Inhibitory Effect of Slightly Acidic Hypochlorite Water on Spore Germination of Two Pathogenic Fungi of Strawberry and Its Effect on Strawberry Rhizosphere Soil Fungal Diversity

doi:10.11924/j.issn.1000-6850.casb2024-0473

Abstract

Abstract:

In order to explore the effect and application technology of slightly acidic hypochlorite water (SAHW) in strawberry disease control, the fungal inhibition tests (inhibitory effect on the spore germination) was used in this paper to study the antifungal activity of SAHW against the two disease pathogens (Fusarium oxysporum and Botrytis cinerea). Illumina Miseq sequencing technology was also used to analyze the fungal community structure and diversity of the strawberry rhizosphere soil during the planting, early blooming and full-fruiting stages of strawberry before and after SAHW treatment. The results showed that the germination rates of F. oxysporum and B. cinerea spores cultured in the 25 mg/kg SAHW treatment group were both lower than 10%, while the germination rate of the control group cultured by purified water was close to 100%. A total of 15 phyla, 45 classes, 96 orders, 220 families and 405 genera were identified by Illumina Miseq sequencing. Among them, Ascomycota, Mortierellomycota and Basidiomycota were the dominant fungi. At the genus classification level, the relative abundance of Aspergillus (2.21%-53.54%) and Trichocladium (4.76%-26.54%) was relatively high. The relative abundance of the phytopathogenic fungus Aspergillus reached the highest value during the flowering stage and then showed a decreasing trend in the treatment groups. In addition, Penicillium, Fusarium and Colletotrichum gradually accumulated in the strawberry rhizosphere soil treated with purified water and reached the highest level during the fruiting period, but remained at a low level in the strawberry rhizosphere soil treated with SAHW. This study preliminarily revealed the antifungal effect of SAHW and its impact on the diversity of strawberry rhizosphere fungi, providing a scientific basis for comprehensive prevention and control of strawberry diseases.

Key words: strawberry, Botrytis cinerea, Fusarium oxysporum, slightly acidic hypochlorite water, rhizosphere soil, fungal diversity, Illumina Miseq sequencing

LIU Zheng, ZHANG Jingjing, ZHAO Namula, XU Pengbo, LIAN Hongli, XU Xiaofeng, ZHANG Liqing. Inhibitory Effect of Slightly Acidic Hypochlorite Water on Spore Germination of Two Pathogenic Fungi of Strawberry and Its Effect on Strawberry Rhizosphere Soil Fungal Diversity[J]. Chinese Agricultural Science Bulletin, 2025, 41(12): 113-122.

Figures/Tables 9

References 28

[1]	高清山. 我国草莓产业的现状分析及发展趋势研究[J]. 果树资源学报, 2024, 5(5):79-82.
[2]	MENG L, AUDENAERT K, VAN LABEKE M C, et al. Detection of Botrytis cinerea on strawberry leaves upon mycelial infection through imaging technique[J]. Scientia horticulturae, 2024, 330(1):113071-113074.
[3]	HENRY P M, HAUGLAND M, LOPEZ L, et al. The potential for Fusarium oxysporum f. sp. fragariae, cause of fusarium wilt of strawberry, to colonize organic matter in soil and persist through anaerobic soil disinfestation[J]. Plant pathology, 2020, 69(7):1218-1226.
[4]	KAREEM F, ELSHAHAWY I E, ELGAWAD M M. Application of Bacillus pumilus isolates for management of black rot disease in strawberry[J]. Egyptian journal of biological pest control, 2021, 31(1):1-5.
[5]	顾峥嵘, 陈晓, 翁蔚宗, 等. 次氯酸临床研究及使用进展[J]. 世界复合医学, 2015, 1(4):336-339.
[6]	堀田国元, 郭永明. 酸性电解水的基础、应用及发展动向[J]. 中国护理管理, 2008, 8(4):7-11.
[7]	张雪丽, 苏静, 胡瑞雪, 等. 微酸性次氯酸水对口腔印模的消毒效果观察[J]. 中国消毒学杂志, 2023, 40(11):810-813. doi: 10.11726/j.issn.1001-7658.2023.11.004
[8]	魏肖鹏, 董宇, 孙娟娟, 等. 电解水对黄瓜生长,果实品质及黄瓜霜霉病和白粉病防效的影响[J]. 植物保护学报, 2016, 43(5):9-16.
[9]	肖伟, 张嘉雯, 黄霜, 等. 酸性电解水对早期番茄晚疫病防治具有良好效果[J]. 长江蔬菜, 2021(22):26-27.
[10]	刘琪, 张鑫, 任彬元, 等. 电解水在我国植物保护上的应用进展[J]. 中国植保导刊, 2020, 40(1):35-39,66.
[11]	刘李岚, 肖伟, 陈珂, 等. 利用电解水农业技术体系有效防治早春番茄叶霉病[J]. 长江蔬菜, 2021(12):24-25.
[12]	田春英, 张建文, 王文合, 等. 酸性电解水对番茄灰霉病的防效及对番茄品质的影响[J]. 中国植保导刊, 2021, 41(10):61-64.
[13]	肖伟, 张彩君. 在电解水农业技术体系中不同有机硅肥施用方式对番茄吸收硅和糖度的影响[J]. 长江蔬菜, 2020(12):1231-1232.
[14]	肖伟, PERKASH O M, 陈珂, 等. 酸性电解水促进番茄叶片硅吸收与提高植株抗性的作用[J]. 长江蔬菜, 2019, 1(20):22-23.
[15]	周然, 谢晶, 高启耀, 等. 微酸性电解水结合壳聚糖对水蜜桃护色保鲜的效果[J]. 农业工程学报, 2012, 28(18):281-286.
[16]	KOIDE S, SHITANDA D, NOTE M, et al. Effects of mildly heated, slightly acidic electrolyzed water on the disinfection and physicochemical properties of sliced carrot[J]. Food control, 2011, 22(3/4):452-456.
[17]	郝建雄, 李里特, 肖卫华. 电生功能水对番茄的保鲜[J]. 食品科技, 2006, 1(2):100-102.
[18]	陈倩茹, 于一凡, 照那木拉, 等. 微酸性次氯酸水对鲜切莴笋的保鲜作用效果[J]. 现代食品科技, 2024, 40(5):134-141.
[19]	申顺善, 张莹莹, 张维娜, 等. 绿针假单胞菌HL5-4对番茄灰霉菌的抑制活性及其定殖能力[J]. 园艺学报, 2016, 43(6):1195-1202.
[20]	强然, 张岱, 杨喆, 等. 解淀粉芽胞杆菌L19对马铃薯枯萎病菌的抑制及植株的促生作用[J]. 园艺学报, 2024, 1(4):875-892.
[21]	CHEN S F, ZHOU Y Q, CHEN Y R, et al. Fastp: an ultra-fast all-in-one FASTQ preprocessor[J]. Bioinformatics, 2018, 34(17):i884-i890.
[22]	MAGOČ T, SALZBERG S L. FLASH: fast length adjustment of short reads to improve genome assemblies[J]. Bioinformatics, 2011, 27(21):2957-2963. doi: 10.1093/bioinformatics/btr507 pmid: 21903629
[23]	余朝阁, 隋心意, 丁勇, 等. 微酸性次氯酸对番茄灰霉病和灰叶斑病的抑制作用[J]. 沈阳农业大学学报, 2022, 53(6):658-664.
[24]	AMAIKE S, KELLER N P. Aspergillus flavus[J]. Annual review of phytopathology, 2011, 49(1):107-133.
[25]	HUSSEIN M A, SAID A H, YASSEIN A S. Mycobiota associated with strawberry fruits, their mycotoxin potential and pectinase activity[J]. Mycology, 2020, 11(2):158-166. doi: 10.1080/21501203.2020.1759719 pmid: 32923023
[26]	ZHANG Y, YU H, HU M, et al. Fungal pathogens associated with strawberry crown rot disease in China[J]. Journal of fungi, 2022, 8(11):1161-1163.
[27]	TIAN Y, ZHAO Y, FU X, et al. Isolation and identification of Talaromyces sp. strain Q2 and its biocontrol mechanisms involved in the control of Fusarium wilt[J]. Frontiers in microbiology, 2021, 12(1):724842-724843.
[28]	EVUEH G, OSEMWEGIE O. Evaluation phylloplane fungi as biocontrol agent of Corynespora leaf disease of rubber (Hevea brasiliensis Muell. Arg.)[J]. World journal of fungal and plant biology, 2011, 2(1):1-5.

样品名称	有效读长	序列平均长度	碱基数量	Q₂₀	Q₃₀
CK₁	62567	138	15601704	96.33	99.24
CK₂	76389	138	19710152	96.42	99.42
CK₃	77059	138	19062375	97.48	99.58
T₁	66905	139	17007094	96.89	99.48
T₂	56173	138	14212229	96.87	99.38
T₃	75374	138	18831461	97.56	99.59

样品名称	有效读长	序列平均长度	碱基数量	Q₂₀	Q₃₀
CK₁	62567	138	15601704	96.33	99.24
CK₂	76389	138	19710152	96.42	99.42
CK₃	77059	138	19062375	97.48	99.58
T₁	66905	139	17007094	96.89	99.48
T₂	56173	138	14212229	96.87	99.38
T₃	75374	138	18831461	97.56	99.59