Research of Resistance in ‘Zhoumai’ Wheat Cultivars to Fusarium Head Blight (FHB) with Fhb1 Gene

doi:10.11924/j.issn.1000-6850.casb20200300201

Abstract

Abstract:

To accelerate the improvement of wheat resistance to fusarium head blight (FHB) in ‘Zhoumai’ wheat, we used the local main wheat varieties (lines) ‘Zhoumai 22’, ‘Zhoumai 32’ and ‘Zhou11550’ as the female parents and the ‘Ningmai 9’, ‘Shengxuan 6’, ‘Yangmai 21’, which are the FHB resistant materials in the middle and lower reaches of the Yangtze River, as male parents to make a series of hybrid combinations. Then we got 621 selected materials in F₃-F₆ offspring. In the field, we used different FHB strains from Jiangsu and Henan to identify the resistance to FHB through inoculating spores into spikelets by single flower drip method. Meanwhile, the molecular detection of the progenitor materials was carried out by using the closely linked diagnostic marker His-InDel of the main gene Fhb1. Field inoculation results showed that 23.9% of the total offspring from Jiangsu had high or moderate resistance to FHB, while this rate was 35.1% in the offspring of Henan. The resistance of the offspring materials was significantly improved compared with their parents, which were susceptible to FHB. And the resistance of the materials to strains from Jiangsu was lower than that from Henan. Molecular detection results showed that the resistance to FHB had a significant difference in materials containing Fhb1 or not, indicating that Fhb1 gene marker assisted selection could be used to improve the resistance of wheat cultivars to FHB efficiently.

Key words: wheat, fusarium head blight (FHB), Fhb1 gene, molecular identification, field resistance

CLC Number:

Li Nannan, Li Shuncheng, Han Yulin, Zou Shaokui, Du Xiaoyu, Yang Guangyu, Wang Lina, Zhang Qian, Lv Yongjun. Research of Resistance in ‘Zhoumai’ Wheat Cultivars to Fusarium Head Blight (FHB) with Fhb1 Gene[J]. Chinese Agricultural Science Bulletin, 2021, 37(4): 98-104.

Figures/Tables 6

References 34

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抗性	Fhb1	不含Fhb1
抗赤材料数	210	411
高抗	48	1
中抗	119	50
中感	34	206
高感	9	154

抗性	Fhb1	不含Fhb1
抗赤材料数	210	411
高抗	48	1
中抗	119	50
中感	34	206
高感	9	154

变异来源	df	SS	s²	F	F_0.05	F_0.01
处理间	1	25.74	25.74	45.81^**	3.86	6.69
处理内	619	168.04	0.56
总变异	620	193.79

变异来源	df	SS	s²	F	F_0.05	F_0.01
处理间	1	25.74	25.74	45.81^**	3.86	6.69
处理内	619	168.04	0.56
总变异	620	193.79

处理	平均严重度	差异显著性
处理	平均严重度	α=0.05	α=0.01
不携带Fhb1	3.2473	a	A
携带Fhb1	2.3105	b	B