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Chinese Agricultural Science Bulletin ›› 2021, Vol. 37 ›› Issue (4): 98-104.doi: 10.11924/j.issn.1000-6850.casb20200300201

Special Issue: 生物技术 小麦 农业生态

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Research of Resistance in ‘Zhoumai’ Wheat Cultivars to Fusarium Head Blight (FHB) with Fhb1 Gene

Li Nannan(), Li Shuncheng, Han Yulin(), Zou Shaokui, Du Xiaoyu, Yang Guangyu, Wang Lina, Zhang Qian, Lv Yongjun   

  1. Zhoukou Academy of Agricultural Sciences/ Wheat Germplasm Improvement Engineering Research Center of Henan Province/ Key Laboratory of Crop Molecular Breeding and Bioreactor of Henan Province, Zhoukou Henan 466001
  • Received:2020-03-13 Revised:2020-04-27 Online:2021-02-05 Published:2021-01-25
  • Contact: Han Yulin E-mail:18638099528@163.com;Hanyulin2005@126.com

Abstract:

To accelerate the improvement of wheat resistance to fusarium head blight (FHB) in ‘Zhoumai’ wheat, we used the local main wheat varieties (lines) ‘Zhoumai 22’, ‘Zhoumai 32’ and ‘Zhou11550’ as the female parents and the ‘Ningmai 9’, ‘Shengxuan 6’, ‘Yangmai 21’, which are the FHB resistant materials in the middle and lower reaches of the Yangtze River, as male parents to make a series of hybrid combinations. Then we got 621 selected materials in F3-F6 offspring. In the field, we used different FHB strains from Jiangsu and Henan to identify the resistance to FHB through inoculating spores into spikelets by single flower drip method. Meanwhile, the molecular detection of the progenitor materials was carried out by using the closely linked diagnostic marker His-InDel of the main gene Fhb1. Field inoculation results showed that 23.9% of the total offspring from Jiangsu had high or moderate resistance to FHB, while this rate was 35.1% in the offspring of Henan. The resistance of the offspring materials was significantly improved compared with their parents, which were susceptible to FHB. And the resistance of the materials to strains from Jiangsu was lower than that from Henan. Molecular detection results showed that the resistance to FHB had a significant difference in materials containing Fhb1 or not, indicating that Fhb1 gene marker assisted selection could be used to improve the resistance of wheat cultivars to FHB efficiently.

Key words: wheat, fusarium head blight (FHB), Fhb1 gene, molecular identification, field resistance

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