群香, 等. 茄子低温响应基因SmICE1的克隆及其功能分析[J]. 南京农业大学学报, 2022, 45(2):244-253.
[29]	ZHANG Y, QU X, LI X, et al. Comprehensive transcriptome and WGCNA analysis reveals the potential function of anthocyanins in low-temperature resistance of a red flower mutant tobacco[J]. Genomics, 2023, 115(6):110728.
[30]	张彬, 李萌, 蒋茂双, 等. 花青素积累与谷子低温胁迫响应的关系[J]. 核农学报, 2020, 34(11):2607-2613. doi: 10.11869/j.issn.100-8551.2020.11.2607
[31]	董亮, 何永志, 王远亮, 等. 超氧化物歧化酶(SOD)的应用研究进展[J]. 中国农业科技导报, 2013, 15(5):53-58.
[32]	丁释丰, 袁森, 黄稚清, 等. 低温胁迫对红果风铃木幼苗生理特性的影响[J]. 广西植物, 2020, 40(8):1151-1158.
[33]	SUN B, LIU G L, PHAN T T, et al. Effects of cold stress on root growth and physiological metabolisms in seedlings of different sugarcane varieties[J]. Sugar tech, 2017, 19(2):165-175.
[34]	于晓南. 植物叶片中花青素的分析与研究[J]. 现代仪器, 2000(4):37-38.
[35]	WÓJCIK-JAGLA M, RAPACZ M. Freezing tolerance and tolerance to de-acclimation of European accessions of winter and facultative barley[J]. Scientific reports, 2023, 13(1).
[36]	杨程, 杜思梦, 张德奇, 等. 基于叶绿素荧光的小麦越冬期冻害评价[J]. 作物杂志, 2022(1):154-160.
[37]	HAZRATI S, TAHMASEBI-SARVESTANI Z, MODARRES-SANAVY S A, et al. Effects of water stress and light intensity on chlorophyll fluorescence parameters and pigments of Aloe vera L.[J]. Plant physiology & biochemistry, 2016,106:141-148.
[38]	SONG Q, ZHANG S, BAI C, et al. Exogenous Ca²⁺ priming can improve peanut photosynthetic carbon fixation and pod yield under early sowing scenarios in the field[J]. Frontiers in plant science, 2022,13:1004721.
[39]	CHANG C Y, UNDA F, ZUBILEWICH A, et al. Sensitivity of cold acclimation to elevated autumn temperature in field-grown Pinus strobus seedlings[J]. Frontiers in plant science, 2015,6:165.
[40]	王海玲. 甘蔗叶片结构功能及其抗寒性的关联分析[D]. 南宁: 广西大学, 2021.
[41]	陈根云, 陈娟, 许大全. 关于净光合速率和胞间CO₂浓度关系的思考[J]. 植物生理学通讯, 2010, 46(1):64-66.
[42]	李茂枝. 浅谈甘蔗抗寒性及防寒措施[J]. 中国糖料, 1998(2):43-46.
[43]	SAKAIGAICHI T, TSUCHIDA H, ADACHI K, et al. Phenological changes in the chlorophyll content and its fluorescence in field-grown sugarcane clones under over-wintering conditions[J]. Sugar tech, 2019, 21(5):843-846.
[44]	SHAO L, SHU Z, SUN S L, et al. Antioxidlation of anthocyanins in photosynthesis under high temperature stress[J]. Journal of integrative plant biology, 2007, 49(9):1341-1351.
[45]	李旭娟, 毛钧, 赵培方, 等. 美国引进甘蔗热带种的ARMSPCR真实性鉴定[J]. 中国糖料, 2023, 45(4):24-29.
[46]	王平, 余凡, 黄永吉, 等. 染色体计数法鉴定甘蔗真假热带种[J]. 亚热带农业研究, 2015, 11(3):160-163.
[47]	陈如凯. 现代甘蔗育种的理论与实践[M]. 北京: 中国农业出版社,2003:19-35.
[48]	HERMANN S R, AITKEN K S, JACKSON P A, et al. Evidence for second division restitution as the basis for 2n+n maternal chromosome transmission in a sugarcane cross[J]. Euphytica, 2012, 187(3):359-368.

品种	Fo	Fm	Fv/Fm	Fv/Fo
中蔗9号	0.29±0.034a	0.97±0.12a	0.7±0.03a	2.32±0.28b
中蔗14号	0.25±0.032b	0.93±0.12a	0.73±0.01a	2.65±0.18a
中果1号	0.079±0.03d	0.14±0.10c	0.37±0.12c	0.67±0.44d
中蔗福农14-1854	0.18±0.05c	0.45±0.19b	0.57±0.1b	1.45±0.6c

品种	Fo	Fm	Fv/Fm	Fv/Fo
中蔗9号	0.29±0.034a	0.97±0.12a	0.7±0.03a	2.32±0.28b
中蔗14号	0.25±0.032b	0.93±0.12a	0.73±0.01a	2.65±0.18a
中果1号	0.079±0.03d	0.14±0.10c	0.37±0.12c	0.67±0.44d
中蔗福农14-1854	0.18±0.05c	0.45±0.19b	0.57±0.1b	1.45±0.6c

品种	净光合速率(Pn)/[μmol/(m²·s)]	蒸腾速率(Tr)/[mmol/(m²·s)]	胞间二氧化碳浓度(Ci)/(mg/kg)	瞬时水分利用效率(WUE_inst)/(μmol/mol)
中蔗9号	10.48±0.73a	0.90±0.086a	288.10±4.07ab	11.77±1.72b
中蔗14号	12.24±0.81a	0.43±0.004c	281.83±22.11b	28.56±1.69a
中果1号	1.89±1.14b	0.70±0.08b	299.81±4.88ab	2.81±1.78c
中蔗福农14-1854	1.66±1.25b	0.41±0.10c	311.07±11.04a	4.47±3.74c

品种	净光合速率(Pn)/[μmol/(m²·s)]	蒸腾速率(Tr)/[mmol/(m²·s)]	胞间二氧化碳浓度(Ci)/(mg/kg)	瞬时水分利用效率(WUE_inst)/(μmol/mol)
中蔗9号	10.48±0.73a	0.90±0.086a	288.10±4.07ab	11.77±1.72b
中蔗14号	12.24±0.81a	0.43±0.004c	281.83±22.11b	28.56±1.69a
中果1号	1.89±1.14b	0.70±0.08b	299.81±4.88ab	2.81±1.78c
中蔗福农14-1854	1.66±1.25b	0.41±0.10c	311.07±11.04a	4.47±3.74c

品种	叶绿素a含量/(mg/L)	叶绿素b含量/(mg/L)	类胡萝卜素含量/(mg/L)	SPAD值
中蔗9号	6.71±0.74a	1.93±0.25a	0.98±0.09a	29.85±3.09b
中蔗14号	7.74±1.47a	2.27±0.47a	1.1±0.28a	39.51±3.42a
中果1号	0.73±0.13b	0.14±0.04b	0.22±0.05b	3.14±1.98d
中蔗福农14-1854	0.8±0.21b	0.26±0.16b	0.14±0.003b	7.25±2.27c