Effects of One-time Application of Nitrogen Fertilizer on Growth and Photosynthetic Characteristics of Paeonia lactiflora

doi:10.11924/j.issn.1000-6850.casb2024-0561

Abstract

Abstract:

To clarify the effects of one-time application of nitrogen (N) fertilizer on the growth of Paeonia lactiflora, and provide a theoretical basis for its nutrient management, three N levels (0, 1.8, 3 g/plant) were set up to study the response of growth and photosynthetic characteristics of P. lactiflora ‘Heihaibotao’ to the one-time application of N fertilizer in stem-leaf growth period by a pot culture experiment. The results showed that compared with the 0 N application treatment, 1.8 g/plant N application could significantly increase the flower diameter, root length, and root volume, promote the accumulation and distribution ratio of dry matter in the above-ground part, and increase the root-shoot ratio, while the effects of N application on plant height, stem diameter and dry matter accumulation in the above-ground part did not reach a significant level. The growth of above- and under-ground parts was inhibited by one-time N application of 3 g/plant. N application significantly increased the net photosynthetic rate, transpiration rate and stomatal conductance of plants, and decreased the intercellular CO₂ concentration. N application had no significant effect on N concentration in above- and under-ground parts and 1.8 g/plant N application significantly increased N uptake in above- and under-ground parts and N distribution ratio in under-ground parts. In conclusion, the one-time application of appropriate N fertilizer during the stem-leaf growth period of P. lactiflora is beneficial to increasing flower diameter, improving the ornamental value, and promoting root growth and development. However, excessive N application inhibits the growth of plants.

Key words: Paeonia lactiflora, nitrogen fertilizer, root, growth characteristic, photosynthetic characteristic, dry matter, nutrient absorption, nutrient management, root-shoot ratio

WANG Xin, ZHANG Xinxin, MI Ming, LI Shuo, LI Haojian, MA Hailin, SI Dongxia. Effects of One-time Application of Nitrogen Fertilizer on Growth and Photosynthetic Characteristics of Paeonia lactiflora[J]. Chinese Agricultural Science Bulletin, 2025, 41(7): 61-66.

Figures/Tables 7

References 31

[1]	王慧娟, 王二强, 符真珠, 等. 芍药不同品种群种质资源表型多样性分析[J]. 河南农业科学, 2022, 51(12):110-121.
[2]	朱炜, 曹津津, 陈曦, 等. 芍药新品种‘粉罗裙’‘千重粉黛’‘笑春风’和‘碧叶粉荷’[J]. 园艺学报, 2023, 50(2):457-458. doi: 10.16420/j.issn.0513-353x.2021-0006
[3]	于晓南, 宋焕芝, 郑黎文. 国外观赏芍药育种与应用及其启示[J]. 湖南农业大学学报(自然科学版), 2010, 36(S2):159-162.
[4]	LI W X, SONG X M, HUA Y M, et al. Effects of different harvest times on nutritional component of herbaceous peony flower petals[J]. Journal of chemistry, 2020(3):1-7.
[5]	孙静, 姜宇, 陶俊. 牡丹与芍药的食用研究进展[J]. 江苏农业科学, 2018, 46(11):10-14.
[6]	YANG L P, ZHOU M D, ZU M T, et al. Comprehensive microbiome and metabolome analyses reveal the medicinal components of Paeonia lactiflora[J]. Plants, 2023, 12(8):1612.
[7]	TANG P, NI Y, LI J, et al. The complete mitochondrial genome of Paeonia lactiflora Pall. (Saxifragales: Paeoniaceae): Evidence of gene transfer from chloroplast to mitochondrial genome[J]. Genes, 2024, 15(2):239.
[8]	ZHU M T, PENG Y, GAO J Z, et al. Exploring the relationship between pollen viability and inclusion in Paeonia lactiflora after cryopreservation[J]. Cryobiology, 2024,115:104867.
[9]	BERGMANN B A, JAHNKE N J, DOLE J M, et al. Impacts of carbohydrate pulses and short-term sub-zero temperatures on vase life and quality of cut Paeonia lactiflora Pall. hybrids[J]. Postharvest biology and technology, 2020,161.
[10]	张捷, 谢军, 孙辑凯, 等. 芍药播种繁殖中种子破眠技术的研究进展[J]. 分子植物育种, 2018, 16(13):4380-4386.
[11]	苏本营, 周美华, 许宏, 等. 观赏芍药在北京山区的生理生态特征表现[J]. 北方园艺, 2023(19):65-70.
[12]	赵俊福, 何晗颖, 张明泽, 等. 基于“3414”试验的切花芍药氮磷钾推荐施肥量研究[J]. 中国土壤与肥料, 2024(5):70-77.
[13]	XU G H, TAKAHASHI H. Improving nitrogen use efficiency: From cells to plant systems[J]. Journal of experimental botany, 2020, 71(15):4359-4364. doi: 10.1093/jxb/eraa309 pmid: 32710784
[14]	BANGER K, WAGNER-RIDDLE C, GRANT B B, et al. Modifying fertilizer rate and application method reduces environmental nitrogen losses and increases corn yield in Ontario[J]. Science of the total environment, 2020,722:137851.
[15]	YU B, WU K N. Effects of nitrogen application on plant growth and soil inorganic nitrogen content in alpine grasslands[J]. Agricultural biotechnology, 2018, 7(6):130-135.
[16]	ZHANG C, HUANG X, ZHANG X, et al. Effects of biochar application on soil nitrogen and phosphorous leaching loss and oil peony growth[J]. Agricultural water management, 2021,255:107022.
[17]	DING Z J, LI J T, HUANG F, et al. One-time root-zone fertilization of controlled-release urea increases nitrogen use efficiency while reducing nitrogen loss risk in a ratoon rice field under two irrigation practices[J]. Nutrient cycling in agroecosystems, 2023, 128(1):13-33.
[18]	牛立军. 芍药切花露地及设施生产栽培技术研究[D]. 北京: 北京林业大学, 2010.
[19]	李兆鹏, 姜楠南, 孙音, 等. 氮水平对盆栽芍药生长发育及氮代谢的影响[J]. 山东林业科技, 2019, 49(5):13-17.
[20]	苗庆选, 怀婷婷, 刘春晓, 等. 氮素不同用量对核桃幼苗生长及光合特性的影响[J]. 山东农业科学, 2023, 55(1):106-111.
[21]	DUAN J Z, SHAO Y H, HE L, et al. Optimizing nitrogen management to achieve high yield, high nitrogen efficiency and low nitrogen emission in winter wheat[J]. Science of the total environment, 2019,697:134088.
[22]	韩建, 张玉铭, 何红波, 等. 长期不同施氮量下微生物残体氮对土壤氮库稳定性和玉米氮素吸收的影响[J]. 中国生态农业学报(中英文), 2024, 32(5):766-779.
[23]	何晗颖, 赵俊福, 刘淑杰, 等. 盆栽牡丹生长和生理特性对现蕾期氮肥的响应[J]. 山东农业科学, 2024, 56(3):99-105.
[24]	SUN T, ZHOU J, FU Y, et al. Soil nitrogen availability mediates the positive effects of intercropping on soil organic carbon at global scales[J]. Soil & tillage research, 2024,239:106063.
[25]	孙晓梅, 赵盼盼, 赵琳, 等. 容器栽培对芍药生长发育、干物质积累及分配的影响[J]. 浙江农业学报, 2014, 26(3):632-637.
[26]	霍常富, 孙海龙, 范志强, 等. 根系氮吸收过程及其主要调节因子[J]. 应用生态学报, 2007(6):1356-1364.
[27]	齐晓丽, 刘磊, 崔克辉. 水稻氮素吸收利用与根系特征相互关系的研究进展[J]. 植物生理学报, 2024, 60(3):441-450.
[28]	RASMUSSEN S I, DRESBØLL B D, THORUP-KRISTENSEN K. Winter wheat cultivars and nitrogen (N) fertilization- Effects on root growth, N uptake efficiency and N use efficiency[J]. European journal of agronomy, 2015,68:38-49.
[29]	LI J J, LIU X Y, YAO Q, et al. Tolerance and adaptation characteristics of sugar beet (Beta vulgaris L.) to low nitrogen supply[J]. Plant signaling & behavior, 2023, 18(1):2159155.
[30]	郭案铭, 吴昆仑, 姚晓华, 等. 施氮水平对青稞氮素吸收利用率、根系形态和产量的影响[J]. 麦类作物学报, 2024, 44(6):787-794.
[31]	满欣雨, 郭亚蓉, 葛红娟, 等. 植物地下部与地上部远端相互调控研究进展[J]. 植物生理学报, 2018, 54(6):931-942.

施肥后天数/d	株高/cm			茎粗/mm
施肥后天数/d	N₀	N_1.8	N_3.0	N₀	N_1.8	N_3.0
2	56.00±1.00a	52.00±1.00b	51.00±1.00b	9.96±0.58a	9.38±0.10a	9.22±0.10a
7	63.67±1.15a	61.33±1.53a	58.33±1.53b	10.49±0.59a	10.11±0.07a	9.35±0.09b
11	67.67±1.53a	65.33±1.53a	61.33±1.53b	10.66±0.63a	10.36±0.09a	10.29±0.07a
15	71.33±1.53a	70.00±1.00a	62.67±1.53b	10.73±0.62a	10.65±0.09a	10.39±0.07a
18	71.67±1.61a	71.00±1.00a	63.33±1.61b	11.32±0.59a	10.86±0.09ab	10.47±0.07b

施肥后天数/d	株高/cm			茎粗/mm
施肥后天数/d	N₀	N_1.8	N_3.0	N₀	N_1.8	N_3.0
2	56.00±1.00a	52.00±1.00b	51.00±1.00b	9.96±0.58a	9.38±0.10a	9.22±0.10a
7	63.67±1.15a	61.33±1.53a	58.33±1.53b	10.49±0.59a	10.11±0.07a	9.35±0.09b
11	67.67±1.53a	65.33±1.53a	61.33±1.53b	10.66±0.63a	10.36±0.09a	10.29±0.07a
15	71.33±1.53a	70.00±1.00a	62.67±1.53b	10.73±0.62a	10.65±0.09a	10.39±0.07a
18	71.67±1.61a	71.00±1.00a	63.33±1.61b	11.32±0.59a	10.86±0.09ab	10.47±0.07b

施肥后天数/d	株高差/cm			茎粗差/mm
施肥后天数/d	N₀	N_1.8	N_3.0	N₀	N_1.8	N_3.0
0~2	7.67±0.58a	5.00±1.00b	5.00±0.00b	0.16±0.06a	0.17±0.02a	0.13±0.04a
2~7	7.67±0.58a	9.33±1.15a	7.33±1.15a	0.53±0.01b	0.73±0.10a	0.53±0.06b
7~11	4.00±1.00a	4.00±0.00a	3.00±0.00a	0.17±0.05c	0.25±0.03b	0.94±0.02a
11~15	3.67±0.58a	4.67±0.58a	1.33±0.58b	0.06±0.02b	0.28±0.04a	0.10±0.03b
15~18	0.33±0.29b	1.00±0.00a	0.67±0.23ab	0.60±0.04a	0.21±0.01b	0.09±0.02c
总增量	23.33±1.15a	24.00±1.00a	17.33±2.08b	1.53±0.07b	1.65±0.17ab	1.78±0.09a

施肥后天数/d	株高差/cm			茎粗差/mm
施肥后天数/d	N₀	N_1.8	N_3.0	N₀	N_1.8	N_3.0
0~2	7.67±0.58a	5.00±1.00b	5.00±0.00b	0.16±0.06a	0.17±0.02a	0.13±0.04a
2~7	7.67±0.58a	9.33±1.15a	7.33±1.15a	0.53±0.01b	0.73±0.10a	0.53±0.06b
7~11	4.00±1.00a	4.00±0.00a	3.00±0.00a	0.17±0.05c	0.25±0.03b	0.94±0.02a
11~15	3.67±0.58a	4.67±0.58a	1.33±0.58b	0.06±0.02b	0.28±0.04a	0.10±0.03b
15~18	0.33±0.29b	1.00±0.00a	0.67±0.23ab	0.60±0.04a	0.21±0.01b	0.09±0.02c
总增量	23.33±1.15a	24.00±1.00a	17.33±2.08b	1.53±0.07b	1.65±0.17ab	1.78±0.09a

处理	根长/m	根表面积/m²	根平均直径/mm	根体积/cm³
N₀	27.00±0.04b	0.12±0.01ab	1.33±0.05ab	43.25±3.18b
N_1.8	27.57±0.12a	0.14±0.02a	1.37±0.11a	68.69±6.08a
N_3.0	26.37±0.05c	0.10±0.03b	1.21±0.05b	44.63±2.64b