Isolation and Identification of Glarea lozoyensis P1 and Antagonistic and Detoxification Activity Against Fusarium graminearum

doi:10.11924/j.issn.1000-6850.casb2024-0431

Abstract

Abstract:

This study aims to screen biocontrol strains against Fusarium graminearum species complex in order to provide new biocontrol resources for the sustainable management of fusarium head blight. A high-efficiency biocontrol strain was isolated from non-agricultural soil samples collected in Badong County, Enshi, Hubei Province, using the dilution plate method with Fusarium graminearum as the indicator strain. The inhibitory effects of the isolate Glarea lozoyensis fermentation broth on mycelial growth and spore germination of F. graminearum were assessed,as well as its degradation rates on deoxynivalenol (DON) and zearalenone (ZEN) in vitro. The strain was identified through morphological characteristics and 18S rRNA gene sequence analysis. Further experiments were conducted to assess control efficacy in field plots. The results showed that a 10-fold dilution of its fermentation broth inhibited F. graminearum mycelial growth and spore germination by 91.98% and 100%, respectively, and achieved 100% degradation rates for DON and ZEN. Morphological characteristics and 18S rRNA gene analysis identified as Glarea lozoyensis (GL), designated GL-P1. Field trial results demonstrated that the fermentation broth of GL-P1 achieved a 71.36% control efficacy against Fusarium head blight, comparable to the fungicide tebuconazole. The above study indicates that Glarea lozoyensis P1 is a biocontrol isolate with promising field efficacy against Fusarium head blight, showing potential for further application and development.

Key words: fusarium head blight, Fusarium graminearum, antimicrobial, Glarea lozoyensis P1, mycelial inhibition, detoxifying mycotoxins

GUO Haozhen, ZHOU Huazhong, DENG Chunlin, LI Yan, YANG Lijun, GONG Shuangjun. Isolation and Identification of Glarea lozoyensis P1 and Antagonistic and Detoxification Activity Against Fusarium graminearum[J]. Chinese Agricultural Science Bulletin, 2025, 41(12): 107-112.

Figures/Tables 5

References 29

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植物病原菌	抑菌率/%
灰葡萄孢菌 Botrytis cinerea	77.08±2.75c
长蠕孢菌 Bipolaris maydis	72.34±1.65c
黑腐皮壳菌 Valsa mali	83.92±0.66b
核盘菌 Sclerotinia sclerotiorum	60.22±0.62e
茄链格孢菌 Alternaria solani	59.18±1.37e
禾谷镰刀菌 Fusarium graminearum	91.98±0.24a
辣椒刺盘孢菌 Colletotrichum capsici	67.44±0.09d
多主棒孢霉菌Corynespora cassiicola	55.81±1.26f
刚毛球腔菌 Setosphaeria turcica	82.65±0.83b
链核盘菌 Monilinia fructicola	83.91±0.56b
灰梨孢菌Pyricularia oryzae	51.11±0.74g

植物病原菌	抑菌率/%
灰葡萄孢菌 Botrytis cinerea	77.08±2.75c
长蠕孢菌 Bipolaris maydis	72.34±1.65c
黑腐皮壳菌 Valsa mali	83.92±0.66b
核盘菌 Sclerotinia sclerotiorum	60.22±0.62e
茄链格孢菌 Alternaria solani	59.18±1.37e
禾谷镰刀菌 Fusarium graminearum	91.98±0.24a
辣椒刺盘孢菌 Colletotrichum capsici	67.44±0.09d
多主棒孢霉菌Corynespora cassiicola	55.81±1.26f
刚毛球腔菌 Setosphaeria turcica	82.65±0.83b
链核盘菌 Monilinia fructicola	83.91±0.56b
灰梨孢菌Pyricularia oryzae	51.11±0.74g

处理	抑制率/%
发酵液稀释5倍	100^a
发酵液稀释10倍	100^a
发酵液稀释50倍	95.23±1.25^b
对照	0

处理	抑制率/%
发酵液稀释5倍	100^a
发酵液稀释10倍	100^a
发酵液稀释50倍	95.23±1.25^b
对照	0

处理	病情指数	防治效果/%
清水对照	53.23±1.32a
10倍稀释液	15.24±1.17d	71.36a
50倍稀释液	24.56±2.14c	53.86b
100倍稀释液	34.65±1.26b	34.91c
430克/升戊唑醇	13.28±1.03d	75.05a